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Method for identifying porphyra haitanensis in different producing areas and different harvesting periods

A technology of seaweed and harvest period, applied in the field of identifying seaweed in different origins and harvest periods, can solve problems such as difficulty in distinguishing seaweed and consumers, and achieve the effects of accurate results, wide applicability, and improved identification accuracy.

Pending Publication Date: 2022-03-22
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult for consumers to distinguish altar laver from different origins and different harvest periods from the color and shape of laver, and the nutritional content of altar laver in different origins and different harvest periods will change continuously. Therefore, it is necessary to provide an effective detection method for different origins and harvests. The method of Qitan seaweed

Method used

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  • Method for identifying porphyra haitanensis in different producing areas and different harvesting periods
  • Method for identifying porphyra haitanensis in different producing areas and different harvesting periods
  • Method for identifying porphyra haitanensis in different producing areas and different harvesting periods

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Experimental program
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Effect test

Embodiment 1

[0021] The chemical components in the various nutrients of laver will change continuously with the different growth stages. Among them, laver polysaccharide is one of the main components of laver. Accounting for 20-40% of the dry weight of laver, laver polysaccharide belongs to galactan sulfate, mainly composed of galactose, 3,6-endether galactose and sulfate group. The content and proportion of polysaccharide components in algae laver in different production areas and different harvest periods will also change continuously.

[0022] After a lot of research, the basic structure of laver polysaccharides has been determined. Its basic structural unit is a disaccharide composed of 3-β-D-galactoside-1,4-α-L-3,6-endether galactose. In some L-galactose residues, there is a 6-sulfate group, and laver polysaccharides also contain other substituent groups. However, due to differences in laver varieties, growth environments and other conditions, different laver polysaccharides have d...

Embodiment 2

[0044] Based on the above-mentioned embodiments, this embodiment provides a more specific practical application description for each step of the identification method of algae laver in different origins and different harvest periods.

[0045] In this example, 5 batches of algae laver from different origins and different harvest periods were selected and marked as groups A, B, C, D, and E respectively. Groups A, B, and C were in Xiapu, Fujian Province, which were in different harvest periods in mid-November (one water) , Altar laver in mid-December (Eshui) and late January (Sanshui). D and E are Yishuitan laver from Xiangshan and Wenzhou, Zhejiang. After pretreatment, water extraction and alcohol precipitation, enzymatic hydrolysis and Sevage method to remove protein in sequence, polysaccharide samples of Porphyra laver were obtained, and the composition of each polysaccharide sample was determined by protein content, total sugar content, and sulfate content, so as to further d...

Embodiment 3

[0065] refer to figure 2 and image 3 In this example, ion Fourier transform infrared spectroscopy and ion chromatography are specifically described.

[0066] (1) Utilize Fourier Transform Infrared Spectroscopy to analyze Porphyra laver in different harvest periods:

[0067] When a continuous wavelength infrared light source irradiates a sample, the molecules in the sample will absorb or partially absorb certain wavelengths of light, and the unabsorbed light will reach the detector. The light analog signal obtained by the detector through the sample is subjected to analog-to-digital conversion and Fourier transform to obtain a single-beam spectrum with sample information and background information, and then the same detection method is used to obtain the background single-beam spectrum of infrared light without passing through the sample. The background single-beam spectrum is subtracted from the single-beam spectrum of the sample to generate an infrared "fingerprint" spect...

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Abstract

The invention discloses a method for identifying porphyra haitanensis in different producing areas and different harvesting periods. The method comprises the following steps: pretreating a porphyra haitanensis sample to be identified, and extracting porphyra haitanensis polysaccharide; measuring the content of sulfate radicals in each polysaccharide component by using a barium sulfate turbidimetric method, and quickly judging the producing area and the harvesting time of the porphyra haitanensis; monosaccharide composition is analyzed through ion chromatography, and the harvest time of the porphyra haitanensis is accurately judged; carrying out characteristic group analysis by utilizing Fourier transform infrared spectroscopy, and accurately judging the harvest time of the porphyra haitanensis; and analyzing the fine structure by using nuclear magnetic resonance hydrogen spectrum to further accurately verify the producing area and the harvesting time of the porphyra haitanensis. The method has the beneficial effects that the analysis method can be flexibly selected according to identification precision requirements, the applicability is wide, the production place and the specific harvest time of the porphyra haitanensis polysaccharide can be quickly and accurately judged, and the result is accurate.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a method for identifying algae laver from different origins and different harvest periods. Background technique [0002] In recent years, Porphyrahaitanensis belongs to red algae and is one of the main species of seaweed artificially cultivated in my country. Xiangshan and Wenzhou in Zhejiang, and Xiapu in Fujian are the main producing areas of cultivated algae. The laver they produce is rich in nutrients such as protein, polysaccharides and vitamins, and has good quality, which is popular among consumers. Altar laver is harvested in stages, and the general harvest period starts from mid-November and lasts until late January of the next year. The laver harvested for the first time is called toushui or yishui, which has the best taste and the highest market price. The following crops are called Dishui, Sanshui and Moshui in turn. The later the altar laver is harvested, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/3563G01N24/08G01N30/96G01N30/86
CPCG01N21/3563G01N24/087G01N30/96G01N30/8634
Inventor 傅玲琳王彦波王翀叶自强
Owner ZHEJIANG GONGSHANG UNIVERSITY