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Method for detecting ciprofloxacin by using LSPR colorimetric aptamer sensor based on AuNPs

An aptamer sensor, ciprofloxacin technology, is applied in the detection field of fluoroquinolone antibiotics, and achieves the effects of strong specificity, accurate experimental results and improved sensitivity

Active Publication Date: 2022-02-11
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no AuNPs-based LSPR colorimetric aptasensor and a simple, rapid, highly sensitive and visual detection method for ciprofloxacin using the sensor

Method used

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  • Method for detecting ciprofloxacin by using LSPR colorimetric aptamer sensor based on AuNPs
  • Method for detecting ciprofloxacin by using LSPR colorimetric aptamer sensor based on AuNPs
  • Method for detecting ciprofloxacin by using LSPR colorimetric aptamer sensor based on AuNPs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Preparation of AuNPs

[0033] Measure 100mL of HAuCl 4 (0.2mmol / L) in a round-bottomed flask, heated and stirred on a rotary evaporator (60rpm) until boiling (100°C); quickly added 3.5mL of 1% trisodium citrate, the light yellow gradually disappeared, when the solution was wine Continue heating for 10 minutes when it is red, stop heating, stir to room temperature; filter the solution with a 0.45 μm micropore filter to prepare AuNPs for use. AuNPs were centrifuged at 4°C or diluted to an absorbance of 0.5 at 525 nm, and stored at 4°C.

Embodiment 2

[0035] 1. Determination of aptamer concentration

[0036] Take 20-200nmol / L aptamer in Tris-HCl (10mmol / L, pH=7.4, containing 1mmol / L K + and 1mmol / LMg 2+ ) buffer solution, then add AuNPs (the volume of AuNPs with an absorbance of 0.5 at 525nm accounts for 1 / 2 of the volume of the reaction system) and target CIP (0 and 10μM) to form a reaction system, and stand at room temperature (25°C) for 50min After that, add the same volume of sensitizer (containing 1mmol / LHAuCl 4and 2mmol / LNHOH·HCl in 10mmol / LpH=4 citric acid buffer solution) to promote the growth of AuNPs. The effect of different concentrations of aptamers on the red shift of the LSPR peak of AuNPs in the system solution is shown in figure 2 . (λ displacement; λ displacement = λ f- lambda i ; lambda f is the peak wavelength at CIP 10μM, λ i is the peak wavelength at CIP 0 μM. )Depend on figure 2 It can be seen that with the increase of the aptamer concentration, the wavelength difference of the peak red shi...

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Abstract

The invention discloses a method for detecting ciprofloxacin by using an LSPR colorimetric aptamer sensor based on AuNPs, and belongs to the field of detection of fluoroquinolone antibiotics. The method comprises the following steps: sequentially adding an aptamer, AuNPs and ciprofloxacin into a Tris-HCl-containing buffer solution to form a reaction system, and carrying out standing reaction on the reaction system at 20-45 DEG C for 20-90 minutes; then adding sensitizers HAuCl4 and NHOH.HCl, specifically binding the aptamer with ciprofloxacin to lose the protection effect on AuNPs, promoting the growth of AuNPs in the reaction system and the change of the color of the solution by the sensitizers, and analyzing the concentration of ciprofloxacin according to the red shift degree of an LSPR wave crest, thereby realizing simple, rapid, high-sensitivity and visual detection of ciprofloxacin.

Description

technical field [0001] The invention belongs to the detection field of fluoroquinolone antibiotics (FQs), and more specifically relates to an AuNPs-based LSPR colorimetric aptamer sensor and a method for rapidly detecting ciprofloxacin (CIP) using the sensor. Background technique [0002] Fluoroquinolones are widely used in human and veterinary medicine to treat various infectious diseases caused by Gram-negative and Gram-positive bacteria. Due to uncontrolled use, FQs can be detected in different substances such as food and surface water. Ciprofloxactin (CIP) is a third-generation fluoroquinolone antibiotic widely used in the healthcare and agricultural industries for the treatment of respiratory and gastrointestinal infections in humans and livestock. Although CIP has a significant therapeutic effect in medicine, it can remain in meat and milk, causing some adverse side effects and diseases, such as skin infection, respiratory infection, chronic bacterial prostatitis, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/52
CPCG01N33/5308G01N33/52
Inventor 李胎花王婷李滨汐吕新月王雪金龙
Owner NANJING FORESTRY UNIV
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