Preparation method of blood peptide chelate

A chelate and blood peptide technology, which is applied in the field of blood peptide chelate preparation, can solve the problems of limiting the application of blood peptide and its chelate, uncontrollable molecular weight, and low chelation rate, so as to improve the utilization value, The preparation method is simple, and the effect of improving the chelation rate

Active Publication Date: 2022-03-15
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on animal blood peptides mainly focuses on the extraction and preparation of active ingredients such as SOD, antioxidant peptides, and blood pressure-lowering peptides, while there are relatively few studies on active peptide chelates, and the traditional preparation methods have low chelation rates and low cost. High and uncontrollable molecular weight greatly limit the application of blood peptide and its chelate in food, pet food, functional food or medicine

Method used

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  • Preparation method of blood peptide chelate
  • Preparation method of blood peptide chelate
  • Preparation method of blood peptide chelate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: 100 kg of fresh pig blood was anticoagulated and impurity removed, then centrifuged at 3000 g for 15 min to collect precipitated blood cells. Add water and stir evenly to adjust the blood cell concentration to about 20% to swell the cells, and at the same time carry out homogenization treatment for 40 minutes; centrifuge at 3000g for 10 minutes, and collect the supernatant hemoglobin. Add 4‰ alkaline protease, 3‰ neutral protease, and 3‰ compound flavor protease to the hemoglobin solution for enzymolysis. The enzymolysis time is 5 hours. After the enzymolysis, the temperature is raised to 95°C and kept for 15 minutes to inactivate the enzyme, and the enzymolysis is obtained. Centrifuge the enzymolysis solution with a 4000g tube centrifuge for 10 minutes, then filter and purify the supernatant with a 20nm nanofiltration membrane, and ultrasonically treat the nanofiltration solution for 5 minutes. The temperature is 55°C, the frequency is 20kHz, and the power i...

Embodiment 2

[0027] Example 2: Take 100 kg of fresh anticoagulated chicken blood, centrifuge at 3000g for 15min, add 5 times the volume of water to the precipitate and stir for 40min, centrifuge at 3000g for 10min to obtain a hemoglobin solution, add 4‰ alkaline protease and 3‰ neutral protease and 3‰ compound flavor protease for enzymolysis, the enzymolysis time is 6 hours, after the enzymolysis, the temperature is raised to 95°C and kept for 15 minutes to inactivate the enzyme, and the enzymolysis solution is obtained, and the enzymolysis solution is centrifuged in a 4000g tubular centrifuge for 10 minutes. The supernatant was filtered and purified with a 20nm nanofiltration membrane, and then sonicated for 15 minutes. The working conditions of the sonication were 10-20s working time, 5-10s resting time, 55°C ultrasonic temperature, 20kHz frequency, and 200W power. Then adjust its pH to 4.5 and add 8% FeSO 4 .7H 2 O, stirring at 40 rpm for 40 min at 37°C to obtain a chelating solution, ...

Embodiment example 3

[0028] Implementation Case 3: The procedure is the same as in Example 2. After extracting hemoglobin from fresh chicken blood, add 4‰ alkaline protease, 3‰ neutral protease and 3‰ compound flavor protease for enzymolysis. The enzymolysis time is 5 hours. Rise to 95°C and keep it for 15 minutes to inactivate the enzyme to obtain the enzymatic hydrolysis solution. Centrifuge the enzymatic hydrolysis solution with a 4000g tube centrifuge for 10 minutes. The supernatant is filtered and purified with a 20nm nanofiltration membrane and then ultrasonicated for 10 minutes. The ultrasonic working condition is working The time is 10-20 s, the rest time is 5-10 s, the ultrasonic temperature is 55°C, the frequency is 20kHz, and the power is 200W. Then adjust its pH to 4 and add 15% ZnSO in the resulting nanofiltrate 4 .7H 2 O, stirring at a speed of 40 rpm for 60 min at 30° C. to obtain a chelating solution, which was concentrated and then spray-dried to obtain the animal blood peptide c...

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Abstract

The invention relates to a preparation method of a hemepeptide chelate, which comprises the following steps: adding an anticoagulant into fresh animal blood, centrifuging, collecting a precipitated hemoglobin solution, carrying out enzymolysis treatment by using bacillus licheniformis alkaline protease, bacillus subtilis neutral protease and aspergillus oryzae compound flavourzyme, and separating and purifying crude peptide; after ultrasonic pretreatment, a chelating ligand is added into the polypeptide solution, and the animal blood chelating peptide is obtained. The preparation method is simple, the steps are easy to operate, the low-molecular-weight hemepeptide is obtained after enzymolysis separation and purification, the chelating rate is increased, and the application range of the hemepeptide product is widened.

Description

technical field [0001] The invention relates to a preparation method of a blood peptide chelate, belonging to the technical field of animal blood deep processing. Background technique [0002] Small peptide chelate is a chelate with a ring structure formed by combining trace or macro elements with active peptides through coordination bonds. It is a new type of trace element additive or macro element enhancer. It can be used as a food additive or nutritional fortifier in the food industry and pet feed. However, due to the presence of a large number of inorganic acid groups, it is easy to form precipitates and affect the absorption of metal elements such as calcium. [0003] Animal blood, especially blood cells, is rich in protein, vitamins, various trace elements and other active ingredients, which can prevent anemia, lower cholesterol, anti-aging and improve immunity. Therefore, the blood polypeptide can be used as an additive or a nutritional enhancer in food or medicine....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/805C12P21/06C07K1/107C07K1/34A23K20/20A23K10/24A23K20/24A23K20/147A23L33/165A23L33/18A23J1/06A23J3/34A23J3/12
CPCC07K14/805C12P21/06A23K20/30A23K10/24A23K20/24A23K20/147A23L33/165A23L33/18A23J1/06A23J3/345A23J3/12A23V2002/00A23V2200/30A23V2250/1578A23V2250/1642A23V2250/1592A23V2250/55
Inventor 杨静邹烨王道营徐为民诸永志石景杨彪马晶晶张新笑闫征卞欢
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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