Preparation of caffeic acid or caffeic acid derivative metal chelate nanoparticles and application thereof

A metal chelate and nanoparticle technology, applied in the field of medicine, can solve problems such as inability to achieve long-term treatment, and achieve the effects of promoting wound healing, reducing cell calcification, and reversing cell apoptosis

Pending Publication Date: 2022-02-15
THE SEVENTH AFFILIATED HOSPITAL SUN YAT SEN UNIV SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the early degeneration of the intervertebral disc, the current artificial intervention is to promote the secretion of extracellular matrix and slow down the apoptosis of the intervertebral disc cells. Effect of long-term treatment

Method used

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  • Preparation of caffeic acid or caffeic acid derivative metal chelate nanoparticles and application thereof
  • Preparation of caffeic acid or caffeic acid derivative metal chelate nanoparticles and application thereof
  • Preparation of caffeic acid or caffeic acid derivative metal chelate nanoparticles and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] The present embodiment provides a preparation scheme for the preparation of magnesium caffeate, and the specific steps are as follows:

[0029] a Dissolve 90mg of caffeic acid in a mixed solution of 50ml of ethanol and water (volume ratio 1:1), stir until completely dissolved, and mark it as solution A.

[0030] b Dissolve 105 mg of magnesium chloride hexahydrate in the above solution A, stir until completely dissolved, and mark it as solution B.

[0031] c Adjust the pH value of solution B to 10.1, and mark it as solution C.

[0032] d Solution C was reacted in a water bath at 4°C for 20 minutes, then washed twice with deionized water and twice with alcohol, and freeze-dried by a freeze dryer to obtain a dark brown product, which was named magnesium caffeate nanoparticles.

Embodiment 2

[0034] The present embodiment provides a preparation scheme for the preparation of calcium caffeate, and the specific steps are as follows:

[0035] a Dissolve 90mg of caffeic acid in a mixed solution of 50ml of ethanol and water (volume ratio 1:1), stir until completely dissolved, and mark it as solution A.

[0036] b Dissolve 165mg of anhydrous calcium chloride in the above solution A, stir until completely dissolved, and mark it as solution B.

[0037] c Adjust the pH value of solution B to 10.1, and mark it as solution C.

[0038] d Solution C was reacted in a water bath at 4°C for 20 minutes, then washed twice with deionized water and twice with alcohol, and freeze-dried by a freeze dryer to obtain a dark brown product, which was named as calcium caffeate nanoparticles.

Embodiment 3

[0040] The present embodiment provides a preparation scheme for the preparation of iron caffeate, and the specific steps are as follows:

[0041] a Dissolve 90mg of caffeic acid in a mixed solution of 50ml of ethanol and water (volume ratio 1:1), stir until completely dissolved, and mark it as solution A.

[0042] b Dissolve 165 mg of anhydrous ferric chloride in the above solution A, stir until completely dissolved, and mark it as solution B.

[0043] c Adjust the pH value of solution B to 10.1, and mark it as solution C.

[0044] d Solution C was reacted in a water bath at 4°C for 20 minutes, then washed twice with deionized water and twice with alcohol, and freeze-dried by a freeze dryer to obtain a dark brown product, which was named iron caffeate nanoparticles.

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PUM

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Abstract

The invention provides a preparation method of caffeic acid or caffeic acid derivative metal chelate nanoparticles, which comprises the following steps: S1, dissolving caffeic acid or caffeic acid derivative and metal chloride in a water phase solution, stirring until the caffeic acid or caffeic acid derivative and metal chloride are completely dissolved, and adjusting the pH value of the solution to be neutral or alkaline; and S2, reacting the solution obtained in the step S1 for a period of time, washing, and freeze-drying the obtained reaction product to obtain black brown powder, namely the caffeic acid metal chelate nanoparticles. The caffeic acid or the derivative of the caffeic acid and metal ions are chelated to obtain the metal chelate nanoparticles of the caffeic acid or the derivative of the caffeic acid, and the nanoparticles have the effect of removing free and intracellular active oxygen and can reverse oxidative stress induced cell apoptosis. The preparation method is simple in process and easy to operate.

Description

technical field [0001] The invention relates to the field of medicines, in particular to the preparation and application of metal chelate nanoparticles of caffeic acid or derivatives thereof. Background technique [0002] At present, it is generally believed that aging is the result of the joint action of various internal and external factors that cause cell damage and death. With the development of free radical biomedical research, people have gradually expanded the research on free radical aging to more fields, such as various diseases associated with aging, such as senile dementia, arteriosclerosis and diabetes, which are closely related to free radical damage. relation. Subsequent studies gradually penetrated to the cellular level, proving that apoptosis and necrosis associated with many diseases are the result of free radical damage. [0003] According to the free radical theory of aging, the decline of tissue and organ function is closely related to the oxidative str...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/192A61K33/06A61K33/26A61K33/34A61K33/30A61K33/24A61K9/51A61K47/52A61P39/06A61P19/08
CPCA61K31/192A61K33/06A61K33/26A61K33/34A61K33/30A61K33/24A61K9/5115A61P39/06A61P19/08A61K2300/00
Inventor 魏富鑫赵静杨家明曾宝珠刘鹏汪强
Owner THE SEVENTH AFFILIATED HOSPITAL SUN YAT SEN UNIV SHENZHEN
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