Preparation process of chondroitin sulfate chelated calcium with anti-osteoporosis function

A chondroitin sulfate and anti-osteoporosis technology, which is applied to the functions of food ingredients, drug combinations, bone diseases, etc., can solve the problems of no synergistic effect, loss, and reduced calcium absorption rate of chondroitin sulfate chelated calcium

Active Publication Date: 2022-03-25
MEITEK TECH QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Chondroitin sulfate calcium salt is a relatively new chondroitin raw material, but the calcium in the product has not been chelated in the currently retrieved preparation technologies (as follows Two patents), resulting in the calcium element not being "passivated", most of the chondroitin sulfate calcium is in a non-chelated state, and the calcium ion is in a free state in the product
Therefore, it is easy to lose in the process of absorption and transportation, and the calcium absorption rate decreases.
At the same time, chondroitin sulfate calcium in the chelated state has a special synergistic effect, and has the strengthening effect of anti-osteoarthritis and anti-osteoporosis, while unchelated calcium chondroitin sulfate does not have this effect
Again, the chondroitin sulfate calcium prepared by the prior art does not do deep impurity removal process, resulting in many residual impurities, such as sulfate, chloride, heavy metal residues, miscellaneous proteins and other impurities exceeding the standard, and the effective content of chondroitin sulfate is low
[0006] Chinese patent CN102816809 "a preparation process of chondroitin sulfate calcium salt", discloses a preparation process of chondroitin sulfate calcium salt, including adding water to cook, alkaline hydrolysis Extraction, enzymatic hydrolysis, adsorption and washing, decolorization, precipitation and drying and other process steps can be directly extracted from animal cartilage to prepare calcium chondroitin sulfate, but hydrogen peroxide needs to be added for dec...

Method used

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  • Preparation process of chondroitin sulfate chelated calcium with anti-osteoporosis function
  • Preparation process of chondroitin sulfate chelated calcium with anti-osteoporosis function
  • Preparation process of chondroitin sulfate chelated calcium with anti-osteoporosis function

Examples

Experimental program
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Effect test

Embodiment 2

[0058] Preparation example

Embodiment 3

[0072] 3 Test example: The chondroitin sulfate calcium produced by different processes is comprehensively tested, including CPC content, miscellaneous protein content, sulfate content, chloride content, heavy metal content, calcium, and sodium. The following test data are obtained , where the test basis is as follows:

[0073] 3.1USP Pharmacopoeia: CPC content, miscellaneous protein content, sulfate content, chloride content

[0074] 3.2GB5009.268-2016: Arsenic, lead, mercury, cadmium, calcium, sodium

[0075] 3.3 Calcium chelation rate detection method:

[0076]Mix the 3mg / mL calcium chondroitin sulfate sample with 5mM thoroughly, keep it warm at 37°C for 10min, then add 20mM phosphate buffer (pH 7.8) and react at 37°C for 30min, after the reaction, centrifuge at 4000g for 20min to remove the Calcium phosphate precipitates. At this time, the calcium ions in the solution can remain in a dissolved state without being precipitated by phosphate radicals because of the combinat...

experiment example

[0086] 4 Design of animal test models and comparison of results

[0087] 4.1 Establishment and verification of osteoporosis rat model after ovariectomy

[0088] In this study, an osteoporosis model was established through ovariectomy. 50 female rats (weight 210-230g) were given adaptive feeding for 7 days after receiving the animal room. The rats drank distilled water freely and fed standard feed. The animal room was maintained at a constant temperature of 23±1°C, with 12 hours of light and 12 hours of darkness every day, simulating the alternation of day and night.

[0089] The specific operation of modeling the osteoporosis rat model after ovariectomy is as follows:

[0090] 1) Rats were anesthetized by intraperitoneal injection of sodium pentobarbital (dissolved by ultrasonication at 37° C.) at a dose of 25 mg / kg body weight.

[0091] 2) After complete anesthesia, the abdomen of the rat is upward, the limbs and the mouth are fixed on the rat operating board with cotton th...

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Abstract

The invention belongs to the field of bioactive component extraction, and particularly relates to a preparation process of chondroitin sulfate chelated calcium with an anti-osteoporosis function. The method mainly comprises the following steps: S1, water covering and bone breaking; S2, a chelation reaction 1; S3, free calcium removal; S4, solid-liquid separation, S5, a chelation reaction 2, S6, enzymolysis, S7, free calcium removal, S8, feed liquid washing, S9, vacuum concentration, and S10, drying and packaging. The chelation reaction 1 comprises the following steps: under the conditions of 45-55 DEG C and normal pressure, using a strong reducing agent and a weak base, and under the condition of high-frequency ultrasonic treatment, enabling chondroitin sulfate molecules and calcium ions to be preliminarily combined, so as to achieve the effect of forming a partial chelation state. The invention discloses a special chelating treatment technology, so that chemical bonds between chondroitin sulfate molecules and calcium atoms are changed, stable coordinate bonds are generated, a product is converted into a chelating state from a non-chelating state, and the product has excellent effects in the aspects of calcium absorption and utilization and osteoporosis resistance.

Description

technical field [0001] The invention belongs to the field of bioactive component extraction, and in particular relates to a preparation process of chondroitin sulfate chelated calcium with anti-osteoporosis function. Background technique [0002] Chondroitin sulfate is a kind of glycosaminoglycan, which belongs to acidic mucopolysaccharide, and its molecular weight is generally 20-50KDa. It is widely used in the field of dietary supplements and has a significant effect on joint maintenance and elimination of inflammation. [0003] At present, the commercially available product of chondroitin sulfate is mainly chondroitin sulfate sodium salt (accounting for more than 95% of the market), and the dosage forms included in various national pharmacopoeias are chondroitin sulfate sodium. Sodium chondroitin sulfate only has a certain inhibitory effect on osteoarthritis, because most products only have sodium element (sodium content is about 7%). At the same time, after users take th...

Claims

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Application Information

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IPC IPC(8): C08B37/08A23L33/165A61P19/10
CPCC08B37/0069C08B37/0003A23L33/165A61P19/10A23V2002/00A23V2200/306A23V2300/38A23V2300/48
Inventor 张亮黄周金于明晓宋彦卓赵伟学石佳
Owner MEITEK TECH QINGDAO
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