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Establishment method and application of nibea albiflora swim bladder cell line

A method of establishment, a technology of meager fish, applied in the field of establishment of light-colored meager swim bladder cell line, can solve problems such as the mechanism of collagen synthesis of light-colored meager that has not yet been established

Pending Publication Date: 2022-03-15
SHANTOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are only a few fish (grass carp, goldfish, and grouper) related reports on the application of swim bladder cell lines in pathological research, and there are no light-colored yellow croaker swim bladder cell lines and the use of swim bladder cell lines for collagen synthesis Successful reports of mechanism-related studies

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  • Establishment method and application of nibea albiflora swim bladder cell line
  • Establishment method and application of nibea albiflora swim bladder cell line
  • Establishment method and application of nibea albiflora swim bladder cell line

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Embodiment Construction

[0041] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0042] 1. Construction of light-colored yellow croaker swim bladder cell line

[0043] 1. Preparation of Cell Culture Medium

[0044] Get Gibco's Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F12, 1:1) medium, add fetal bovine serum accounting for 20% of the total volume of the medium, basic fibroblast growth factor (bFGF) of 20ng / ml ), 40ng / ml of insulin-like growth factor (IGF-I), 20ng / ml of epidermal growth factor (EGF), 100IU / ml of penicillin, and 100μg / ml of streptomycin to make complete cell culture medium.

[0045] 2. Primary Cell Culture

[0046] The light-colored croaker (50g) used in the present invention comes from the Nan'ao Marine Biology Experimental Station of Shantou University, and the primary cell culture time is April 2019. It ...

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Abstract

The invention relates to the technical field of biology, in particular to an establishment method of a nibea albiflora swim bladder cell line and application of the nibea albiflora swim bladder cell line in collagen synthesizing.The establishment method comprises the following steps that S1, the nibea albiflora swim bladder cell line is established through primary culture and subculture, and at present, 50 generations of the nibea albiflora swim bladder cell line are subcultured; and S2, incubating the cells by using a TGF-beta reagent, collecting the cells after 48 hours, and exploring the change of collagen related gene expression quantity and collagen content. Establishment of the nibea albiflora swim bladder cell line as an in-vitro model for researching a collagen synthesis mechanism and swim bladder nutrition regulation is very necessary, the blank in related fields is filled, and a new thought is provided for developing a method for improving the quality of swim bladders.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for establishing a swim bladder cell line of light-colored yellow croaker and its application. Background technique [0002] Fish maw (dried fish maw), also known as fish maw, is rich in gelatin and has been a valuable tonic since ancient times, known as "marine ginseng". The light-colored croaker is a carnivorous fish belonging to the genus crocodile in the family Scrotoaceae. Because of its fresh and tender meat and delicious taste, especially its fish glue (white flower glue) has high nutritional and medicinal value, and belongs to one of the four famous glues (the price of high-quality fish glue is 10,000-15,000 yuan / kg). High economic value and breeding prospects. In recent years, with the continuous improvement of national living standards, more and more people have begun to pay attention to health preservation. As an ideal high-protein and low-fat food, isinglass is...

Claims

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Application Information

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IPC IPC(8): C12N5/071C07K14/78A01N1/02
CPCC12N5/0602C07K14/78A01N1/0221C12N2501/105C12N2501/115C12N2501/11C12N2501/15Y02A40/81
Inventor 林帆袁钰莹林佳丽余俊郭浩棘张浩然王树启
Owner SHANTOU UNIV