Method for extracting isotope labeled extracellular polymeric substance

An extracellular polymer and isotope labeling technology, which is applied in the field of high-efficiency isotope-labeled chlorella soluble extracellular polymer extraction, can solve problems such as difficulty in obtaining a preset concentration of extracellular polymer, and achieve efficient and rapid labeling, applicable Wide-ranging, fast-concentrating effect

Pending Publication Date: 2022-03-01
NANKAI UNIV
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Problems solved by technology

[0003] The purpose of the present invention is to overcome the deficiencies in the prior art, to provide a method for isotope labeling, separation, purification, concentration, and preservation of extracellular polymers, aiming to solve the problem that there are many methods for extracting extracellular polymers from algae, but for Efficient extraction of isotope-labeled extracellular polymers, difficulty in obtaining preset concentration of extracellular polymers and other technical problems

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  • Method for extracting isotope labeled extracellular polymeric substance
  • Method for extracting isotope labeled extracellular polymeric substance
  • Method for extracting isotope labeled extracellular polymeric substance

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Embodiment 1

[0034] Chlorella culture: Chlorella algae species (FACHB-8) are added to the sterilized medium containing isotopes and cultivated in a light incubator with a culture temperature of 25°C and a light intensity of 30 μmolm -2 the s -1 , Illumination: the dark time ratio is 12h:12h, and the algae growth standard curve prepared by the ultraviolet spectrophotometer at 689nm absorbance value is used to calculate the growth amount of algae.

[0035]

[0036] Extraction of isotope-labeled extracellular polymers by centrifugation: centrifuge the collected algae cells at 4°C and 4200rpm for 15 minutes, remove the supernatant and pass through a 0.45 micron filter membrane, and the filtrate is crude isotope-labeled extracellular polymers.

[0037] Purification and concentration of extracellular polymers: using tangential flow ultrafiltration devices such as figure 2 As shown, the crudely extracted extracellular polymer is quantitatively placed in a sample bottle, and it is concentrate...

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Abstract

The invention belongs to the technical field of isotopic tracing, and relates to the field of basic research of algae, in particular to a method for efficiently extracting an isotopic labeled chlorella soluble extracellular polymeric substance. Comprising the following steps: preparing a culture medium; culturing chlorella to obtain biomass; extracting an S-EPS concentrated solution and a B-EPS concentrated solution; enriching and concentrating the chlorella isotope labeled extracellular polymeric substance; performing dialysis; and cooling and drying to obtain chlorella isotope labeled extracellular polymeric powder. According to the method, efficient and rapid labeling of the chlorella extracellular polymeric substance is realized, the use of isotopes is saved, and the cost is saved. The rapid concentration of the efficiently labeled soluble extracellular polymeric substance is realized, the workload of subsequent dialysis is reduced, and the extraction efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of isotope tracer, relates to the field of basic research of phycology, in particular to a method for extracting soluble extracellular polymer of isotope-labeled chlorella. Background technique [0002] Isotope tracer technology is a technology that uses radioactive isotopes or enriched rare stable nuclides as tracers to study various scientific issues. The extracellular polymer (EPS) is some polymers secreted in vitro by microorganisms, mainly bacteria and algae, under certain environmental conditions. The main components are similar to the intracellular components of microorganisms, which are some high molecular substances, such as polysaccharides, proteins and nucleic acid polymers. The relatively low surface charge and high hydrophobicity of EPS molecules can promote microbial aggregation, protect algae or bacteria from external interference, and play an important role in the field of environmental toxi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/00C12N1/12C12R1/89
CPCC12P1/00C12N1/12
Inventor 姚明奇胡献刚曾辉
Owner NANKAI UNIV
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