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Method for determining concentration of plasma MRTX849 by high performance liquid chromatography-tandem mass spectrometry

A technology of MRTX849 and high performance liquid chromatography, which is applied in the field of high performance liquid chromatography tandem mass spectrometry to determine the concentration of plasma MRTX849, and achieves the effects of improving sensitivity, good reproducibility and preventing tailing

Pending Publication Date: 2022-01-21
BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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Problems solved by technology

However, there is no report on the quantitative detection method of MRTX849 by high performance liquid chromatography tandem mass spectrometry (HPLC-MS / MS)

Method used

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  • Method for determining concentration of plasma MRTX849 by high performance liquid chromatography-tandem mass spectrometry
  • Method for determining concentration of plasma MRTX849 by high performance liquid chromatography-tandem mass spectrometry
  • Method for determining concentration of plasma MRTX849 by high performance liquid chromatography-tandem mass spectrometry

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Embodiment Construction

[0031] The present invention will be further described in detail below in conjunction with the accompanying drawings, so that those skilled in the art can implement it with reference to the description.

[0032] It should be understood that terms such as "having", "comprising" and "including" as used herein do not entail the presence or addition of one or more other elements or combinations thereof.

[0033] It should be noted that the experimental methods described in the following embodiments, unless otherwise specified, are conventional methods, and the reagents and materials, unless otherwise specified, can be obtained from commercial sources.

[0034] 1 solution preparation

[0035] The volumes and weights used to prepare solutions can be adjusted proportionally, and all solutions are stored at room temperature unless otherwise stated.

[0036] 1.1 Preparation of mobile phase solution

[0037] Organic phase (A): acetonitrile containing formic acid in a mass fraction of 0....

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Abstract

The invention discloses a method for measuring the concentration of plasma MRTX849 through high performance liquid chromatography-tandem mass spectrometry, which comprises the following steps: dissolving MRTX849 by using methanol to prepare a standard curve working solution with multiple concentrations, and preparing an internal standard substance working solution by using methanol, wherein the linear range of the standard curve is 0.05-200ng / mL; then precisely sucking 50 [mu] L of to-be-detected plasma, adding an internal standard substance working solution, carrying out vortex and centrifugation, taking supernate, carrying out HPLC-MS / MS quantitative analysis, and reading the concentration of MRTX849 on a standard curve. The invention lays a technical foundation for subsequent clinical conventional treatment drug monitoring.

Description

technical field [0001] The present invention relates to methods for detecting drug concentrations. More specifically, the present invention relates to a method for measuring plasma MRTX849 concentration by high performance liquid chromatography tandem mass spectrometry. Background technique [0002] In recent years, with the widespread clinical application of anti-tumor molecular targeted drugs, epidermal growth factor receptor (EGFR), anaplastic lymphoma kinase (ALK) rearrangement, vascular endothelial growth factor receptor (VEGFR), etc. Tyrosine kinase inhibitors (TKIs) have significantly improved the benefit-risk ratio in patients with advanced cancer. The KRAS gene, located on chromosome 12, is a proto-oncogene of the RAS family and an important "switch" in the intracellular signal transduction pathway. Once it is turned on, it activates multiple division and proliferation factors, including c-Raf and PI3K. Under normal circumstances, KRAS will bind to GTP, cut off t...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/045
Inventor 杜萍安卓玲王国永钟久昌
Owner BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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