Enterococcus monteilii and application thereof in fermentation of Chinese herbal medicines

A technology for Enterococcus montelii and fermentation of Chinese herbal medicines, applied in the field of microorganisms, can solve the problems of low output and can not be fully utilized, etc., and achieves the effects of simple operation, broad practical application prospects and strong practicability.

Active Publication Date: 2022-03-18
ZHENGZHOU UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using traditional methods such as pressing, steam distillation, and solvent extraction to directly extract the active ingredients in Astragalus membranaceus, the output is low and cannot be fully utilized

Method used

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  • Enterococcus monteilii and application thereof in fermentation of Chinese herbal medicines
  • Enterococcus monteilii and application thereof in fermentation of Chinese herbal medicines
  • Enterococcus monteilii and application thereof in fermentation of Chinese herbal medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Isolation and culture of strains derived from cow dung:

[0028] (1) Sample collection and processing

[0029] Prepare 12 sterile 10mL glass sample collection tubes, collect Simmental (9 heads), Charolais (2 heads), and Black and White Flowers (1 head), a total of 12 cow feces samples of three breeds. The feed these cattle eat includes whole price concentrate, whole plant silage, hay, corn, wheat bran and Mushengyuan (fermented traditional Chinese medicine). Collect and accurately weigh 0.5 g of feces samples from each of the above 12 cows, add 9.5 mL of sample diluent (0.9 % mass concentration of normal saline), and add numbers 1-1, 1-2, 1-3, 2-1, 2-2, 2-3, 3-1, 3-2, 3-3, 4-1, 4-2, 4-3 in the sample collection tube and shake quickly to mix. were diluted to 10 -5 、10 -6 、10 -7 multiples of three.

[0030] MRS agar medium

[0031] Peptone 10g / L, beef extract powder 5g / L, yeast extract powder 4g / L, glucose 20g / L, Tween-1ml / L, dipotassium hydrogen phosphate 2g / L, a...

Embodiment 2

[0040] Screening strains that can increase the content of active components in Astragalus membranaceus

[0041] Astragalus decoction pieces are Mongolian Astragalus. Astragalus was naturally dried, crushed to 60 mesh, and stored for later use.

[0042] (1) Recovery and passage of strains

[0043] Prepare MRS medium, and use high pressure for standby. Inoculate 1mL of bacterial liquid into 50mL of prepared MRS liquid medium according to the proportion of 2% of the 12 isolated bacterial strains stored in the freezer, and incubate anaerobically at 37°C for 24h. The resuscitated strains continued to be inoculated into new MRS liquid medium at a ratio of 2%, and thus passed to the fourth generation.

[0044] (2) Preparation and fermentation of fermentation medium

[0045] Prepare 12 groups of fermentation media of 2 L each, and set up 3 replicates. Take 200 g of crude drug Astragalus root as the control group, and compare the content changes of the main components of Astragalus...

Embodiment 3

[0061] The preparation embodiment of the fermented bacterial agent that comprises bacterial strain MY-YD-1

[0062] Strain MY-YD-1 (inoculum size is 10 3 CFU) into the slant of MRS medium for cultivation: time: 16-24 hours, environment: anaerobic fermentation, temperature: 36.5-37.5°C, initial pH: 6.0-6.4.

[0063] The composition of MRS slant medium is: 1% peptone, 1% beef extract, 0.5% yeast powder, 0.2% diamine citrate, 2% glucose, 0.5% sodium acetate, 0.2% dipotassium hydrogen phosphate, 0.0058% MgSO 4 ·7H 2 O, 0.025% MnSO 4 4H 2 O, 0.1% (v / v) Tween-80, pH 6.2, 3% agar.

[0064] The culture obtained in the previous step (the inoculum size was 10 3 CFU) is inserted into primary or secondary seed medium for cultivation: time 24-48 hours, environment: anaerobic fermentation, temperature: 36.5-37.5°C, rotation speed 100r / min, initial pH: 6.0-6.4, and fermentation is obtained after cultivation Bacteria.

[0065] The composition of the seed medium is: 1% peptone, 1% beef...

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Abstract

The invention belongs to the field of microorganisms, and relates to enterococcus monteilii, in particular to enterococcus monteilii and application thereof in fermentation of Chinese herbal medicines. The preservation number of the strain is CCTCC NO: M 20211504, the classification name is Enterococcus mudtii MY-YD-1, the preservation unit is China Center for Type Culture Collection, the preservation time is November 29, 2021, and the preservation address is Wuhan University, Wuhan, China. When the strain is used for fermenting the traditional Chinese herbal medicine astragalus membranaceus, the release amount of the effective components astragalus polysaccharide, flavonoid and saponin compounds in the astragalus membranaceus can be greatly increased. The method for fermenting Chinese herbal medicines by virtue of strains is an effective method for improving active ingredients. The enterococcus monteilii MY-YD-1 strain disclosed by the invention is good in astragalus mongholicus fermentation performance and high in yield of active ingredients, and can be used for small-scale and large-scale industrial production in the future, so that very strong economic benefits are brought.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a strain of Enterococcus monsonii, in particular to a strain of Enterococcus monsonii ( Enterococcus mundtii ) and its application in fermented Chinese herbal medicine. Background technique [0002] Astragalus is the dried root of the leguminous plant Astragalus mongolica or Astragalus membranaceus. It is a traditional Chinese medicine with a long history. Anti-oxidation, anti-aging, anti-radiation damage, cardiovascular protection, immune regulation, liver and kidney protection, blood sugar regulation, blood lipid lowering, blood pressure lowering, diuretic and other effects. Not only that, it has broad application prospects in food, health care products, cosmetics and other industries. The main components in Astragalus are astragalus polysaccharides, flavonoids, saponins, amino acids, etc. In addition, Astragalus also contains alkaloids, lignans, sterols, and trace elements, which...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P17/06C12P19/04C12P33/20C12R1/01
CPCC12N1/20C12P19/04C12P17/06C12P33/20
Inventor 魏勇军阳雨蝶张永
Owner ZHENGZHOU UNIV
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