Triterpenoids as well as preparation method and application thereof
A kind of technology of triterpenoids and compounds, applied in the field of triterpenoids and preparations thereof
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Embodiment 1
[0110] Take 18.0kg of dry whole herb of A. chinensis, grind it, heat and reflux with 75% ethanol to extract 3 times, the ratio of solid to liquid is 1:8, each time for 2h, recover the solvent under reduced pressure, disperse the extract with distilled water, and use etc. Volume of petroleum ether, chloroform, ethyl acetate and n-butanol extraction 3 times to obtain petroleum ether layer extract 126.4g (extract 1), chloroform layer extract 26.7g (extract 2), ethyl acetate layer extract 36.1g g (extract 3), n-butanol layer extract 162.3g (extract 4).
[0111] The obtained chloroform layer extract 26.7g, adopt normal phase silica gel column chromatography to separate, and mobile phase selects sherwood oil: ethyl acetate (100:0-1:1) and dichloromethane: methanol (50:1-1:1) ) gradient elution, the obtained components were analyzed by silica gel thin-layer chromatography, and 15 parts of eluates were obtained after merging the same components.
Embodiment 2
[0114] Take 27.1 kg of dried Leptopus clarkei (Hook.f.) Pojark., crush it, heat and reflux with dichloromethane to extract 3 times, the ratio of solid to liquid is 1:8, each time for 1.5h, and recover under reduced pressure Solvent, the extract is dissolved in chloroform, acetone and methanol respectively. Take 37.3 g of chloroform layer extract, separate by silica gel column chromatography, elute with a solvent system composed of petroleum ether-ethyl acetate and petroleum ether-acetone, supplemented with 1-2 drops of glacial acetic acid, and obtain 12 parts of fractions, Petroleum ether: ethyl acetate = 50:1 component 1.7g was repeatedly separated by silica gel column chromatography, and the yellow solid powder was separated by preparative HPLC to obtain compounds 3, 4, 13, and 14; at 8:1, for The yellow solid powder was separated by semi-preparative HPLC to obtain compounds 5, 9, 16, 18, and 24. Petroleum ether:ethyl acetate=25:1 component 2.3g was separated. Silica gel c...
Embodiment 3
[0116]Leptopus chinensis (Bunge) Pojark dried whole herb 19.2kg, pulverized, heated and refluxed with 60% ethanol to extract 3 times, the ratio of solid to liquid is 1:8, each time for 1.5h, the solvent is recovered under reduced pressure, used for extract Disperse with distilled water and centrifuge to take the supernatant. After the supernatant is treated with non-polar macroporous resin D101, it is sequentially eluted with water and alcohol gradients, and the eluted fractions of 30% to 100% alcohol are collected. Or HPLC (210nm) screening, screening is rich in the effective part of triterpenoid compound, promptly obtains triterpene crude extract; Gained triterpene crude extract 35.7g, adopts normal phase silica gel column chromatography to separate, with n-hexane-di A solvent system consisting of methyl chloride, dichloromethane-ethyl acetate, and dichloromethane-methanol was used for gradient elution, and the obtained components were analyzed by silica gel thin-layer chroma...
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