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Pseudo-ginseng quality detection method based on spectrum-activity relationship

A quality detection method and detection method technology, which are applied in the field of pharmaceutical inventions, can solve the problems of misjudgment of characteristic peaks, ratio of characteristic peaks of unconfirmed characteristic peaks relative to retention time, difficulty in determining the qualified range of medicinal materials, etc. The effect of accuracy

Active Publication Date: 2022-02-08
贵州中医药大学
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Documents 1 and 2 were also developed by the team of the present invention, and the following technical problems were found during use: 1) Only the characteristic peaks of medicinal materials were confirmed in the spectrum-effect relationship, and the relative retention time of the characteristic peaks and the peak proportion of the characteristic peaks were not confirmed , resulting in that the characteristic peaks cannot be directly found or determined in the subsequent use, which may easily lead to misjudgment of the characteristic peaks in the detection process and affect the accuracy and directness of the detection method; 2) only the characteristics of the medicinal materials are found in the spectrum-effect relationship Peak, did not confirm the specific range of the characteristic peak peak area, it is difficult to determine the qualified range of medicinal materials
In fact, traditional Chinese medicine is a multi-component complex system, and its curative effect is the result of multi-component, multi-target, and multi-channel synergistic effects. Simple quantification of chemical components is difficult to comprehensively evaluate the quality of traditional Chinese medicine.

Method used

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  • Pseudo-ginseng quality detection method based on spectrum-activity relationship
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  • Pseudo-ginseng quality detection method based on spectrum-activity relationship

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Fingerprint

[0051] Preparation of the mixed reference substance solution: Accurately weigh 10 mg each of the reference substances notoginsenoside R1, ginsenoside Rg1, ginsenoside Rb1, ginsenoside Re, ginsenoside Rd, ginsenoside RH1 and ginsenoside Rg2, respectively, add methanol solution to constant volume In a 10mL volumetric flask, the standard solution is obtained;

[0052] Preparation of the test solution: Accurately weigh 15.0 g of Panax notoginseng powder, add 7.7 times the amount of 66% ethanol to reflux for extraction for 1.5 hours, extract twice, and combine the filtrates; dilute the filtrate to a 200mL volumetric flask, and centrifuge at 13000r / min for 10min , through a 0.22μm microporous membrane to obtain the test solution.

[0053] Chromatographic conditions and system adaptability test: Chromatographic column: ACQUITY UPLC BEH Shield C18, chromatographic column specification: 2.1×100mm, 1.7μm; mobile phase: 0.1% phosphoric acid water as mobile phase A, ...

Embodiment 2

[0083] Fingerprint

[0084] Preparation of the mixed reference substance solution: Accurately weigh 10 mg each of the reference substances notoginsenoside R1, ginsenoside Rg1, ginsenoside Rb1, ginsenoside Re, ginsenoside Rd, ginsenoside RH1 and ginsenoside Rg2, respectively, add methanol solution to constant volume In a 10mL volumetric flask, the standard solution is obtained;

[0085] Preparation of the test solution: Accurately weigh 15.0 g of Panax notoginseng powder, add 7 times the amount of 60% ethanol to reflux for extraction for 1 hour, extract twice, and combine the filtrates; dilute the filtrate to a 200mL volumetric flask, and centrifuge at 13000r / min for 5min , through a 0.22 μm microporous membrane to obtain the test solution;

[0086] Chromatographic conditions and system adaptability test: Chromatographic column: ACQUITY UPLC BEH Shield C18, chromatographic column specification: 2.1×100mm, 1.7μm; mobile phase: 0.1% phosphoric acid water as mobile phase A, mobil...

Embodiment 3

[0090] Fingerprint

[0091] Preparation of the mixed reference substance solution: Accurately weigh 10 mg each of the reference substances notoginsenoside R1, ginsenoside Rg1, ginsenoside Rb1, ginsenoside Re, ginsenoside Rd, ginsenoside RH1 and ginsenoside Rg2, respectively, add methanol solution to constant volume In a 10mL volumetric flask, the standard solution is obtained;

[0092] Preparation of the test solution: Accurately weigh 15.0 g of Panax notoginseng powder, add 8 times the amount of 70% ethanol to reflux for extraction for 2 hours, extract twice, and combine the filtrates; dilute the filtrate to a 200mL volumetric flask, and centrifuge at 13000r / min for 15min , through a 0.22 μm microporous membrane to obtain the test solution;

[0093] Chromatographic conditions and system adaptability test: Chromatographic column: ACQUITY UPLC BEH Shield C18, chromatographic column specification: 2.1×100mm, 1.7μm; mobile phase: 0.1% phosphoric acid water as mobile phase A, mob...

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Abstract

The invention discloses a pseudo-ginseng fingerprint spectrum and quality detection method, which comprises the following steps: firstly, establishing a pseudo-ginseng fingerprint spectrum, establishing a spectrum-effect relationship by utilizing the pseudo-ginseng fingerprint spectrum and a blood activating effect, and determining a characteristic peak by adopting a peak proportion and relative retention time so as to establish a pseudo-ginseng quality evaluation standard. The method is accurate and rapid; the correlation between the chemical components in the pseudo-ginseng and the blood activating effect is clarified; the pharmacological action of the pseudo-ginseng on myocardial ischemia and blood stasis is discussed by researching the efficacy of the pseudo-ginseng extract, so that a scientific basis is provided for subsequent deep research, and an effective method is provided for judging the quality of the pseudo-ginseng.

Description

technical field [0001] The invention relates to the field of medical inventions, in particular to a method for detecting the quality of Panax notoginseng based on spectrum-effect relationship. Background technique [0002] Panax notoginseng, the name of traditional Chinese medicine. It is the dry root of Panax notoginseng (Burk.) F.H.Chen. It has the effects of removing blood stasis and stopping bleeding, promoting blood circulation and relieving pain. Indications for various bleeding syndromes, blood stasis, swelling and pain, bruises, etc. belong to stagnation of blood stasis. Pharmacological effects include: 1. Effects on the blood system: it can significantly shorten the bleeding and coagulation time, and has anti-platelet aggregation and thrombolytic effects; it can promote the proliferation of multifunctional hematopoietic stem cells, and has hematopoietic effects. 2. Effects on cardiovascular and cerebrovascular systems: Panax notoginseng saponins can fight against...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/86G01N30/88
CPCG01N30/8686G01N30/8696G01N30/88G01N2030/8809
Inventor 徐剑张永萍张宁刘耀刘杰
Owner 贵州中医药大学
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