Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation of silicon nanoparticles with red fluorescence and application of silicon nanoparticles in detection of acid phosphatase

A technology of silicon nanoparticles and acid phosphatase, which is applied in the direction of fluorescence/phosphorescence, nanotechnology for materials and surface science, nanotechnology, etc., can solve the problems of restricting widespread use and high toxicity, and achieve excellent optical properties and low Effect of high toxicity and sensitivity

Active Publication Date: 2022-01-21
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these metal nanomaterials usually have high toxicity, which limits their widespread use

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation of silicon nanoparticles with red fluorescence and application of silicon nanoparticles in detection of acid phosphatase
  • Preparation of silicon nanoparticles with red fluorescence and application of silicon nanoparticles in detection of acid phosphatase
  • Preparation of silicon nanoparticles with red fluorescence and application of silicon nanoparticles in detection of acid phosphatase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Preparation of R-SiNPs

[0042] 10 mg of 2,4-diaminophenol hydrochloride was added to 10 mL of deionized water, and 1.0 mL of N-(2-aminoethyl)-3-aminopropyltrimethoxysilane ( DAMO), the above mixture was reacted at room temperature for 4 h. Then the prepared R-SiNPs solution was dialyzed in a 1000 Da (molecular weight cut-off) dialysis bag for 4 h. Finally, the dialyzed R-SiNPs solution was stored in a refrigerator at 4 °C for future use. The particle size of the obtained R-SiNPs is smaller.

Embodiment 2

[0043] Example 2 Preparation of R-SiNPs

[0044] 35 mg of 2,4-diaminophenol hydrochloride was added to 10 mL of deionized water, and 1.0 mL of N-(2-aminoethyl)-3-aminopropyltrimethoxysilane ( DAMO), the above mixture was reacted at room temperature for 2 h. Then the prepared R-SiNPs solution was dialyzed in a 1000 Da (molecular weight cut-off) dialysis bag for 4 h. Finally, the dialyzed R-SiNPs solution was stored in a refrigerator at 4 °C for future use. The particle size of the obtained R-SiNPs is uniform.

Embodiment 3

[0045] Example 3 Preparation of R-SiNPs

[0046] 5 mg of 2,4-diaminophenol hydrochloride was added to 20 mL of deionized water, and 3.0 mL of N-(2-aminoethyl)-3-aminopropyltrimethoxysilane ( DAMO), the above mixture was reacted at room temperature for 5 h. Then the prepared R-SiNPs solution was dialyzed in a 1000 Da (molecular weight cut-off) dialysis bag for 4 h. Finally, the dialyzed R-SiNPs solution was stored in a refrigerator at 4 °C for future use. The particle size distribution of the obtained R-SiNPs is not uniform.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of silicon nano-particles with red fluorescence, water-soluble silicon nano-particles (R-SiNPs) are prepared by taking N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (DAMO) as a silicon source and 2, 4-diaminophenol hydrochloride as a reducing agent, and the preparation method is simple and mild. The prepared R-SiNPs can be used for detecting acid phosphatase (ACP), the independent ACP cannot cause the fluorescence intensity change of the R-SiNPs, but KMnO4 can quench the fluorescence of the R-SiNPs, after a mixed solution of L-ascorbic acid-2-trisodium phosphate (AA2P) and ACP is added into a solution system of the R-SiNPs and the KMnO4, ascorbic acid generated by hydrolyzing AA2P by the ACP reduces KMnO4 into Mn < 2 + >, so that the fluorescence of the R-SiNPs is recovered, and along with the increase of the concentration of the ACP, the fluorescence recovery intensity of the system is continuously enhanced, so that quantitative detection and visual detection of ACP can be realized, the detection selectivity is good, and the sensitivity is high.

Description

technical field [0001] The invention belongs to the field of chemistry and chemical engineering, and in particular relates to the preparation of a silicon nanoparticle with red fluorescence and its application in detecting acid phosphatase. Background technique [0002] Acid phosphatase (ACP) is a non-specific lysosomal enzyme capable of hydrolyzing various phosphates. Generally, ACP can catalyze the hydrolysis of phosphate monoesters under acidic conditions of pH 4.0-7.0. ACP plays a very important role in the metabolism of animals, plants, bacteria and fungi. In mammals, ACP levels are closely related to pathological processes such as primary immune responses, bone remodeling, and signaling pathways. In addition, the expression of ACP in serum is high in prostate patients and diseases such as hepatitis, hyperparathyroidism, and erythrocyte lesions. At present, ACP has been identified as a marker of metastatic prostate cancer. Therefore, the detection of ACP activity is...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C09K11/59G01N21/64B82Y30/00
CPCC09K11/59G01N21/6428B82Y30/00G01N2021/6432
Inventor 韩羊霞陈佳邱洪灯
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI