Novel coronavirus immunotyping detection method

Abstract

The invention belongs to the technical field of peripheral blood immune cell characteristic spectrum research, and particularly relates to a novel coronavirus immune typing detection method which comprises 38 cell membrane surface antibodies, 4 intracellular antibodies, a cell nucleus marker, a cell death and living marker, a cell staining buffer solution, a cell membrane punching agent and a cell nucleus staining buffer solution. A cell fixing solution, a phosphate buffer solution and a lymphocyte separating solution are added, and after dyeing is completed, detection is conducted through a mass spectrometry flow cytometer, and data are collected; according to data analysis, peripheral blood immune cells are divided into CD45 + CD3 + CD4 + T cells, CD45 + CD3 + CD8 + T cells, CD45 + CD3 + CD4 + CD8 + T cells, CD45 + CD3 + CD4 + CD8 + T cells, CD45 + CD19 + B cells, CD45 + CD3 + CD16 + NK cells, CD45 + CD3 + TCRgd + gamma delta T cells, CD45 + CD14 + monocytes, CD45 + HLA-DR + DC cells, neutrophils and basophils through cell membrane surface antibodies, proliferation and differentiation function states of all types of cells are detected through Ki67 and GranzymeB, detection is conducted through a time-of-flight mass spectrometry detector, and the detection result shows that the proliferation and differentiation function states of all the types of cells are detected through a Protein expression information of 46 channels of the same sample can be detected at the same time.

Description

technical field [0001] The invention relates to the technical field of peripheral blood immune cell characteristic atlas research, in particular to a novel coronavirus immunotyping detection method. Background technique [0002] The nucleic acid sequence of the new coronavirus was detected in immune cells such as neutrophils, plasma cells, T cells and natural killer cells, which showed subgenomic transcription characteristics, indicating that the new coronavirus had active transcription and replication in these immune cells , that is to say, immune cells cannot be excluded from the scope of the new coronavirus host cells. [0003] For the analysis of immune cells in patients with new coronavirus pneumonia, detection of changes in peripheral blood immune cell typing can evaluate the immune status of patients with new coronavirus pneumonia, and help new coronary pneumonia drug and clinical research. The existing separation method is to separate peripheral blood Immune cells a...

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Problems solved by technology

[0003] For the analysis of immune cells in patients with new coronavirus pneumonia, detection of changes in peripheral blood immune cell typing can evaluate the immune status of patients with new coronavirus pneumonia, and help new coronary pneumonia drug and clinical research. The existing separation method is to separate peripheral blood Immune cells are labeled with flow cytometry antibodies and separated by traditional flow cytometry. Due to technical limitations such as limited detection channels and overlapping emission spectra of fluorophores in traditional flow cytometry, experiments with more than 12 colors are difficult to carry out, resulting in peripheral blood immunity. The coverage of cell detection is not enough to accurately reflect the characteristic changes of immune cell subsets caused by novel coronavirus pneumonia

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