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Cervical cancer carcinogenesis assessment model and construction method

A technology for cervical cancer and cancerization, applied in the field of cervical cancer cancerization level evaluation model and construction, can solve the problems of inaccurate evaluation of cervical cancer, achieve good capture stability and uniformity, reduce sequencing cost, sensitivity and specificity high effect

Active Publication Date: 2022-04-19
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies of the above-mentioned prior art, the problem to be solved by the present invention is: how to provide a cervical cancer canceration level assessment model and a construction method to solve the problem of inaccurate assessment of cervical cancer

Method used

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  • Cervical cancer carcinogenesis assessment model and construction method
  • Cervical cancer carcinogenesis assessment model and construction method
  • Cervical cancer carcinogenesis assessment model and construction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 Establishment of DNA methylation detection method

[0061] 1. Methylation probe design

[0062] (1) Methylation mark gene screening

[0063] Screen the methylated regions with obvious methylation characteristics related to cervical cancer, take the reference sequence of each region (reference genome version hg19), remove the sequence of the repeated region, and use the RepeatMask software to analyze the repeated sequence to obtain Table 1. SEQID No.: 1 -45 gene sequences.

[0064] Table 1 Gene list of SEQ ID No.: 1-45

[0065] ADCYAP1 CDKN2A Jam3 PCDHA4 TERT AJAP1 DAPK1 KCNIP4 POU4F3 TIMP3 ANKRD18CP DLX1 LHX8 RARB WIF1 APCs EPB41L3 LMX1A RNASEH2A ZNF671 ASTN1 FAM19A4 MAL RUBCNL ZSCAN1 CADM1 FANCI MIR124-1 RXFP3 CCNA2 FHIT MIR124-2 SLIT2 CDH1 GATA4 MIR124-3 SOX1 CDH13 GFRA1 PAX1 SOX17 CDH6 HS3ST2 PCDHA13 ST6GALNAC5

[006...

Embodiment 2

[0114] Using the method of Example 1 to establish a methylation detection classification model / prediction model

[0115] Collect 80 samples of people with pathological results of CIN2 and above and people with CIN1 and below (all with the informed consent of the patients) and detect according to the method of Example 1, and establish a methylation classification model.

[0116] The probe and method of Example 1 targeted enrichment of the target region library through high-throughput sequencing, and the results showed that: the probe, reagent and method of Example 1 had a high capture rate for the target region, reaching more than 50%; The effective average sequencing depth is above 1000X; the 20X coverage reaches above 99% (see Table 4 for some data).

[0117] Table 4 Utilizes the method of the present invention to detect the quality control data result of sample

[0118]

[0119] By extracting CpG sites from high-throughput sequencing data for 80 samples, screening CpG hi...

Embodiment 3

[0124] The classification model / prediction model of 40 examples of sample verification embodiment 2

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Abstract

The invention relates to the field of molecular biology detection, in particular to a cervical cancer carcinogenesis evaluation model and a construction method; by screening existing cervical cancer genes and their methylation regions, designing probes, and extracting cervical cancer tumor tissue Cellular DNA; after fragmentation of cervical cell DNA, carry out end repair and connection of joints, construct cervical cell DNA methylation library, and carry out targeted enrichment of the library; perform bioinformatics analysis on the processed cervical cell DNA methylation library, Using the logistic regression algorithm to build an evaluation model, using clinical samples to verify the evaluation model; through this evaluation model for the evaluation of cervical cancer carcinogenesis, the coincidence rate can reach 92.5%, the clinical sensitivity is 0.888889, the clinical specificity is 0.954545, and the AUC is 0.9271, which can effectively reduce Overtreatment to reduce cervical cancer morbidity and mortality.

Description

technical field [0001] The invention relates to the field of molecular biology detection, in particular to a cervical cancer carcinogenesis level evaluation model and a construction method. Background technique [0002] Cervical cancer is the fourth most common cancer in women worldwide and the leading cause of cancer death among women in developing countries. There are about 130,000 new cases of cervical cancer in my country every year, accounting for 28% of the total number of new cases of cervical cancer in the world. On July 6, 2021, the World Health Organization (WHO) released guidelines for the screening and treatment of cervical precancerous lesions in cervical cancer prevention, recommending optimization of diagnostic tools and screening options to promote cervical cancer prevention and save more lives. [0003] Current cervical cancer screening includes cervical cytology testing and human papillomavirus (HPV) testing. Cytology tests include Pap smears and TCT liqu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12Q1/6858G16B5/00G16B30/10G16B40/00G16B50/00
CPCC12Q1/6886C12Q1/6858G16B5/00G16B30/10G16B40/00G16B50/00C12Q2600/154C12Q2535/122C12Q2523/125C12Q2537/165
Inventor 伍建姬晓雯王海丽刘娜韩路
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI