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Leukocyte hemolytic agent, preparation method and hematology analyzer

A hemolytic agent and leukocyte technology, applied in the field of leukocyte analysis, can solve the problems of non-classification or wrong classification, increase, affecting the results of leukocyte count and classification accuracy, etc. Effect

Pending Publication Date: 2022-03-08
SHENZHEN GOLDSITE DIAGNOSTICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Lipidemia means that the patient eats a large amount of high-fat food, which can cause a temporary increase in blood fat. When detecting hyperlipidemia samples, the blood cell analyzer is prone to non-classification or misclassification, which affects the white blood cell count results and classification accuracy.

Method used

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  • Leukocyte hemolytic agent, preparation method and hematology analyzer
  • Leukocyte hemolytic agent, preparation method and hematology analyzer
  • Leukocyte hemolytic agent, preparation method and hematology analyzer

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preparation example Construction

[0056] The present invention also proposes a preparation method of a leukocyte hemolysis agent, which is used to prepare the above leukocyte hemolysis agent, comprising the following steps:

[0057] Mixing the quaternary ammonium salt cationic surfactant, nonionic surfactant, buffer and protective agent with the solvent, stirring and dissolving to obtain a preliminary solution;

[0058] The preliminary solution is filtered to obtain the leukocyte hemolyser.

[0059] Mix and stir the quaternary ammonium salt cationic surfactant, nonionic surfactant, buffer and protective agent with the solvent until dissolved, then filter the preliminary solution to filter the impurity particles in the preliminary solution and reduce the impact of impurities on the test results. The filter membrane may be 0.2 μm.

[0060] In one embodiment, the step of obtaining the preliminary solution includes: weighing a quaternary ammonium salt cationic surfactant, a nonionic surfactant, a buffer and a pro...

Embodiment 1

[0066] Weigh 2.0g of dodecyltrimethylammonium chloride, 3.0g of cetyltrimethylammonium bromide, 7.0g of lauryl polyoxyethylene ether (AEO-9), 5.0g of sodium chloride, Mix 3.0 g of methanol and 0.05 g of dimethyl yellow, add deionized water to make up to 1 L, stir at room temperature until completely dissolved, and filter through a 0.2 μm filter membrane to obtain a leukocyte hemolysis agent.

Embodiment 2

[0068] Weigh 7.0g of dedecyltrimethylammonium bromide, 2.0g of hexadecyltrimethylammonium bromide, 2.0g of Trion X-100, 8.0g of sodium chloride, 1.0g of isopropanol, and 0.05g of methyl orange Mix, add deionized water to make up to 1L, stir at room temperature until completely dissolved, filter through a 0.2 μm filter membrane, and filter to obtain leukocyte hemolysis agent.

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Abstract

The invention discloses a leukocyte hemolytic agent, a preparation method and a hematology analyzer. The leukocyte hemolytic agent comprises a quaternary ammonium salt cationic surface active agent, a nonionic surface active agent, a buffering agent, a protective agent and dye. Wherein the nonionic surfactant is prepared from at least one of dodecyl polyoxyethylene ether, polyethylene glycol octyl phenyl ether, polyoxyethylene nonyl phenyl ether, Tween 80, Tween 20, polyoxyethylene lauryl ether, polyether L-35 and polyether F-6. According to the invention, the accuracy of counting and classified detection of leukocytes in a lipid sample is ensured.

Description

technical field [0001] The invention relates to the technical field of white blood cell analysis, in particular to a white blood cell hemolysis agent, a preparation method and a blood cell analyzer. Background technique [0002] Blood consists of plasma (55%) and blood cells (45%). Blood cells are called "blood cells" in medicine, dissolve in human blood throughout the body, and mainly include red blood cells (RBC), white blood cells (WBC) and platelets (PLT). Red blood cells are mainly responsible for delivering oxygen to the body, while white blood cells play an important role in the human immune system and protect people's health. White blood cells are extremely important cells in the blood of the human body. As an important component of the immune system, they help the body resist pathogens and foreign substances, so white blood cells are often called immune cells. There are five types of white blood cells: lymphocytes, monocytes, neutrophils, eosinophils, and basophil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/10
CPCG01N15/10G01N2015/1006G01N2015/016G01N2015/1024
Inventor 伍耀婵陈明峰郑筱雯
Owner SHENZHEN GOLDSITE DIAGNOSTICS