Naphthalimide fluorescent molecular probe for detecting copper ions as well as preparation method and use method of naphthalimide fluorescent molecular probe

Abstract

The invention discloses a naphthalimide fluorescent molecular probe for copper ion detection and a preparation and use method thereof, and relates to a fluorescent molecular probe and preparation and application thereof. The invention aims to solve the problems that the existing Cu < 2 + > fluorescent probe is easily influenced by pH and interfered by coexisting metal ions, and is poor in water solubility, low in sensitivity and poor in selectivity. The fluorescent molecular probe is N-butyl-1-hydroxy-2-[(2-hydroxy naphthylidene) hydrazine methyl] naphthalimide, and the molecular probe is N-butyl-1-hydroxy-2-[(2-hydroxy naphthylidene) hydrazine methyl] naphthalimide. According to the present invention, N-butyl-4-hydroxy-3-formyl-1, 8-naphthalimide and 2-hydroxy-1-naphthylhydrazone are subjected to condensation so as to obtain the product, and the yield is 70-79%; the fluorescent molecular probe is dissolved in a mixed solution of dimethyl sulfoxide and an HEPES buffer solution, the existence of copper ions is judged according to the absorbance value or fluorescence intensity change of a test sample before and after the copper ions are added, and the fluorescent molecular probe can be used for detecting Cu < 2 + > in food and environment.

Description

technical field [0001] The invention belongs to the technical field of preparation and application of fluorescent molecular probes for copper ion detection, and specifically relates to a naphthalimide fluorescent molecular probe for copper ion detection and methods for preparing and using the same. Background technique [0002] Copper is an essential trace element and is the third most abundant trace element in the human body (after iron and zinc). Abnormal copper metabolism may hinder the synthesis of ceruloplasmin (CP), which is widely deposited in organs and tissues such as liver and brain, leading to liver cirrhosis and hepatolenticular degeneration. Copper also affects iron metabolism and hematopoietic function, thereby affecting the central nervous system, and affects bone and connective tissue metabolism, energy metabolism, cardiovascular system and endocrine system. On the one hand, the lack of Cu 2+ Can seriously affect children's brain development, on the other h...

AI Technical Summary

Problems solved by technology

[0007] The present invention is to solve the existing identification Cu 2+ The fluorescent probe of the present invention is susceptible to the problems of pH influence, interference of coexisting metal ions, poor water solubility, low sensitivity and poor selectivity, and provides a naphthalimide fluorescent molecular probe for copper ion detection and its preparation and use method

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