Method for screening high-yield strain of biological dust suppressant

A technology for high-yield strains and dust suppressants, applied in biochemical equipment and methods, measurement/inspection of microorganisms, separation of microorganisms, etc., can solve the problem that strains have a greater impact on the yield of biological dust suppressants, and it is difficult for strains to efficiently produce dust suppressants , Low output of biological dust suppressant, etc., to achieve the effect of protecting its own performance, avoiding mutation and bacterial contamination, and ensuring excellent performance

Pending Publication Date: 2022-03-04
CHINA UNIV OF MINING & TECH
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Problems solved by technology

However, the yield of biological dust suppressants synthesized by microbial fermentation is greatly affected by the activity of the strain itself, and it is difficult for ordinary strains to efficiently produce dust suppressants, which leads to low yields of biological dust suppressants and increased production costs

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  • Method for screening high-yield strain of biological dust suppressant

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[0020] Implementation case: Screening high-yielding strains of biological dust suppressants

[0021] First, a medium for activating and expanding the cultured strain is prepared. Preparation of liquid activation medium: Weigh 3.00 g of beef extract, 10.00 g of peptone, and 10.00 g of sodium chloride, add sterile water to make up to 1 L, adjust pH to 7.00, and divide into 2100 ml conical flasks, each containing 100 ml of liquid, Sterilize at 121°C for 15min and cool to room temperature; prepare solid-state activation medium: weigh 3.00g of beef extract, 10.00g of peptone, 10.00g of sodium chloride, and 15g of agar, add sterile water to make up to 1L, and adjust pH To 7.00, sterilize at 121°C for 15min, then cool to 50°C, take out 15ml of the medium and inject it into a blank petri dish, and solidify into a solid after cooling for 10min; prepare growth medium: yeast powder 5.00g, pancreas Peptone 10.00g, sodium chloride 10.00g, add sterile water to make the volume to 1L, adjust...

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Abstract

The invention discloses a method for screening a high-yield strain of a biological dust suppressant, which utilizes the hemolysis characteristic in the process of producing the dust suppressant by the strain and the capability of synthesizing the dust suppressant by the strain for screening, and mainly comprises two parts of screening the high-yield strain and verifying the screened strain. The method comprises the following steps: fully diluting original strains subjected to enlarged culture, inoculating the original strains to a blood agar plate for culture, quantitatively screening the strains according to the sizes of hemolytic rings formed on the blood agar plate by the strains, carrying out fermentation culture on the strains with larger hemolytic rings, extracting the biological dust suppressant subjected to fermentation culture by an acidification precipitation method, and purifying the extracted biological dust suppressant to obtain the biological dust suppressant. And measuring the final dust suppressant yield synthesized by different strains to determine the optimal high-yield strain. Screening is carried out according to the characteristics of the strains, green and environment-friendly culture is selected, the final yield serves as the evaluation standard, the screening method has the advantages of being simple, green and environment-friendly in process and high in screening efficiency, and a new method is provided for screening of high-yield strains in the biological dust suppressant preparation process.

Description

technical field [0001] The invention belongs to the field of dust suppression material synthesis, in particular to a method for screening high-producing strains of biological dust suppressants. Background technique [0002] Dust prevention and control, as an important link in the process of industrial safety production, is an important measure to prevent dust explosion accidents, reduce the incidence of pneumoconiosis, and reduce the pollution of the environment by dust escape. It is of great significance for maintaining safe production, protecting the physical and mental health of employees and reducing environmental pollution. . Wet dust removal is the most important and commonly used technical means in dust prevention and control, that is, water or aqueous solution is used as a medium for wetting, bonding or condensing dust. Dust suppressants can improve the efficiency of wet dust removal by changing the interface properties of the solution. Biological dust suppressants ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C12N1/02C12Q1/06
CPCC12N1/20C12N1/14C12N1/02C12Q1/06
Inventor 王和堂章琦赵侠程思思范岚
Owner CHINA UNIV OF MINING & TECH
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