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Three-dimensional rapid positioning method for particles

A three-dimensional positioning and particle technology, which is applied in image analysis, image enhancement, instruments, etc., can solve the problems of particle number influence, large data volume, and long time consumption, and achieve the effect of reducing hardware cost, compressing memory usage, and improving computing speed

Inactive Publication Date: 2022-03-22
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Laser velocimetry is based on the Doppler effect, which belongs to the single-point velocimetry technology, and cannot give the instantaneous flow velocity data of the whole field; while particle microscopic tracking can realize the observation and monitoring of individual dynamic processes by tracking and matching each particle in the flow field. Transient measurement of the full flow field, but this method is based on the center of mass method and other plane fitting methods for positioning, so the particle position coordinates can only be obtained in the two-dimensional direction, so there is a lack of a dimension in the study of the particle motion law in the three-dimensional space , has great limitations
In the three-dimensional positioning of particles, the existing methods generally obtain the three-dimensional position of particles according to the peak data by performing threshold filtering on the distribution of the three-dimensional light field and fitting the prediction function. The fitting process requires a large amount of data and is time-consuming. Long and affected by the number of particles
In addition, multiple cameras can be used to collect tracer particle images from different viewing angles, and then the three-dimensional tracer particle field can be reconstructed from the two-dimensional images through the optical tomography algorithm. This method does not require relevant light sources, but requires higher Volumetric lighting energy, data storage capacity and computing power also have the problem of time-consuming
[0004] Accordingly, there is an urgent need for a fast and convenient three-dimensional particle positioning method to improve basic problems such as high computing power requirements, extremely long time-consuming, and low efficiency when observing multi-particle samples, and to promote scientific research in related fields

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0035] The embodiment of the present invention provides an experimental result of using a coaxial holographic imaging system for three-dimensional positioning of Escherichia coli. The light source used in the experiment is an LED light source with a wavelength λ=505nm, and the magnification of the holographic imaging system is 40x. Using a black-and-white camera with a pixel number of 1024*1024 and a pixel side length of 6.5 μm, the out-of-focus holographic imaging of the Escherichia coli liquid sample was obtained figure 2 ,in figure 2 Background subtraction has been performed.

[0036] Considering the calculation speed and accuracy, the embodiment of the present invention tracks the bacteria particles within the range of 3.5 μm to 63.5 μm from the imaging surface. According to the pixel side length and magnification, the distance between adjacent reconstructed light intensity images should be 0.1625 μm. Combined with the reconstruction distance, the distance between the r...

Embodiment 2

[0041] The embodiment of the present invention provides an experimental result of three-dimensional positioning of PS plastic balls with a diameter of 0.8 μm dispersed on the same plane using a coaxial holographic imaging system. The light source used in the experiment is an LED light source with a wavelength of λ=450nm. The system magnification is 40x. Using a black-and-white camera with a pixel number of 1024*1024 and a pixel side length of 6.5 μm, the defocused holographic imaging of the plastic bead sample was obtained Figure 4 ,in Figure 4 Background subtraction has been performed.

[0042] In the embodiment of the present invention, tracking is performed within a range of 3.5 μm to 63.5 μm from the imaging plane. According to the pixel side length and magnification, the distance between adjacent reconstructed light intensity images should be 0.1625 μm. Combined with the reconstruction distance, the distance between the reconstructed light intensity surface and the im...

Embodiment 3

[0047] The embodiment of the present invention provides an experimental result of using a coaxial holographic imaging system to perform three-dimensional positioning on a Pseudomonas aeruginosa PAO1 bacterial liquid sample. The light source used in the experiment is an LED light source with a wavelength λ=505nm, and the magnification of the holographic imaging system is 40x . Using a black-and-white camera with a pixel number of 1024*1024 and a pixel side length of 6.5 μm, the defocused holographic imaging of the bacterial liquid sample was obtained Image 6 ,in Image 6 Background subtraction has been performed.

[0048] In the embodiment of the present invention, tracking is performed within a range of 3.5 μm to 63.5 μm from the imaging plane. According to the pixel side length and magnification, the distance between adjacent reconstructed light intensity images should be 0.1625 μm. Combined with the reconstruction distance, the distance between the reconstructed light int...

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Abstract

The invention discloses a three-dimensional rapid positioning method for particles. According to the method, pixel-level particle three-dimensional positioning is realized according to the distribution condition of scattered light intensity of particles in a three-dimensional space and a rapid local maximum search algorithm based on maximum pooling. The invention provides a rapid method for measuring the three-dimensional distribution of the particles in the space, and the method has the advantages of high accuracy and low requirement on the computing power of a computer, and can be widely applied to the three-dimensional distribution and positioning measurement of the particles of bubbles, bacteria, emulsions and solid particles.

Description

technical field [0001] The invention relates to the field of microscopic imaging measurement of particles, in particular to a three-dimensional rapid positioning method for particles, and in particular to the greatly improved positioning speed and accuracy of particles. Background technique [0002] Three-dimensional tracking of particles, that is, precise positioning, is of great significance for studying the dynamic processes of jets, liquid flow fields, emulsion stability, drug particles and microorganisms, etc. The increasing demand of researchers for the observation of particles of micron or even sub-micron size means that there is an urgent need for faster and more accurate positioning of the three-dimensional position of the particle, so as to obtain the technology of the particle's velocity and other motion state parameters. [0003] At present, the common particle dynamic measurement methods include laser velocimetry, particle micro-tracking and so on. Laser veloci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06T7/73G06T19/00G06T19/20
CPCG06T7/73G06T19/003G06T19/20G06T2207/10056G06T2219/2004
Inventor 龚湘君何炳恩张广照
Owner SOUTH CHINA UNIV OF TECH
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