Application method of nanoparticle-targeted Piezo1 protein in acute lung injury

Abstract

The invention discloses an application method of a nanoparticle-targeted Piezo1 protein in acute lung injury. The method comprises the following steps: S1, preparing materials; S2, treating the materials; S3, performing RT-PCR detection on gene expression; S4, detecting protein expression through Western blotting; S5, detecting the level of TNF-alpha and IL-1beta inflammatory factors by ELISA; S6, determining the LDH level of the serum; S7, performing HE dyeing on the lung tissue of the mouse and performing pathological scoring; S8, detecting Piezo1 expression of the lung tissue of the mouse by an immunohistochemical method and immunofluorescence; S9, detecting the total protein and the total cell number in the mouse BALF; S10, determining the wet / dry mass ratio of the lung tissue; S11, carrying out Tunel staining to detect lung tissue cell apoptosis; S12, preparing a GsMTx4 coated BSA / chitosan nano particle carrier (NC-GsMTx4); S13, performing characterization and the biological action of the NC-GsMTx4; S14, detecting the biocompatibility of the NC-GsMTx4; S15, realizing the intervention effect of aerosol inhalation of NC-GsMTx4 on an acute lung injury (ALI) mouse induced by LPS; and S16, performing statistics. The method is clear in target spot, accurate in intervention, quick in effect and small in side effect.

Description

technical field [0001] The invention belongs to the field of medical technology, and in particular relates to an application method for targeting Piezol protein based on nanoparticles in acute lung injury. Background technique [0002] Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are critical respiratory diseases characterized by diffuse alveolar damage and clinical manifestations of respiratory distress. Such fatal diseases are the main causes of morbidity and mortality in ICU. Epidemiological surveys show that there are nearly 196,000 ALI patients in the United States each year, accounting for 3.6 million hospital days, resulting in 75,000 deaths, and ARDS mortality is still as high as 40%. Therefore, it is the main clinical problem to study its pathogenesis in depth and find a treatment method for the underlying pathological mechanism of ALI / ARDS. [0003] At present, the pathogenesis of ALI is still unclear. It is believed that the pathogenesis...

AI Technical Summary

Problems solved by technology

The researchers found that NC-encapsulated curcumin, resveratrol, simvastatin, dexamethasone and other drugs overcome the shortcomings of poor water solubility and low stability of traditional drugs, and can effectively alleviate the progress of ALI

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