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Solvent-free nucleic acid flexible fluorescent material and preparation method thereof

A fluorescent material, solvent-free technology, applied in the fields of luminescent materials, sugar derivative preparation, chemical instruments and methods, etc., can solve the problems of lack of fluorescent deoxyribonucleic acid flexible materials, restricting the application of deoxyribonucleic acid materials, etc., and achieve easy access to raw materials. Easy to obtain and prepare

Inactive Publication Date: 2022-01-21
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the solvent-free state, there is still a lack of flexible DNA materials with fluorescent properties, which limits the application of DNA materials in some fields, for example, in the field of water-sensitive optoelectronic devices

Method used

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  • Solvent-free nucleic acid flexible fluorescent material and preparation method thereof
  • Solvent-free nucleic acid flexible fluorescent material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Mix 69 μL of 28.7 μM single-stranded deoxyribonucleic acid (22 base units) aqueous solution and 20 μL of 1.5 mM bromide N,N,N-trimethyl-8-(4-(2-(4- octyloxyphenyl)-1,2-diphenylvinyl)phenoxy)octane-1-ammonium aqueous solution was mixed in a centrifuge tube, shaken for 15 minutes, then the mixture was put into a centrifuge, and the Centrifuge for 5 minutes under the centrifugal force of 6000g, discard the supernatant, and freeze-dry the obtained precipitate for 8 hours to obtain the solvent-free nucleic acid flexible fluorescent material of the present invention.

[0026] The prepared solvent-free nucleic acid flexible fluorescent material has a fluorescence emission spectrum stimulated by ultraviolet light (365nm), such as figure 1 shown, according to figure 1 , draw: the solvent-free nucleic acid flexible fluorescent material of the present invention has the maximum fluorescence emission peak at 486nm under the stimulation of ultraviolet light (365nm).

[0027] The pr...

Embodiment 2

[0029] Mix 69 μL of 28.7 μM double-stranded deoxyribonucleic acid (22 base pairs) aqueous solution and 40 μL of 1.5 mM bromide N,N,N-trimethyl-8-(4-(2-(4- octyloxyphenyl)-1,2-diphenylvinyl)phenoxy)octane-1-ammonium aqueous solution was mixed in a centrifuge tube, shaken for 15 minutes, then the mixture was put into a centrifuge, and the Centrifuge for 5 minutes under the centrifugal force of 6000g, discard the supernatant, and freeze-dry the obtained precipitate for 8 hours to obtain the solvent-free nucleic acid flexible fluorescent material of the present invention.

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Abstract

The invention relates to the technical field of biological materials, and mainly relates to a solvent-free nucleic acid flexible fluorescent material and a preparation method thereof. The solvent-free nucleic acid flexible fluorescent material is a compound obtained by synthesizing deoxyribonucleic acid and an aggregation-induced emission type quaternary ammonium salt compound in a water phase and combining through electrostatic interaction. The prepared solvent-free nucleic acid flexible fluorescent material can generate a strong fluorescence phenomenon under the excitation of ultraviolet light. The solvent-free nucleic acid flexible fluorescent material prepared by the invention can be applied to the related fields of biological imaging, medical research and the like.

Description

technical field [0001] The invention relates to the technical field of biological materials, and mainly relates to a solvent-free nucleic acid flexible fluorescent material and a preparation method thereof. Background technique [0002] Fluorescent materials have important application value in the fields of biochemistry and biomedicine. They can be used to label biological tissues and cells, and can also be used to track biological reaction processes. The discovery and application of green fluorescent protein provides good support for the study of many biological processes, but the application of green fluorescent protein also has its limitations. [0003] Traditional organic fluorescent materials can produce strong fluorescence in a low-concentration solution state, but if molecular aggregation occurs, their fluorescence properties will be quenched. The fluorescence quenching phenomenon caused by this kind of aggregation limits the number of labels of traditional fluoresce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H21/04C07H1/00C09K11/06
CPCC07H21/04C07H1/00C09K11/06C09K2211/1007C09K2211/1074C09K2211/1088
Inventor 吴中涛张蕾
Owner QINGDAO UNIV OF SCI & TECH