Unlock instant, AI-driven research and patent intelligence for your innovation.

Composite demineralized bone matrix composition containing bone mineral component and method for producing same

A technology of composite demineralized bone and bone inorganic matter, applied in bone implants, tissue regeneration, medical science and other directions, can solve the problems of undisclosed methods, loss, etc., to achieve biocompatibility, excellent bone formation effect, Excellent effect of osteoconductivity and osteoinductivity

Active Publication Date: 2022-02-25
爱恩斯生物科技(昆山)有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As the prior art for preparing bone inorganic matter, there is no disclosure of methods for obtaining these bioactive substances released from demineralized bone together with bone inorganic matter
Moreover, in the prior art, after the bone inorganic matter precipitation process, the refined bone inorganic matter powder is obtained by carrying out the refining and washing process using distilled water, and the biologically active substance will be dissolved again during several times of washing with distilled water loss in distilled water

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composite demineralized bone matrix composition containing bone mineral component and method for producing same
  • Composite demineralized bone matrix composition containing bone mineral component and method for producing same
  • Composite demineralized bone matrix composition containing bone mineral component and method for producing same

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0038] The present invention relates to the preparation method of compound demineralized bone matrix (DBM) complex composition by one-step method, it is characterized in that, comprises the following steps:

[0039] Decalcification step, using an acid solution to decalcify the bone tissue;

[0040] The bone inorganic matter precipitation step is to neutralize the decalcification-treated decalcification solution with an alkaline solution to precipitate bone inorganic matter;

[0041] BMP-2 extraction step, centrifuging the above precipitated solution, adding an acid solution to the precipitate to extract BMP-2; and

[0042] In the preparation step of the composite decalcified bone composition, the solution extracted from the above-mentioned BMP-2 is neutralized with an alkaline solution, and the decalcified bone, bone inorganic matter and BMP-2 are separated out.

[0043] Through the examples of the present invention, the composite decalcified bone composition is prepared by a...

Embodiment approach

[0140] Hereinafter, the present invention will be described more specifically by way of examples. However, the scope of the present invention is not limited to the following examples, and those skilled in the art can understand that various variations, modifications, or applications can be made without departing from the technical idea derived from the idea described in the claims.

Embodiment 1

[0142] Example 1. Obtaining composite demineralized bone composition by one-step method

[0143] With isopropanol (2-Propanol for analysis ACS, ISO, Reag. Ph Eur, Merck) washed the cortical bone, and then performed a pretreatment process of washing with sterilized distilled water.

[0144] Thereafter, 1 g of cortical bone was decalcified at room temperature for 4 hours using 10 to 50 ml of 0.5 to 1.5 normality (N) hydrochloric acid solution (Hydrochloric acid 35.0-37.0%, JUNSEI CHEMICAL CO., LTD.). After the above-mentioned decalcification is completed, use 0.5 to 4.0 M sodium hydroxide (NaOH, Sodiumhydroxide, Merck) solution in the mixed solution composed of supernatant and sediment to induce the precipitation of bone inorganic matter in the mixed solution, so that it can be combined with decalcified bone Precipitate together. Centrifuge the mixed solution that has completed precipitation and precipitation at 1000 to 8000 rpm for 10 to 30 minutes, and remove the supernatan...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
complex viscosityaaaaaaaaaa
complex viscosityaaaaaaaaaa
Login to View More

Abstract

The present invention relates to a method for producing a composite demineralized bone matrix composition using a one-step process. The composite demineralized bone matrix composition, according to the present invention, is produced from biologically-derived bone. In addition, the composite demineralized bone matrix composition produced by the present invention contains bone mineral according to the original composition proportion in the bone, and may provide a bone mineral content condition which is closest to that in an environment in which in vivo bone formation occurs. In addition, the composition contains a bone morphogenetic protein (BMP-2), and thus enables a stable and excellent bone formation effect to be derived.

Description

technical field [0001] The invention relates to a composite decalcified bone composition containing bone inorganic matter and decalcified bone and a preparation method thereof. In addition, the present invention relates to a compound dosage type composition for bone grafting containing bone inorganic substance, demineralized bone and polymer excipient. [0002] Specifically, the present invention is a composite inorganic substance-decalcified bone (hereinafter referred to as "decalcified bone") obtained by mixing bone inorganic substance obtained from biological bone (bone) with a composition ratio similar to that of the original bone, and decalcified bone. Composite decalcified bone composition, a one-step method for preparing it, and a biosource polymer excipient for maintaining the dosage form of the composite decalcified bone composition. Background technique [0003] With the aging of the population, the diversification of industrial society, the increase in the rate o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/20A61L27/50A61L27/54A61F2/28
CPCA61L27/3608A61L27/365A61L27/3687A61L27/20A61L27/50A61L27/54A61F2/28A61F2/30942A61L2430/02A61L2430/40A61F2002/2835A61F2002/3006C08L5/08C08L1/286A61F2002/2817A61F2002/4649A61L27/44A61L27/3691A61L27/52A61L27/58A61L27/26A61L27/40A61L2300/412
Inventor 李誾成李基源金炯九李焕铁
Owner 爱恩斯生物科技(昆山)有限公司