Method for detecting plant root endophyte containing autotrophic arsenic oxidation function microorganisms

Abstract

The invention discloses a method for detecting plant root endophyte containing autotrophic arsenic oxidation function microorganisms. Through a comprehensive means of combining separation, culture and DNA-SIP technologies, the functional microorganisms participating in the heavy (similar) metal oxidation-reduction process in the plant endophyte can be accurately distinguished. On the basis of efficiently extracting and separating plant tissue endophyte, endophyte liquid obtained through endophyte liquid separation is subjected to enlarged culture by using a liquid culture medium, so that the microorganism abundance and activity of the endophyte liquid are ensured. Meanwhile, DNA-SIP is used as a means, microorganisms for driving heavy metal redox can be directly and effectively anchored, q-PCR amplification and 16S rRNA high-throughput sequencing are further utilized, and the heavy metal redox function microorganism class group abundance change in a microcosm system and metabolism information of key function endophyte microorganisms are analyzed, and whether the plant endophyte community has the functional bacteria for driving the heavy (similar) metal redox process or not can be accurately judged.

Description

technical field [0001] The invention belongs to the field of microbial ecology, and in particular relates to a method for detecting microorganisms containing autotrophic arsenic oxidation function in plant root endophytes. Background technique [0002] Plant endophytes mainly live in plants and can exert various biological effects on host plants. Studies have shown that plant endophytes can enhance the resistance of host plants to abiotic stresses such as temperature, water, and metal pollution, as well as biotic stresses such as insects, pathogenic bacteria, and nematodes, and at the same time provide plants with the nutrients they need, such as nitrogen sources. Promote plant growth and regulate plant physiological processes. [0003] In recent years, plant endophytes have received widespread attention at home and abroad due to their unique ecology. A large number of studies have shown that endophytes participate in the physiological functions and various metabolisms of p...

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Problems solved by technology

[0004] Since the vast majority of plant endophytes cannot be obtained by pure culture techniques, although the community composition of plant endophytes has been discovered through molecular biology techniques, the heavy metal tolerance mechanism of plant root endophytes is still unclear, and further research is needed. Ecological interaction effects between functional endophytes and heavy metal polluted soil-plants

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