Anti-infection fermented product and preparation method, product and application thereof
A fermented product and anti-infection technology, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, antifungal agents, etc., can solve the problems of no penetration, achieve high bioavailability, efficient preparation methods, and scientific formulations reasonable effect
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Embodiment 1
[0030] The first step is to prepare superfine powder: 8kg of dried black fungus, 6kg of rice bran, 6kg of hawthorn, 6kg of Phellodendron cork, 6kg of black soybean, and 8kg of lotus seed. Crushed into superfine powder with a particle size below 10 μm, set aside.
[0031] The second step is to prepare the seed solution of the fermented strain: the medium contains 0.3% beef extract, 1.0% tryptone, and 0.5% NaCL. The activated Bacillus subtilis, Bifidobacterium lactis, and Lactobacillus plantarum were inoculated at a ratio of 1:1:1, and the inoculum amount was 2%, respectively. They were mixed and cultivated at 36°C for 12 hours to prepare the seed liquid of the fermented strain and set aside.
[0032] The third step prepares the fermentation substrate: according to the weight of the superfine powder obtained in the first step, add 5 times of water to the fermenter, keep it for 5 minutes after boiling, stop heating, cool down, and obtain the fermentation substrate for future use....
Embodiment 2
[0036] The first step is to prepare superfine powder: take 4kg of dried black fungus, 10kg of rice bran, 2kg of hawthorn, 10kg of Phellodendron cork, 2kg of black beans, 12kg of lotus seeds, and 2kg of coix seed. Crushed into superfine powder with a particle size below 10 μm, set aside.
[0037] The second step is to prepare the seed solution of the fermented strain: the medium contains 0.2% beef extract, 1.2% tryptone, and 0.4% NaCL. The activated Bacillus subtilis, Bifidobacterium lactis, and Lactobacillus plantarum were inoculated at a ratio of 1:1:1, and the inoculum amount was 1%, respectively, and mixed cultured at 42°C for 5 hours to prepare the seed liquid of the fermented strain and set aside.
[0038] The third step is to prepare the fermentation substrate: according to the weight of the superfine powder obtained in the first step, add 7 times of water to the fermenter, keep it for 7 minutes after boiling, stop heating, cool down, and obtain the fermentation substrat...
Embodiment 3
[0042] The first step is to prepare superfine powder: take 12kg of dried black fungus, 2kg of rice bran, 10kg of hawthorn, 2kg of Phellodendron cork, 10kg of black beans, 4kg of lotus seeds, and 10kg of coix seed. Crushed into superfine powder with a particle size below 10 μm, set aside.
[0043] The second step is to prepare the seed solution of the fermented strain: the medium contains 0.4% beef extract, 0.8% tryptone, and 0.6% NaCL. The activated Bacillus subtilis, Bifidobacterium lactis, and Lactobacillus plantarum were inoculated at a ratio of 1:1:1, and the inoculum amount was 3%, respectively, and mixed cultured at 30°C for 20 hours to make a seed solution of the fermented strain and set aside.
[0044] The third step prepares the fermentation substrate: according to the weight of the superfine powder obtained in the first step, add 3 times of water into the fermenter, keep it for 3 minutes after boiling, stop heating, cool down, and obtain the fermentation substrate fo...
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