Rice endophytic streptomyces tauioides Ahn75 and application thereof
A technology of taupe and streptomyces, applied in the field of microorganisms, can solve problems affecting human health, easy loss of resistance, pollution of the environment, etc., achieve good development and application prospects, and inhibit growth
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Embodiment 1
[0031] Screening and purification of embodiment 1Ahn75 bacterial strain
[0032] Collect mildly diseased and non-infected rice tissues from rice fields in Sanya City, Hainan, clean and disinfect the surface, then cut the stems into small pieces about 1 cm long, place them in TWYE medium, and cultivate them at 37°C until endogenous release Bacteria were separated from the tissue, picked out and transferred to 1 / 2 PDA medium and streaked for isolation and purification, so as to obtain a pure culture of the bacterium. The purified strains were suspended in 25% glycerol and stored in a -80°C refrigerator.
[0033] Inoculate fresh bacterial cakes of different physiological races of Pyricularia oryzae in the center of the ISP2 medium with a diameter of 90 mm, and then inoculate actinomycete strains with sterile toothpicks at 120° from the center of the bacterial cakes at 3 cm, and set no bacterial strain treatment as a control , each treatment was repeated 3 times, cultured at 28°C...
Embodiment 2
[0035] Identification of embodiment 2Ahn75 bacterial strain
[0036] 1. Morphological observation of the strain: the strain is cultured on ISP2 medium, the initial colony is round, the air filaments are yellow and white, the early spores are grayish green, the late spores are earthy yellow, the base filaments are light yellow brown, and reddish-brown pigment is produced over time. Take the strain cultured on the ISP2 medium and observe it under the phase contrast microscope. The spore filaments are straight or curvy, the conidia are spherical to oval, and the surface of the spores is smooth. For the early and late colony morphology and hyphae and spore morphology of the Ahn75 strain, see figure 1 .
[0037] 2. Strain identification:
[0038] Genomic DNA of Actinomycetes Ahn75 was extracted using Shanghai Bioengineering Co., Ltd. Bacterial Total DNA Extraction Kit, using it as a template and using 27F / 765R and 704F / 1492R as primers (see Table 1 for primer sequences) to amplif...
Embodiment 3
[0041] The antibacterial rate of embodiment 3 bacterial strains to different rice blast physiological races
[0042]The antibacterial activity of the Ahn75 bacterial strain to various sources of different physiological races of rice blast was detected by the plate confrontation test (the operation process was the same as in Example 1). The results are shown in Table 2. Ahn75 showed high antibacterial activity against 40 different physiological races of rice blast pathogens, and the antibacterial rate against 10 strains of Pyrospora oryzae exceeded 50%.
[0043] Table 2 Inhibitory activities of Ahn75 on the mycelia growth of different physiological races of Oryza spp.
[0044]
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