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Lactobacillus plantarum Q16 with function of relieving non-alcoholic fatty liver caused by high fat diet

A Lactobacillus plantarum, non-alcoholic technology, applied in the field of Lactobacillus plantarum Q16, can solve the problems of polyphenols absorption and processing technology restricting development, to improve the intestinal micro-ecosystem, inhibit abnormal accumulation, and improve energy metabolism Effect

Pending Publication Date: 2022-03-11
NANJING AGRICULTURAL UNIVERSITY
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the taste of dietary fiber and oligosaccharides, the absorption and processing technology of polyphenols restrict their development

Method used

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  • Lactobacillus plantarum Q16 with function of relieving non-alcoholic fatty liver caused by high fat diet
  • Lactobacillus plantarum Q16 with function of relieving non-alcoholic fatty liver caused by high fat diet
  • Lactobacillus plantarum Q16 with function of relieving non-alcoholic fatty liver caused by high fat diet

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Experimental program
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Embodiment Construction

[0031] Through specific embodiment, and in conjunction with accompanying drawing, the present invention is further elaborated:

[0032] 1. Identification and cultivation of Lactobacillus plantarum Q16

[0033] The yogurt made by herdsmen in Qinghai was transported to the laboratory at low temperature. Under aseptic conditions, the yogurt sample was mixed with sterile MRS at a volume ratio of 1:100 to enrich lactic acid bacteria. Spread the cultivated flora evenly on the MRS solid medium, pick a single colony and culture again, streak culture the cultured bacteria, pick a single colony again, continue to expand the culture, repeat the above steps 3 times , Pick a single colony and carry out sequencing identification. A single colony was identified as Lactobacillus plantarum, which we named as Lactobacillus plantarum Q16 (attached figure 1 ).

[0034] 2. Production of Lactobacillus plantarum Q16 bacterial suspension

[0035] Centrifuge the cultured Lactobacillus plantarum Q1...

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Abstract

The invention provides a lactobacillus plantarum Q16 capable of relieving non-alcoholic fatty liver function caused by high fat diet, the classification name of the lactobacillus plantarum is lactobacillus plantarum, the lactobacillus plantarum is preserved in China Center for Type Culture Collection on October 22, 2021, the address is Wuhan University, Wuhan, China, the postcode is 430072, and the preservation number is CCTCC NO: M20211311. The strain can effectively reduce the body weight, liver weight and fat weight of a high-fat diet mouse, improve lipid metabolism in the liver, inhibit accumulation of triglyceride and improve energy metabolism in the liver; in addition, the strain also has the capability of optimizing the intestinal flora structure, and has a good probiotic function.

Description

1. Technical field [0001] The invention relates to a strain of Lactobacillus plantarum Q16 capable of relieving non-alcoholic fatty liver caused by high-fat diet, which belongs to the field of food microorganisms. 2. Background technology [0002] In the past few decades, due to people's unreasonable dietary structure and lifestyle changes, the number of patients with non-alcoholic fatty liver has increased sharply, and it has become a serious public health problem worldwide. In addition, the occurrence of non-alcoholic fatty liver can induce other diseases, such as liver cirrhosis, type 2 diabetes, hypertension, cardiovascular disease, etc. Therefore, non-alcoholic fatty liver seriously endangers human health. A large number of studies have shown that the occurrence of non-alcoholic fatty liver is related to the disorder of the intestinal flora structure in the body, which is specifically manifested in the decrease of the diversity of the flora and the proportion of commens...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P1/16A61P1/00A61P3/04A61P3/06A23L33/135C12R1/25
CPCA61K35/747A61P1/16A61P1/00A61P3/04A61P3/06A23L33/135A23V2002/00A23V2400/169A23V2200/32A23V2200/332A23V2200/3262A23V2200/30
Inventor 陆兆新唐超李金良吕凤霞别小妹赵海珍
Owner NANJING AGRICULTURAL UNIVERSITY
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