Zeolite perlite powder gel chitosan resin adsorbent and preparation method thereof, and purification and production method of hyaluronic acid

A technology of chitosan resin and perlite powder, which is applied in the direction of solid adsorbent liquid separation, separation methods, and other chemical processes, can solve the problems of cumbersome process and low removal rate of impurities, and achieve high processing efficiency and strong adsorption Miscellaneous ability, the effect of shortening the process flow

Pending Publication Date: 2022-04-01
INNER MONGOLIA KINGDOMWAY PHARMA LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The purpose of the present invention is to overcome the defects that the process is cumbersome and the removal rate of impurities is low when removing impurities such as thalline, protein, pigment, and metal ions in hyaluronic acid fermentation broth by existing methods, and to provide a A new zeolite perlite powder gel chitosan resin adsorbent and its preparation method and the purification and production method of hyaluronic acid, using the zeolite perlite powder gel chitosan resin adsorbent to treat hyaluronic acid fermentation broth It has the advantages of simple operation, safety, low pollution, low cost and high removal rate of impurities when removing impurities such as bacteria, proteins, pigments, and metal ions

Method used

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  • Zeolite perlite powder gel chitosan resin adsorbent and preparation method thereof, and purification and production method of hyaluronic acid
  • Zeolite perlite powder gel chitosan resin adsorbent and preparation method thereof, and purification and production method of hyaluronic acid
  • Zeolite perlite powder gel chitosan resin adsorbent and preparation method thereof, and purification and production method of hyaluronic acid

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preparation example Construction

[0080] Yet chitosan also has some deficiencies: it is inconvenient to directly use chitosan as a material, and the scope of application is narrow, non-porous and easy to run off, so the present invention needs to make chitosan into gel chitosan sugar resin. Specifically, the preparation method of the gel chitosan resin includes the following steps: S11, dissolving chitosan in a dilute acid solution to obtain a chitosan dilute acid solution; S12, dissolving the metal hydroxide solution with a crosslinking agent Mix evenly to obtain a solidified solution; S13. Slowly add chitosan dilute acid solution to the solidified solution dropwise under stirring conditions, filter after the dropwise addition is completed, wash the wet resin obtained after filtering until it is neutral, and then decolorize. The resin is dried to obtain gel chitosan resin.

[0081] In step S11, specific examples of the dilute acid solution include but are not limited to: at least one of hydrochloric acid sol...

preparation example 1

[0123] This preparation example is used to illustrate the zeolite perlite powder gel chitosan resin adsorbent provided by the present invention and its preparation method.

[0124] (1) Preparation of gel chitosan resin:

[0125] S11. Prepare 1.5 L of a dilute hydrochloric acid solution with a concentration of 1% v / v, add 30 g of chitosan into the dilute hydrochloric acid solution and stir until dissolved to obtain a chitosan dilute acid solution.

[0126] S12. Add 200g of NaOH solid into 500mL of pure water and stir until dissolved to obtain an aqueous sodium hydroxide solution. After cooling, add 200mL of methanol solution and stir evenly to obtain a solidified solution for use.

[0127] S13. Slowly drip the chitosan dilute acid solution obtained in step S11 into the solidified solution obtained in step S12 under the condition of slow stirring with a syringe needle, stir and filter for 10 minutes after the addition is completed, wash the obtained wet resin with water until neut...

preparation example 2

[0132] This preparation example is used to illustrate the zeolite perlite powder gel chitosan resin adsorbent provided by the present invention and its preparation method.

[0133] (1) Preparation of gel chitosan resin:

[0134] S11. Prepare 2 L of dilute acetic acid solution with a concentration of 1% v / v, add 50 g of chitosan into the dilute acetic acid solution and stir until dissolved to obtain a dilute chitosan acid solution.

[0135] S12. Add 250g of NaOH solid into 1000mL of pure water and stir until dissolved to obtain an aqueous sodium hydroxide solution. After cooling, add 300mL of methanol solution and stir evenly to obtain a solidified solution for use.

[0136] S13. Slowly drip the chitosan dilute acid solution obtained in step S11 into the solidified solution obtained in step S12 under the condition of slow stirring with a syringe needle, stir and filter for 10 minutes after the addition is completed, wash the obtained wet resin with water until neutral, and then...

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Abstract

The invention belongs to the technical field of microbial extraction, and relates to a zeolite perlite powder gel chitosan resin adsorbent and a preparation method thereof, and a purification and production method of hyaluronic acid. The zeolite and perlite powder gel chitosan resin adsorbent comprises gel chitosan resin, zeolite powder and perlite powder, wherein the zeolite powder and the perlite powder are loaded on the gel chitosan resin; the preparation method of the gel chitosan resin comprises the following steps: dissolving chitosan in a dilute acid solution to obtain a chitosan dilute acid solution; uniformly mixing the metal hydroxide solution with a cross-linking agent to obtain a curing solution; under the stirring condition, slowly dropwise adding a chitosan dilute acid solution into the curing solution, filtering after dropwise adding is completed, washing wet resin obtained after filtering to be neutral, decolorizing and drying. The zeolite perlite powder gel chitosan resin adsorbent has the advantages of simplicity and convenience in operation, safety, low pollution, low cost and high impurity removal rate when being used for removing thalli, proteins, pigments, metal ions and other impurities in hyaluronic acid fermentation liquor.

Description

technical field [0001] The invention belongs to the technical field of microorganism refining, and in particular relates to a zeolite perlite powder gel chitosan resin adsorbent, a preparation method thereof, and a purification and production method of hyaluronic acid. Background technique [0002] Hyaluronic acid (HA) is also known as hyaluronic acid or hyaluronic acid. Its molecular structure is composed of D-glucuronic acid and N-acetylamino-D-glucose through β-1,4 and β-1,3 glycosidic bonds. connected alternately. With its unique molecular structure and physical and chemical properties, hyaluronic acid shows a variety of important physiological functions in the body, such as lubricating joints, regulating the permeability of blood vessel walls, regulating protein, water and electrolyte diffusion and operation, and promoting wound healing. Hyaluronic acid is also an inherent component in the human body. It is a kind of glucuronic acid, which has no species specificity. I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24B01J20/30B01D15/00B01D36/00C08B37/08
Inventor 王丽吴轶陈忠发蒋四富刘纯旭甄明廖炜程李丹
Owner INNER MONGOLIA KINGDOMWAY PHARMA LTD
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