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MOF drug carrier for breast cancer treatment and preparation method thereof

A technology of MOF and carrier, which is applied in the field of medicine to achieve the effect of improving multi-drug resistance of cancer, improving the effect of drug-resistant cells, and reducing dosage

Pending Publication Date: 2022-03-01
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research on the design of new nanomaterials combined with the following drug resistance mechanisms (overexpression of p-glycoprotein, overexpression of anti-apoptotic proteins, and high levels of intracellular glutathione) to improve drug resistance in breast cancer

Method used

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  • MOF drug carrier for breast cancer treatment and preparation method thereof
  • MOF drug carrier for breast cancer treatment and preparation method thereof
  • MOF drug carrier for breast cancer treatment and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1: Synthesis and drug-loading performance evaluation of novel metal-organic framework MOF

[0052] Liquid A: Add accurately weighed 0.6g of zinc nitrate (concentration: 0.85μg / mL) to 7mL of N,N-dimethylformamide (DMF), fully dissolve, and then use a 0.8μm filter membrane to filter the dissolved zinc nitrate The solution is filtered to remove insoluble impurities in the raw material.

[0053] Solution B: add accurately weighed 0.5g 2,2'-dithiobipyridine (concentration: 0.7μg / mL) to 7mL dimethyl sulfoxide (DMSO), then add 0.5g 4-dimethylaminopyridine Dissolve, and then filter the solution with a 0.8 μm filter membrane to remove insoluble impurities in the raw material. Add 80mg of polyvinylpyrrolidone accurately weighed to the lysate after passing the film and fully stir;

[0054] Optimization of different raw material ratios:

[0055] Solution 1: Quickly add 7mL of liquid B to 3.5mL of liquid A (the mixing volume ratio of liquid A and liquid B is 1:2), stir an...

Embodiment 2

[0060] The preparation method of Dox@MOF nanoparticles is as follows:

[0061] Mix 400 μL of the MOF nanoparticle dispersion (4 mg / mL) prepared in Scheme 2 of Example 1 with an appropriate amount of Dox solution (2 mg / mL), and stir at 25 ° C for 24 h in the dark to obtain a final MOF concentration of 200 μg / mL , The mixed system with Dox final concentration of 1, 3, 5 μg / mL, that is, Dox@MOF nanoparticles.

Embodiment 3

[0063] The preparation method of Dox / Antisense@MOF nanoparticles is as follows:

[0064] Add 160 μL of Antisense solution (10 mM) to the Dox@MOF nanoparticle dispersion prepared in Example 2, vortex and mix well, and let it stand at room temperature for 2 hours to allow Antisense to adsorb. The final concentration of MOF is 200 μg / mL, and the final concentration of Antisense is 200nM, Dox final concentration of 1, 3, 5μg / mL mixed system, fully reacted to obtain Dox / Antisense@MOF nanoparticles.

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Abstract

The invention relates to the technical field of medicines, in particular to an MOF (Metal Organic Framework) medicine carrier, a preparation method thereof and a medicine carrying system containing the MOF medicine carrier. Zinc nitrate and 2, 2 '-dithiobipyridine are used as main raw materials to synthesize the novel reduction response type metal organic framework, and the novel reduction response type metal organic framework has appropriate particle size (smaller than 200nm) and larger specific surface area, can be used as a drug carrier, loads adriamycin and antisense nucleic acid and wraps hyaluronic acid. In a cytotoxicity test, compared with the IC50 of single Dox to MCF-7 / ADM cells, the IC50 of the Dox loaded in the nano system (Dox / Antisense / HA (at) MOF) to the MCF-7 / ADM cells is greatly reduced, which proves that the nano system has an anti-drug resistance effect, and the dosage of chemotherapeutic drugs is reduced; the anti-drug resistance effect of the novel nano system is determined by measuring the change of the content of glutathione in cells, the expression condition of related proteins and the generation capacity of active oxygen.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a MOF drug carrier for treating breast cancer and a preparation method thereof. Background technique [0002] Cancer morbidity and mortality are currently high in China. Breast cancer is the most common cancer among women in China, as in most other countries. Chemotherapy is one of the most important means of treating malignant tumors. However, for almost all current drugs, as patients use time, cancer cells will develop drug resistance, which will weaken the effect of drugs on cancer cells. The drug resistance of cancer cells is one of the main reasons for the failure of cancer treatment, which may lead to the rapid recurrence of cancer and eventually the death of patients. [0003] With the continuous development of medicine and molecular biology, the mechanisms of tumor drug resistance have been revealed, such as the overexpression of ATP-binding cassette transporter (p-gl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K41/00A61K31/704A61K47/54A61P35/00
CPCA61K47/6949A61K41/0071A61K31/704A61K47/549A61P35/00A61K2300/00
Inventor 杨兆琪龚森怡尚孟迪金坚
Owner JIANGNAN UNIV