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Bacillus subtilis and application thereof in degradation of zearalenone

A technology of Bacillus subtilis and zearalenone, applied in the field of microorganisms, can solve the problems of immunotoxicity, liver toxicity, genotoxicity, etc., and achieve the effects of reducing harm, strong stress resistance, and high detoxification activity

Pending Publication Date: 2022-01-11
FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to reproductive toxicity, zearalenone may also cause different problems such as hepatotoxicity, immunotoxicity, and genotoxicity

Method used

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  • Bacillus subtilis and application thereof in degradation of zearalenone
  • Bacillus subtilis and application thereof in degradation of zearalenone
  • Bacillus subtilis and application thereof in degradation of zearalenone

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Screening, isolation and identification of embodiment 1 bacterial strain

[0045] The main process of screening, isolation and identification of Bacillus subtilis ZJ-2019-1 is as follows:

[0046] 1. Take 10g of feces samples from a farm in Xingtai, Hebei, and place them in 100mL of improved LB liquid medium, 160r / min, 37°C for 24h enrichment culture to obtain bacterial liquid. Place the bacterial solution at 80°C for 10 minutes, spread it on the plate, and incubate at 37°C for 24 hours.

[0047] 2. Purification of strains

[0048] A single colony was picked for staining and microscopic examination, and the Gram-positive and rod-shaped bacteria were streaked, purified, and preserved.

[0049] 3. Screening of bacterial strains

[0050]Cultivate the strain according to the culture conditions in the above 1 to obtain a fermented liquid, take the fermented liquid and mix it with a certain concentration of zearalenone, and leave it for a certain period of time to measure ...

Embodiment 2

[0055] The cultivation of embodiment 2 Bacillus subtilis ZJ-2019-1 and preparation of fermented liquid

[0056] The cultivation method of Bacillus subtilis ZJ-2019-1 is as follows:

[0057] (1) After activation, Bacillus subtilis ZJ-2019-1 was inoculated in the seed medium for cultivation to obtain seed liquid, and the concentration of live bacteria of Bacillus subtilis ZJ-2019-1 in the seed liquid was 10 9 cfu / mL;

[0058] The formula of the seed medium is as follows: glucose 1%, yeast extract 1%, peptone 2%, manganese sulfate 0.005%, dipotassium hydrogen phosphate 0.2%, magnesium sulfate 0.1%, pH 7.0.

[0059] (2) inoculating the seed liquid obtained in step (1) in the fermentation medium according to the inoculation ratio of 2% to carry out fermentation culture to obtain a fermentation liquid;

[0060] The fermentation medium is an improved LB liquid medium;

[0061] The fermentation culture conditions are: fermentation temperature 37°C, rotation speed 180r / min, fermenta...

Embodiment 3

[0062] The preparation of the fermentation product of embodiment 3 Bacillus subtilis ZJ-2019-1

[0063] The preparation method of the fermentation product of Bacillus subtilis ZJ-2019-1 is as follows:

[0064] (1) After activation, Bacillus subtilis ZJ-2019-1 was inoculated in the seed medium for cultivation to obtain seed liquid, and the concentration of live bacteria of Bacillus subtilis ZJ-2019-1 in the seed liquid was 10 9 cfu / mL;

[0065] The formula of the seed medium is as follows: glucose 1%, yeast extract 1%, peptone 2%, manganese sulfate 0.005%, dipotassium hydrogen phosphate 0.2%, magnesium sulfate 0.1%, pH 7.0.

[0066] (2) inoculating the seed liquid obtained in step (1) in the fermentation medium according to the inoculation ratio of 2% to carry out fermentation culture to obtain a fermentation liquid;

[0067] The fermentation medium is modified LB liquid medium.

[0068] The fermentation culture conditions are: fermentation temperature 37°C, rotation speed 1...

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Abstract

The invention relates to the technical field of microorganisms, in particular to bacillus subtilis and application thereof in degradation of zearalenone. The bacillus subtilis ZJ-2019-1 provided by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacillus subtilis ZJ-2019-1 is CGMCC No.23636. The strain can efficiently degrade zearalenone toxin, the degradation rate can reach about 100%, the detoxification activity is high, the strain can be used for degrading zearalenone toxin in feed and other products, harm of zearalenone to animal reproductive performance and the like is greatly reduced, animal production performance is improved, and sensory characteristics of feed cannot be affected; and meanwhile, the bacillus subtilis has no pollution to the environment and has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a bacillus subtilis and its application in degrading zearalenone. Background technique [0002] Mycotoxin contamination is a common and serious problem in the current feed field. Among them, toxins such as aflatoxin, deoxynivalenol and zearalenone seriously threaten the health of animals. At present, the degradation methods of mycotoxins mainly include physical detoxification, chemical detoxification and microbial detoxification. Among them, the application of physical and chemical detoxification is more common, but these two methods may affect the sensory of feed characteristics and qualities, etc., thereby limiting its widespread use. Microbial detoxification method is an emerging method that has only been studied in recent years. The advantage of this method is that it can remove mycotoxins without causing significant impact on feed quality and sensory characteristics,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L5/20A23K10/18A23K50/30C12R1/125
CPCC12N1/20A23L5/28A23K10/18A23K50/30A23V2400/169
Inventor 王金全韩冰张俊楠王振龙陶慧郑云朵赵鹏刘杰
Owner FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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