Application of glehnia littoralis lactone and SIRT1 up-regulating agent in preparation of medicine for promoting wound healing of inflammatory diseases

A Coral lactone, inflammatory disease technology, applied in skin diseases, drug combinations, active ingredients of heterocyclic compounds, etc., can solve problems such as drugs for wound healing of no inflammatory diseases, and achieve the promotion of proliferation and proliferation. , the effect of promoting proliferation

Pending Publication Date: 2022-03-01
YUEYANG INTEGRATED TRADITIONAL CHINESE & WESTERN MEDICINE HOSPITAL SHANGHAI UNIV OF CHINESE TRADITIONAL MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there are no related reports on the preparation of drugs that promote wound healing in inflammatory diseases by using pectinolide and SIRT1 up-regulators

Method used

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  • Application of glehnia littoralis lactone and SIRT1 up-regulating agent in preparation of medicine for promoting wound healing of inflammatory diseases
  • Application of glehnia littoralis lactone and SIRT1 up-regulating agent in preparation of medicine for promoting wound healing of inflammatory diseases
  • Application of glehnia littoralis lactone and SIRT1 up-regulating agent in preparation of medicine for promoting wound healing of inflammatory diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 The composition of Shengji Huayu formula, sarcolide, promotes wound healing in mice with diabetic ulcers

[0046] 1 Materials and methods

[0047] 1.1 Experimental materials

[0048] 1.1.1 Experimental animals

[0049] Animal source: SPF grade C57BL / 6J mice, 8-9 weeks old, weighing 25-30g, male, purchased from Shanghai Xipuer-Bikay Experimental Animal Co., Ltd. (license number: SCXK (Shanghai) 2018-0006; certificate of conformity Number: 20180006016496).

[0050] Raising conditions for mice: 40% high-fat diet, free access to water.

[0051] Breeding environment: SPF grade, temperature (23±2)°C, relative humidity (40±10)%.

[0052] 1.1.2 Acquisition of pectinolide

[0053] The experimental process is as follows figure 1 As shown, the components of Shengji Huayu Formula are provided by Shanghai Wanshicheng National Pharmaceutical Products Co., Ltd., including Angelica dahurica, raw rhubarb, astragalus, salvia miltiorrhiza, comfrey, pearl powder, calamine, ...

Embodiment 2

[0119] Example 2 Effects of the components of Shengji Huayu formula on SIRT1 and ICAM-1 in the IFN-γ-induced HaCaT / SIRT1-knockdown HaCaT cell inflammation model

[0120] 1 Materials and methods

[0121] 1.1 Experimental materials

[0122] 1.1.1 Experimental cells

[0123] Cultivation and identification of human epidermal keratinocytes (HaCaT): HaCaT cells come from samples stored in liquid nitrogen for a long time in the laboratory of the research group (originally from the American Type Culture Collection, ATCC). HaCaT cells were routinely resuscitated after removal from liquid nitrogen. A complete medium is prepared, and the ratio of components of the complete medium is as follows: 90% DMEM high-glucose basal medium, 10% fetal bovine serum and 1% penicillin-streptomycin double antibody solution.

[0124] 1.1.2 Drugs and reagents

[0125]

[0126]

[0127] 1.1.3 Instruments and consumables

[0128]

[0129] 1.2 Experimental method

[0130] 1.2.1 Cell culture an...

Embodiment 3

[0158] Example 3 The effect of combination of components of Shengji Huayu formula with pectinolide and SIRT1 overexpression vector on IFN-γ-induced HaCaT cell inflammation model

[0159] Search for SIRT1 on the UCSC genome website (http: / / genome.ucsc.edu / ), clone the expression sequence of SIRT1 (sequence shown in SEQ ID NO:3, primer sequences such as SEQ ID NO:4 and SEQ ID NO:5 shown), cloned into the vector of PCDNA3.1, and verified by sequencing. HaCaT cells were transfected, the protein expression level was verified by WB, and SIRT1 overexpression cells were obtained. Add exogenous recombinant human IFN-γ protein (1ng / ml) and culture medium containing or not containing 1 μM saponin to a 6-well plate, while setting up uninfected cells treated with IFN-γ and 2 μM saponin Viral HaCaT cells served as controls. After 24h, the cell proliferation was detected by MTT method.

[0160] The results in Table 4 show that under the induction of IFN-γ, the proliferation rate of HaCaT ...

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Abstract

The invention relates to application of a glehnia littoralis lactone and SIRT1 up-regulating agent in preparation of a medicine for promoting wound healing of inflammatory diseases. The invention proves that the glehnia littoralis lactone can obviously promote the healing of the skin wound of a diabetic ulcer mouse; the glehnia littoralis lactone can promote proliferation of a HaCaT cell inflammation model, up-regulate expression of SIRT1 and down-regulate expression of ICAM-1, and after the SIRT1 is knocked down, the effects of glehnia littoralis lactone on proliferation promotion and down-regulation of ICAM-1 on the HaCaT cell inflammation model are both inhibited; the multiplication capacity of HaCaT cells overexpressing SIRT1 under the condition of high-level IFN-gamma is obviously improved; the overexpressed SIRT1 and glehnia littoralis lactone synergistically promote the proliferation of a HaCaT cell inflammation model. On the basis, the invention provides application of the glehnia littoralis lactone and SIRT1 up-regulating agent in preparation of a medicine for promoting wound healing of inflammatory diseases or an experimental reagent for promoting proliferation of an IFN-gamma induced HaCaT cell inflammation model.

Description

technical field [0001] The invention relates to the field of medicines, in particular to the application of pectinolide and SIRT1 up-regulators in the preparation of medicines for promoting wound healing of inflammatory diseases. Background technique [0002] Diabetic ulcer is one of the most common complications of diabetes and the leading cause of refractory body surface healing and amputation. According to statistics, various chronic refractory body ulcers account for about 1.7‰ of hospitalized patients in my country, among which diabetes is the primary cause, accounting for as high as 35.3%. The formation mechanism of diabetic ulcer is complex and still not very clear. Once formed, it cannot heal for a long time and is easy to recur. The cost of treatment for this disease is high, which is not only very painful for the patient himself and his family, but also unaffordable financially. According to reports, in the various medical expenses of patients with diabetic ulcer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/37A61K45/06A61P17/02A61P29/00A61P17/00A61P31/04A61P31/12
CPCA61K31/37A61K45/06A61P17/02A61P29/00A61P17/00A61P31/04A61P31/12
Inventor 李福伦邓禹郭冬婕邹家玲郭婉军王怡华亮杨滢瑶陈鸢然
Owner YUEYANG INTEGRATED TRADITIONAL CHINESE & WESTERN MEDICINE HOSPITAL SHANGHAI UNIV OF CHINESE TRADITIONAL MEDICINE
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