Composition for preventing or treating neurological or psychiatric disorders comprising extracellular vesicles derived from lactobacillus paracasei

A technology for mental disorders and neurological disorders, which is applied in the direction of medical preparations containing active ingredients, drug combinations, nervous system diseases, etc., and can solve problems such as prevention or treatment of neurological disorders or mental disorders.

Pending Publication Date: 2022-03-04
MD HEALTHCARE INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] However, there is no case where the vesicles secreted by Lactobacillus paracasei are used for the prevention or treatment of neurological disorders or mental disorders

Method used

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  • Composition for preventing or treating neurological or psychiatric disorders comprising extracellular vesicles derived from lactobacillus paracasei
  • Composition for preventing or treating neurological or psychiatric disorders comprising extracellular vesicles derived from lactobacillus paracasei
  • Composition for preventing or treating neurological or psychiatric disorders comprising extracellular vesicles derived from lactobacillus paracasei

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] Example 1. Isolation of vesicles derived from Lactobacillus paracasei

[0103] In order to isolate extracellular vesicles (EV) derived from Lactobacillus paracasei, Lactobacillus paracasei was inoculated into deMan-Rogosa and Sharpe (MRS) medium and cultured at 37 °C and 200 rpm until the absorbance (OD 600nm ) was 1.0 to 1.5, then Lactobacillus paracasei was reinoculated in the Luria Bertani (LB) medium and cultivated. Then, by recovering the culture solution containing the bacterial cells and centrifuging at 4° C. and 10,000 g for 20 minutes, a bacterial cell-depleted supernatant was obtained. The obtained supernatant was filtered again using a 0.22 μm filter and concentrated to a volume of 50 mL or less using a 100 kDa Pellicon 2 Cassette filter membrane (Merck Millipore) and a MasterFlex pump system (Cole-Parmer). Vesicles derived from Lactobacillus paracasei (MDH-001 ) were isolated by re-filtering the concentrated supernatant using a 0.22 μm filter. In the fol...

Embodiment 2

[0104] Example 2. Evaluation of the pharmacokinetic profile of vesicles derived from Lactobacillus paracasei bacteria

[0105] In order to study the pharmacokinetic properties of vesicles derived from Lactobacillus paracasei during oral administration, mice were orally administered vesicles stained with fluorescent staining reagents, and measured from immediately before administration to after administration72 Fluorescence expressed in vivo and in each organ for hours.

[0106] like figure 1 As shown in A and B of Figure 2, it was confirmed that the fluorescently stained L. paracasei-derived vesicles gradually diffused in vivo over time. When each organ was observed separately, fluorescent signals of L. paracasei-derived vesicles were observed in the stomach from 1 hour after oral administration, and fluorescent signals were observed in the small intestine, large intestine, and lung from 3 hours onwards. Furthermore, it was confirmed that the fluorescence signals of the st...

Embodiment 3

[0111] Example 3. Evaluation of the effect of vesicles derived from Lactobacillus paracasei on cognitive function in a mouse model of neurological disorders effect

[0112] The Tg-APP / PS1 mouse is a representative mouse model of degenerative encephalopathy that exhibits the deposition of histologically detectable plaques from 6.5 months and in which cognitive function is stably detected at 7 to 8 months of age Animal models of disorders. After the mice were divided into normal mouse group (WT-CON), degenerative encephalopathy mouse model group (Tg-CON) and in which the dose of 50 μg / mouse was orally administered to the degenerative encephalopathy mouse model After grouping of Lactobacillus vesicles (Tg-Lp EV, MDH-001), this mouse model was used for behavioral and histological testing, such as image 3 shown.

[0113]To assess cognitive function by administering vesicles derived from Lactobacillus paracasei in a mouse model of degenerative encephalopathy, by exposing each...

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Abstract

The present invention relates to a composition for preventing, ameliorating or treating neurological or psychiatric disorders, comprising vesicles derived from Lactobacillus paracasei as an active ingredient. The present inventors confirm that abnormality of cranial nerve function is effectively inhibited when a vesicle derived from Lactobacillus paracasei is administered to a transgenic animal model of neurological disorders, and also confirm that abnormality of cranial nerve function is effectively inhibited when a vesicle derived from Lactobacillus paracasei is administered to an animal model of mental disorders due to mental stress. According to the present invention, mental dysfunction caused by stress and accompanying behavioral disorders are effectively suppressed, and therefore, the Lactobacillus paracasei-derived vesicles according to the present invention can be effectively used in the development of drugs or health-care functional foods for preventing neurological disorders or mental disorders, improving symptoms thereof, treating neurological disorders or mental disorders, and the like.

Description

technical field [0001] The present invention relates to a composition for preventing, improving or treating neurological disorders or mental disorders, comprising vesicles derived from Lactobacillus paracasei as an active ingredient and the like. [0002] This application claims priority and benefit to Korean Patent Applications 10-2020-0072685 and 10-2020-0169167 filed with the Korean Intellectual Property Office on June 16, 2020 and December 7, 2020, respectively, and the descriptions of these applications All the contents disclosed in the drawings and drawings are incorporated into this application. Background technique [0003] Since the 21st century, acute infectious diseases, which were considered to be epidemics in the past, have become less important, while chronic diseases accompanied by immune dysfunction caused by the incompatibility between human and microbiome have changed the disease pattern and become the key factors that determine the quality of life and huma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A99Z99/00
CPCA61P25/00A61K35/747A23L33/135A23V2002/00A23V2200/316A23V2200/322A23V2200/3262A23V2200/328A23V2200/332A61K35/744A61P3/00A61P21/00A61P25/18
Inventor 金润根
Owner MD HEALTHCARE INC
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