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Preparation method of cayratia japonica total flavonoids

A technology of black berry and total flavonoids, which is applied in the field of separation and purification, can solve the problem of low extraction and purification purity of black berry total flavonoids, and achieve the effect of increasing the content

Active Publication Date: 2022-02-25
NANJING HOSPITAL OF T C M +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem of low purity in the extraction and purification of total flavonoids of black berry, it is very important to find a preparation process with higher purity of total flavonoids of black berry

Method used

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  • Preparation method of cayratia japonica total flavonoids
  • Preparation method of cayratia japonica total flavonoids
  • Preparation method of cayratia japonica total flavonoids

Examples

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Embodiment 1

[0030] A method for preparing total flavonoids of black berry, comprising the following steps: washing, drying, and pulverizing 20.0 kg of black berry medicinal material through a 100-mesh sieve for use; 240L, extract twice, filter and combine the filtrates, recover under reduced pressure until the crude drug concentration is 1g / ml, centrifuge at 4000r / min at low speed for 20min, and take the supernatant. Add an appropriate amount of hydrochloric acid to the supernatant to adjust the pH to 4.0, the crude drug concentration is 0.5g / ml, the AB-8 macroporous resin column diameter-to-height ratio is 1:6, the sample volume is 5BV, and the sample flow rate is 5BV / h. After adsorption, wash with 10BV of purified water at a flow rate of 5BV / h, then elute with 3BV of ethanol with a volume fraction of 50% at a flow rate of 5BV / h, collect the eluate, and concentrate to dryness under reduced pressure , to obtain the total flavonoids extract of black raspberries.

Embodiment 2

[0032] A method for preparing total flavonoids of black berry, comprising the following steps: washing, drying, and pulverizing 20.0 kg of black berry medicinal material through a 100-mesh sieve for use; 240L, extract twice, filter and combine the filtrates, recover under reduced pressure until the crude drug concentration is 1g / ml, centrifuge at 4000r / min at low speed for 20min, and take the supernatant. Add an appropriate amount of hydrochloric acid to the supernatant to adjust the pH to 7.0, the crude drug concentration is 0.5g / ml, the diameter-to-height ratio of the AB-8 macroporous resin column is 1:6, the sample volume is 5BV, and the sample flow rate is 2BV / h. After adsorption, wash with 3BV of purified water at a flow rate of 5BV / h, then elute with 30% ethanol 3BV at a flow rate of 5BV / h, collect the eluate, and concentrate to dryness under reduced pressure , to obtain the total flavonoids extract of black raspberries.

Embodiment 3

[0034] A method for preparing total flavonoids of black berry, comprising the following steps: washing, drying, and pulverizing 20.0 kg of black berry medicinal material through a 100-mesh sieve for use; 240L, extract twice, filter and combine the filtrates, recover under reduced pressure until the crude drug concentration is 1g / ml, centrifuge at 4000r / min at low speed for 20min, and take the supernatant. Add an appropriate amount of hydrochloric acid to the supernatant to adjust the pH to 6.0, the crude drug concentration is 0.5g / ml, the AB-8 macroporous resin column diameter-to-height ratio is 1:6, the sample volume is 5BV, and the sample flow rate is 6BV / h. After adsorption, wash with 15BV of purified water at a flow rate of 5BV / h, then elute with 60% ethanol 6BV at a flow rate of 5BV / h, collect the eluate, and concentrate to dryness under reduced pressure , to obtain the total flavonoids extract of black raspberries.

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Abstract

The invention provides a preparation method of cayratia japonica total flavonoids, and relates to the technical field of separation and purification. The preparation method comprises the following steps: (1) preprocessing a cayratia japonica medicinal material: cleaning, drying, crushing and sieving the cayratia japonica medicinal material to obtain cayratia japonica medicinal material decoction pieces for later use; (2) preparing a cayratia japonica concentrated solution: drying the cayratia japonica medicinal material decoction pieces, carrying out ethanol reflux extraction, carrying out vacuum concentration, centrifuging, and taking supernatant, so as to obtain the cayratia japonica concentrated solution; (3) packing and sample loading: pre-treating AB-8 macroporous adsorption resin, packing into a column, adjusting the pH value of the cayratia japonica concentrated solution, loading into the sample, and dynamically adsorbing; and (4) performing impurity removal and elution: washing an adsorption column with water, eluting with ethanol, collecting ethanol eluate, and drying under reduced pressure to obtain cayratia japonica total flavonoid extract. By optimizing various parameters in the preparation method, the content of flavone in the cayratia japonica can be effectively increased.

Description

technical field [0001] The invention relates to the technical field of separation and purification, in particular to a preparation method of total flavonoids of black currant. Background technique [0002] Blackberry is the whole herb of Cayratia japonica (Thunb.) Gagnep., a plant of the genus Blackberry in the grape family. It has the effects of clearing away heat and dampness, detoxifying and reducing swelling. Pain, jaundice, etc. Blackberry paste is a well-known preparation in Nanjing Hospital of Traditional Chinese Medicine. It has been used clinically for more than 30 years. It has been clinically shown that it has good curative effects on perianal abscess, mastitis, parotitis, gouty arthritis, rheumatoid arthritis and low back pain. curative effect. [0003] At present, many documents have carried out research on the extraction process of black berry, for example, literature: Zhao Xuelong, etc., the current situation and analysis of pharmacy and clinical research of...

Claims

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Application Information

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IPC IPC(8): A61K36/87
CPCA61K36/87A61K2236/13A61K2236/15A61K2236/17A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55
Inventor 赵学龙郑雪平包贝华张丽郭华曹亮亮
Owner NANJING HOSPITAL OF T C M