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Rabdosia japonica polysaccharide as well as preparation method and application thereof

A technology of polysaccharides and polysaccharides of S. japonica, which is applied in the field of biomedicine and can solve the problems of polysaccharide structure and anti-inflammation of S. basilisk.

Pending Publication Date: 2022-03-01
SHANGAI PHARMA GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the research of the present inventors, the polysaccharide content in S. japonica is 6.3% of the dry weight, but so far there is no report on the structure and anti-inflammatory research of the polysaccharides of S. sativa

Method used

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  • Rabdosia japonica polysaccharide as well as preparation method and application thereof
  • Rabdosia japonica polysaccharide as well as preparation method and application thereof
  • Rabdosia japonica polysaccharide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment Embodiment 1

[0073] Experimental example 1: Effects of crude polysaccharide (XPS), water fraction (XPSW) and homogeneous polysaccharide (XPSW-1) of Saccharomyces japonica on cell viability and NO secretion

[0074] Ana-1 cells in 2 × 10 6 Cells / ml, 100 μL / well were inoculated in 96-well cell culture plates, and the cells were cultured for 24 hours before the experiment began. The cells were divided into the following groups: normal cell control group (Control), test drug alone treatment group, endotoxin (Lipopolysaccharide, LPS) 0.5 μg / ml stimulation group, LPS (0.5 μg / mL) stimulation combined with test drug (different concentration ) treatment group, the positive drug was dexamethasone (DEX). The cells were treated with LPS and drugs for 24 hours. After culturing for 24 hours, collect 200 μL of the cell culture supernatant for subsequent experiments, then add 180 μL of 10% FBS-1640 and 20 μL of 5 mg / mL MTT to the cell wells, and after culturing for 4 hours, discard the supernatant and a...

experiment Embodiment 2

[0079] Experimental Example 2: The regulation of different concentrations of the homogeneous polysaccharide (XPSW-1) of Saccharomyces chinensis (XPSW-1) on the secretion of TNF-α and IL-6 by Ana-1 cells

[0080] Ana-1 cells in 2 × 10 6cells / mL, 100 μL / well inoculated in a 96-well plate and cultured for 24 hours for the experiment. Cells were divided into 4 groups, namely normal cell control group (Control), LPS stimulation group (0.5 μg / mL), LPS (0.5 μg / mL) stimulation combined with XPSW-1 (25, 50, 100 μg / mL) treatment group and positive drug (DEX, 20 μM) treatment group. After co-cultured at 37°C for 24 hours, the supernatant was collected for the determination of cytokines, and the levels of TNF-α and IL-6 in the supernatant were determined according to the experimental operation manual in the ELISA kit.

[0081] The experimental results showed that, compared with the LPS stimulation group alone, LPS stimulation combined with administration of different concentrations of t...

experiment Embodiment 3

[0084] Experimental example 3: Detection of the effect of the homogeneous polysaccharide (XPSW-1) of Saccharomyces japonica (XPSW-1) on the TLR4 pathway in macrophages by Western blotting

[0085] Experimental principle: Toll-like receptors (TLRs) play an important role in the immune system by recognizing specific components expressed by microbial pathogens and initiating immune responses. Among all TLRs, TLR4 specifically senses LPS, transmits signals to host cells such as macrophages, triggers signal transduction, and upregulates the expression of inflammatory cytokines, thereby causing inflammation. TLR4 activates TNF-receptor-associated factor 6 (TRAF6) through a myeloid differentiation factor 88 (MyD88)-dependent pathway. TRAF6 is involved in regulating the nuclear import level of important transcription factor NF-κB. NF-κB participates in regulating the expression levels of various inflammation-related cytokines and mediators in the inflammatory pathway. After phosphor...

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Abstract

The invention relates to rabdosia japonica polysaccharide as well as a preparation method and application thereof. The structure of the rabdosia japonica polysaccharide is shown in the specification, and the rabdosia japonica polysaccharide is mainly composed of arabinose, rhamnose, xylose, glucose and galactose. Experiments prove that the rabdosia japonica polysaccharide has the effects of treating inflammation-related diseases, especially chronic atrophic gastritis and acute lung injury by inhibiting an Ana-1 cell LPS induced TLR4 signal channel, so that the rabdosia japonica polysaccharide has the potential of being developed into the medicine for treating the inflammation-related diseases.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular, the invention relates to a polysaccharide of japonica japonica, its preparation method and its pharmaceutical use. Background technique [0002] Rabdosia japonica (Burm.f.) Hara is the original variety of Rabdosia japonica (Burm.f. Hara), which is a plant of the genus Isodon in the family Labiatae. Weifuchun Tablets is a pure traditional Chinese medicine preparation developed by Hu Qingyutang, a subsidiary of Shanghai Pharmaceutical Group. One of the main sources of medicinal materials of Weifuchun Zhongxiang tea. Weifuchun Tablet has the effects of invigorating the spleen and replenishing qi, promoting blood circulation and detoxification. It is mainly used for the treatment of early gastric cancer, chronic atrophic gastritis, intestinal glandular metaplasia, and intestinal epithelial atypical hyperplasia. adjuvant therapy. Weifuchun Tablet is the only national protected traditio...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P29/00A61P11/00A61P1/04
CPCC08B37/0003A61K31/715A61P29/00A61P11/00A61P1/04
Inventor 王辉俊房庆伟叶冠
Owner SHANGAI PHARMA GRP CO LTD
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