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Method for regulating, controlling and reconstructing collagen molecular weight distribution

A molecular weight distribution and collagen technology, which is applied in the field of regulating and controlling the reconstruction of collagen molecular weight distribution, can solve the problems of large molecular weight, solubility, poor tissue permeability, etc., and achieves the effects of convenient preparation, simple operation and low cost.

Active Publication Date: 2022-01-28
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Collagen prepared by existing methods is a narrowly dispersed biomacromolecular material, which has unique functions such as moisturizing, whitening, nutrition, repairing, and tissue induction. poor, which limits its application

Method used

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  • Method for regulating, controlling and reconstructing collagen molecular weight distribution
  • Method for regulating, controlling and reconstructing collagen molecular weight distribution
  • Method for regulating, controlling and reconstructing collagen molecular weight distribution

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1: a method for regulating and controlling the molecular weight distribution of reconstructed collagen, comprising the following steps:

[0028] S1. Desugar and degreasing treatment: first decontaminate, depilate, wash the animal skin, freeze and section it, and then desugar and degrease it;

[0029] S2. Swelling: Put 1000g of animal skin after desugar and degreasing treatment into the reaction vessel, add 10L of hydrochloric acid with a concentration of 0.1mol / L and adjust the pH value of the solution to 1.5, control the temperature at 4°C, and the stirring speed at 300± Stir at 10rpm to fully swell the animal tissue for 0.5h;

[0030] S3. Enzyme digestion: add 0.5 g of papain to the swollen animal tissue and react for 6 hours;

[0031] S4. Viscosity adjustment: control the temperature at 34°C, stir at 60±60rpm, and stir until the solution digested by the enzyme completely dissolves and loses viscosity;

[0032] S5. Adjust molecular weight distribution:

...

Embodiment 2

[0037] Embodiment 2: a kind of method of regulating and controlling the molecular weight distribution of rebuilding collagen, comprises the following steps:

[0038] S1. Desugar and degreasing treatment: decontaminate and clean the animal tendon first, then freeze and section it, and then desugar and degrease it;

[0039]S2. Swelling: put 1000g of animal skin after desugar and degreasing treatment into the reaction vessel, add 20L of phosphoric acid with a concentration of 0.1mol / L and adjust the pH value of the solution to 3, control the temperature at 30°C, and the stirring speed at 300± Stir at 10rpm to fully swell the animal tissue for 2 hours;

[0040] S3. Enzyme digestion: add pepsin 2g to the swollen animal tissue and react for 48 hours;

[0041] S4. Viscosity adjustment: control the temperature at 42°C, stir at 60±60rpm, and stir until the solution digested by the enzyme completely dissolves and loses viscosity;

[0042] S5. Adjust molecular weight distribution:

[...

Embodiment 3

[0047] Embodiment 3: a kind of method of regulating and controlling the molecular weight distribution of rebuilding collagen, comprises the following steps:

[0048] S1. Desugar and degreasing treatment: decontaminate and clean the animal bones first, freeze and section them, and then desugar and degrease them;

[0049] S2. Swelling: put 1000g of animal skin after desugar and degreasing treatment into the reaction vessel, add 10L of acetic acid with a concentration of 0.25mol / L and adjust the pH value of the solution to 2, control the temperature at 10°C, and the stirring speed at 300± Stir at 10rpm to fully swell the animal tissue for 5 hours;

[0050] S3. Enzyme digestion: add 20 g of bromelain to the swollen animal tissue and react for 12 hours;

[0051] S4. Viscosity adjustment: control temperature at 36° C., stirring speed is 60 ± 60 rpm, stir until the solution after enzyme digestion completely dissolves and loses viscosity;

[0052] S5. Adjust molecular weight distrib...

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Abstract

The invention discloses a method for regulating, controlling and reconstructing collagen molecular weight distribution. According to the method, animal tissues are subjected to desugaring and degreasing treatment, swelling, enzyme digestion, viscosity adjustment and molecular weight distribution adjustment, and finally, the obtained collagen has a triple helix structure and polydisperse molecular weight distribution. According to the method disclosed by the invention, the collagen raw material has the characteristics of collagen activity and polydispersity distribution of molecular weight, retains good biological functions of the collagen, is the fourth collagen different from macromolecular collagen, gelatin and hydrolyzed collagen, and expands the application of collagen. The method is simple to operate, convenient for preparation, low in cost and suitable for industrial large-scale production.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for regulating and controlling the molecular weight distribution of reconstructed collagen. Background technique [0002] Collagen is the main component of the extracellular matrix of animal connective tissue. It exists in various tissues and organs of animals, especially in skin, bones, tendons and pericardium. So far, at least 20 genetically different collagen molecules have been found in animals. Collagen in vivo (mainly fibrous collagen molecules) is about 300 nm in length and 1.5 nm in diameter, and has a unique unique structure composed of 3 polypeptide chains. The interrupted right-handed triple helix structure has structural support and special biological functions in organisms. [0003] Collagen and its derivatives derived from animal tissues can obtain products with different physicochemical and biological properties due to different prepara...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12P21/06
CPCC07K14/78C12P21/06
Inventor 蒋波李霞王宁馨罗振宇郭纪彤尹越朱凌旋
Owner SICHUAN UNIV
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