3D folding ratio fluorescent microfluidic device and method for simultaneously detecting alkaline phosphatase and butyrylcholine esterase

A butyrylcholinesterase and microfluidic device technology, applied in the field of biochemical analysis and biosensors, can solve the problems of ratio sensing barriers, lack of response and detection system, etc., and achieve the effect of convenient visual detection and easy expansion

Active Publication Date: 2022-01-21
CENT SOUTH UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

However, there are still obstacles to the ratiometric sensing of multiple analytes using μPADs, such as

Method used

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  • 3D folding ratio fluorescent microfluidic device and method for simultaneously detecting alkaline phosphatase and butyrylcholine esterase
  • 3D folding ratio fluorescent microfluidic device and method for simultaneously detecting alkaline phosphatase and butyrylcholine esterase
  • 3D folding ratio fluorescent microfluidic device and method for simultaneously detecting alkaline phosphatase and butyrylcholine esterase

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preparation example Construction

[0033] Further, the method of preparing the CDS comprises placing the raw material and water in the autoclave, and the reaction is heated at 180-200 ° C for 8-15 h, and the resulting product is filtered, and the dialysis is obtained.

[0034] Further, the feedstock is an eggplant, peanut, haraffle, cucumber, kiwi, leeks, hollow dish, corn; preferred, 子; the filtration is filtered at 0.22 μm membrane, collecting filtrate; -4 days, the resulting solution was filtered after 0.22 μm membrane was filtered and dried. Finally, the obtained carbon dot was re-dissolved in water, and spare.

[0035] In one or more embodiments of the invention, a method of detecting a basic phosphatase and a butylcholine esterase, wherein the detecting method is based on the described alkaline phosphatase and dead. A 3D folding ratio fluorescence microfluidic device and / or the detection system for simultaneous detecting basic phosphatase and butoyoylide esterase.

[0036] Further, the detecting method incl...

Embodiment 1

[0043] Experimental part

[0044] 1.1 preparation of carbon dots

[0045] The cds were synthesized by hydrothermal method using water heating. Eggplant (25.0 g) and ultrapure water (15.0 mL) were placed in a polytetrafluoroethylene liner, and 10 h was heated at 190 ° C. The obtained dark product was filtered through 0.22 μm membrane, and brown filtrate was collected. Subsequently, the required solution was dialyzed with a dialysis bag (molecular interception amount of 500 da) for two days. The obtained yellow solution was filtered after 0.22 μm membrane filtered. Finally, the obtained CDS is re-dissolved in ultrapure water to reach the concentration to 10 mg ml. -1 Store to use to follow the operation.

[0046] 1.2 3D folding μPad production

[0047] Fluorescence Whatman 4 rectangular filter paper (63mm × 100 mm) is used to make three-dimensional folding μpad, fold it into a four-layer rectangular channel (ie, sample layer, reaction layer, and detection layer) (ie, sample layer, r...

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Abstract

The invention belongs to the technical field of biochemical analysis and biosensors, and relates to a 3D folding ratio fluorescent microfluidic device and method for simultaneously detecting alkaline phosphatase and butyrylcholine esterase. The device includes four layers of rectangular channels, which are arranged on filter paper, and every two layers of channels can be folded; the first layer of rectangular channel comprises a central sample area and an I-shaped path, the central sample area is located in the middle of the I-shaped path, and the two ends of the I-shaped path are circular areas; the second layer of rectangular channel adjacent to the first layer of rectangular channel comprises the I-shaped path; circular areas are arranged at the two ends of the third-layer rectangular channel and the fourth-layer rectangular channel respectively, wherein the circular areas correspond to the circular areas in the first-layer rectangular channel and the second-layer rectangular channel. The three-dimensional folding [mu]PAD has the characteristics of low cost, simplicity in operation, high efficiency, good precision, high sensitivity, good selectivity and the like, and therefore, an innovative method may be provided for the POCT field.

Description

Technical field [0001] The present invention belongs to the technical field of biochemical analysis and biosensor, and involves a 3D folding ratio fluorescence microfluidic device and method for simultaneously detecting alkaline phosphatase and butoyoylide esterase. Background technique [0002] On-site instant detection (POCT) is an important branch in vitro diagnosis, which has developed very rapidly in modern analytical chemistry and clinical testing. The POCT has an indispensable advantage that is easy to operate, setting easy, and other irreplaceable advantages, providing a possibilities for family health care, remote regional diseases. The paper base microfluidic device (μPad) focuses multiple experimental steps on a small sheet of small paper, with a simple development of simple, portable, one-time, low cost, and fast POCT. In addition, μPAD consumes a small amount of sample and reagent. In addition to commonly used wax printing, it has recently used the commercially avail...

Claims

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Application Information

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IPC IPC(8): G01N21/64B01L3/00
CPCG01N21/6428G01N21/6486B01L3/5027G01N2021/6482B01L2200/0636
Inventor 施树云朱永丰童霞王岱杰
Owner CENT SOUTH UNIV
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