Check patentability & draft patents in minutes with Patsnap Eureka AI!

Primer probe combination for rapid nucleic acid detection of novel coronavirus 2019-nCoV through isothermal amplification and application thereof

A 2019-ncov, isothermal amplification technology, applied in the field of nucleic acid detection, can solve the problems of low sensitivity, complex primer design and reaction system, prone to false positives, etc., achieve high application value, good parallelism and repeatability, improve The effect of sensitivity and specificity

Pending Publication Date: 2022-01-18
SHANGHAI BIOTECAN PHARMA +2
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some researchers use constant temperature amplification schemes for related detection, but there are problems such as low sensitivity, prone to false positives, and complex primer design and reaction system

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primer probe combination for rapid nucleic acid detection of novel coronavirus 2019-nCoV through isothermal amplification and application thereof
  • Primer probe combination for rapid nucleic acid detection of novel coronavirus 2019-nCoV through isothermal amplification and application thereof
  • Primer probe combination for rapid nucleic acid detection of novel coronavirus 2019-nCoV through isothermal amplification and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] This embodiment provides a combination of primers and probes for rapid nucleic acid detection of novel coronavirus 2019-nCoV by constant temperature amplification, which includes specific amplification and detection Specific primer pairs and probes for ORF1ab gene and nucleocapsid protein N gene of 2019-nCoV;

[0083] The structure of the probe includes a 5' end sequence connected sequentially from the 5' end to the 3' end, a fluorescent generating group, tetrahydrofuran, a spacer sequence, a fluorescence quenching group, a 3' end sequence and a C3 blocking group .

[0084] The specific primer pair for amplifying the ORF1ab gene includes the nucleotide sequence shown in SEQ ID No.1-2, and the probe for detecting the ORF1ab gene includes the nucleotide sequence shown in SEQ ID No.3;

[0085] The specific primer pair for amplifying the nucleocapsid protein N gene includes the nucleotide sequence shown in SEQ ID No.4~5, and the probe for detecting the nucleocapsid protein...

Embodiment 2

[0102] This embodiment provides a kit for isothermal amplification and rapid nucleic acid detection of novel coronavirus 2019-nCoV. Primer-probe combination, buffer, enzyme mix, activator, positive control and negative control for detection of novel coronavirus 2019-nCoV.

[0103] The primer probe combination of described isothermal amplification rapid nucleic acid detection novel coronavirus 2019-nCoV is made into primer probe mixture;

[0104] The enzyme mixture includes a recombinase that binds single-stranded nucleic acids, a single-stranded DNA binding protein, a strand-displacing DNA polymerase, and a helicase;

[0105] The primer-probe mixture, enzyme mixture and buffer are prepared in a volume ratio of 3:5:11 into a premix;

[0106] The activator is Mg 2+ ;

[0107] The negative control is sterile deionized water;

[0108] The positive control is a self-prepared positive standard product, wherein the enterprise positive reference product of ORF1ab gene, N gene and ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a primer probe combination for rapid nucleic acid detection of the novel coronavirus 2019-nCoV through isothermal amplification and application thereof. The primer probe combination for rapid nucleic acid detection of the novel coronavirus 2019-nCoV through isothermal amplification comprises a specific primer pair and a probe for specific amplification and detection of an ORF1ab gene and a nuclear shell protein N gene of the 2019-nCoV; the structure of the probe comprises a 5' end sequence, a fluorescence generation group, tetrahydrofuran, an interval sequence, a fluorescence quenching group, a 3 ' end sequence and a C3 blocking group which are sequentially connected from the 5' end to the 3' end. The invention further provides a kit for rapid nucleic acid detection of the novel coronavirus 2019-nCoV through isothermal amplification and a use method of the kit for non-disease diagnosis and / or treatment. The kit has the advantages of excellent sensitivity, specificity and accuracy, high detection efficiency and extremely high application value.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid detection, in particular to a combination of primers and probes for isothermal amplification and rapid nucleic acid detection of novel coronavirus 2019-nCoV and its application. Background technique [0002] The new coronavirus 2019-nCoV belongs to the family Coronaviridae and the genus betacoronavirus. It has an envelope, and the virus particles are round or oval, often pleomorphic, with a diameter of 60-140nm. 7 coronaviruses, and the remaining 6 coronaviruses are HCoV-229E, HCoV-OC43, HCoV-NL63, HCoV-HKU1, SARS-CoV and MERS-CoV. Among them, the first four are more common in the population, less pathogenic, and generally only cause mild respiratory symptoms similar to the common cold; the other two, SARS-CoV and MERS-CoV (Middle East Respiratory Syndrome Coronavirus), can cause severe Respiratory disorders. [0003] Novel coronavirus (2019 novel Coronavirus, 2019-nCoV) is a newly discover...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/70C12Q1/6844C12N15/11
CPCC12Q1/70C12Q1/6844C12Q2563/107C12Q2531/119C12Q2521/107C12Q2545/113Y02A50/30
Inventor 习杨刘晏霖楼敬伟
Owner SHANGHAI BIOTECAN PHARMA
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com