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Method for carrying out in-situ rapid detection pretreatment on pesticide residues in vegetables and fruits

A pesticide residue and in-situ technology, applied in the preparation of test samples, measuring devices, sampling, etc., can solve the problems of limited application of centrifuges, and achieve significant technological progress, less consumables, and fast processing speed

Pending Publication Date: 2022-03-29
上海市农产品质量安全中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of centrifuges limits its application in the field of in situ rapid analysis

Method used

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  • Method for carrying out in-situ rapid detection pretreatment on pesticide residues in vegetables and fruits
  • Method for carrying out in-situ rapid detection pretreatment on pesticide residues in vegetables and fruits
  • Method for carrying out in-situ rapid detection pretreatment on pesticide residues in vegetables and fruits

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Experimental program
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Effect test

Embodiment 1

[0028] The present invention is a method for in-situ rapid detection and pretreatment of pesticide residues in fruits and vegetables, comprising the following steps:

[0029] 1. Sample preparation

[0030] Chop the vegetable or fruit sample, put it directly into the tissue masher and mash it into a homogenous slurry. (You can also remove the root and chop it, or peel it and chop the skin.)

[0031] Furthermore, the sampling volume of vegetables and fruits shall be carried out in accordance with the provisions of GB / T8855. The sampling location of the sample shall be carried out in accordance with the provisions of GB2763. For small individual samples, all samples are processed after sampling; for large individual basically uniform samples, they can be divided or cut into small pieces on the axis of symmetry or symmetry plane and then processed; for elongated, flat or component content in each part For samples with differences, small pieces can be cut from different parts or...

Embodiment 2

[0046] After the fresh cucumber samples were chopped, they were directly put into a tissue masher to be mashed into a homogenous slurry, and then put into polyethylene bottles. Weigh 10g of sample into a 50mL plastic centrifuge tube, add 20mL of acetonitrile, shake for 2min, filter with filter paper, collect the filtrate, add extraction salt bag and 1 ceramic homogenate to the filtrate, shake vigorously for 1min, and let stand at room temperature for 1 -3min, wait for the solution to separate into layers. Take 1.5mL of supernatant and pass through m-PFC purification column (simple) for one purification, then pass through 0.22μm microporous membrane for gas chromatography determination.

Embodiment 3

[0048] Fresh cucumber samples were peeled, chopped and placed directly into polyethylene bottles. Weigh 10g of cucumber peel into a 50mL plastic centrifuge tube, add 20mL of acetonitrile, shake for 2 minutes, add extraction salt bag and 1 ceramic homogenate, shake vigorously for 1 minute, and let stand at room temperature for 1-3 minutes until the solution is separated. Take 1.5mL of supernatant and pass through m-PFC purification column (simple) for one purification, then pass through 0.22μm microporous membrane for gas chromatography determination.

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Abstract

The invention discloses a method for performing in-situ rapid detection pretreatment on pesticide residues in vegetables and fruits, which comprises the following steps of: preparing a sample: chopping and mashing a vegetable or fruit sample into homogenate, and putting the homogenate into a polyethylene bottle; the extraction method comprises the following steps: weighing a homogeneous sample, adding acetonitrile, shaking, filtering with filter paper, and collecting filtrate; adding an extraction salt bag into the filtrate, vigorously shaking, and standing at room temperature until the solution is layered; and a purification step: sucking the supernatant, carrying out primary purification on the supernatant through a filtration type purification column, and then passing through a microfiltration membrane for determination. Compared with a traditional QuEChERS pretreatment method, a novel rapid filtration method is used as a purification method, and meanwhile, a centrifugation step is omitted by extracting a target object through standing and natural layering after filtration. The method is simple to operate and high in treatment speed, does not need other auxiliary instruments, and is suitable for serving as a pretreatment method for on-site in-situ rapid detection.

Description

technical field [0001] The invention belongs to food detection technology, in particular to a pesticide detection method, in particular to a pre-treatment method for in-situ rapid detection of pesticide residues in fruits and vegetables. Background technique [0002] Pesticide residue detection generally includes two parts: sample pretreatment and instrumental analysis, among which sample pretreatment is a key step that affects the accuracy and precision of test results, and is the research focus of pesticide residue detection. Appropriate pretreatment methods can improve the sensitivity, detection range, precision and accuracy of detection techniques. [0003] At present, the commonly used sample pretreatment methods at home and abroad include solid phase extraction (SPE) [Wu L, Cao ZY, MouRX. Agric Sci Technol, 2017, 18(8): 1526–1530], dispersion liquid-liquid microextraction (DLLME) [Shamsipur M, Yazdanfar N, Ghambarian M. Food Chem, 2016, 204:289–297.], Dispersive Solid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/34G01N1/40G01N30/02G01N30/06
CPCG01N1/286G01N1/34G01N1/405G01N30/02G01N30/06G01N2001/2873G01N2030/062
Inventor 张维谊陈美莲王霞梅博王敏汪弘康沈斯文童金蓉高猛峰
Owner 上海市农产品质量安全中心
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