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Lipid nanogold particle compound and application thereof in delivering ERG and treating encephaledema disease

A technology of gold nanoparticles and composites, applied in gene therapy, genetic material components, pharmaceutical formulations, etc., can solve the problem of less brain edema, achieve the effects of treating brain edema, repairing the blood-brain barrier, and facilitating large-scale production

Pending Publication Date: 2022-04-05
QIANFOSHAN HOSPITAL OF SHANDONG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on the use of gene therapy for cerebral edema

Method used

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  • Lipid nanogold particle compound and application thereof in delivering ERG and treating encephaledema disease
  • Lipid nanogold particle compound and application thereof in delivering ERG and treating encephaledema disease
  • Lipid nanogold particle compound and application thereof in delivering ERG and treating encephaledema disease

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preparation example Construction

[0028] The invention provides a lipid-gold nanoparticle complex for treating cerebral edema by means of gene therapy, the complex is an Ang1-Lipo-AuNP-ERG complex, and the preparation method comprises:

[0029] Add sodium lauryl sulfate to the AuNPs solution for reaction; then add β-mercaptoethylamine for reaction; dialyze and purify the reacted solution, and the dialysate is a weakly acidic citric acid solution; filter after dialysis to obtain Cationic AuNPs solution;

[0030] Add cationic AuNPs solution to ERG plasmid solution and stir overnight to obtain AuNP-ERG solution;

[0031] DSPE-PEG 2000 -MAL and Ang-1 were reacted in solution, purified by dialysis to obtain Ang-DP 2000 ;

[0032] Take soybean lecithin, cholesterol and 2-dioleoyl hydroxypropyl-3-N,N,N-trimethylammonium chloride DOTAP respectively, add them to the methanol solution, heat to dissolve the three, mix well, and cool to room temperature , adding the Ang-DP 2000 Mix well, evaporate methanol to obtain ...

Embodiment 1

[0045] The lipid-gold nanoparticle complex for treating brain edema of the present invention includes the Ang1-Lipo-AuNP-ERG complex. The preparation steps of the complex are specifically:

[0046] (1) Preparation of AuNPs

[0047] The glass instruments used were soaked in aqua regia, washed with distilled water three times and dried for later use; 30 μL of gold tetrachloride solution with a mass fraction of 25.6% and 60 mL of distilled water were added to a 100 mL two-necked bottle, and heated to boiling. Then add 8 mL of 1% trisodium citrate solution, continue heating, the solution turns from light yellow to wine red, stop heating and cool to room temperature after boiling for 20 minutes, and obtain 13±2nm gold nanoparticles. Store at 4°C protected from light.

[0048] (2) Preparation of cationic AuNPs

[0049] Add 0.3 g sodium dodecyl sulfate SDS to the synthesized AuNPs solution and stir for 2 h. Subsequently, 0.5 g of β-mercaptoethylamine was added and stirring was co...

Embodiment 2

[0056] The lipid-gold nanoparticle complex for treating brain edema of the present invention includes the Ang1-Lipo-AuNP-ERG complex. The preparation steps of the complex are specifically:

[0057] (1) Preparation of AuNPs

[0058] All glass instruments used were soaked in aqua regia, washed three times with distilled water, and dried. Add 10 μL of gold tetrachloride solution with a mass fraction of 25.6% and 40 mL of distilled water into a 100 mL two-necked bottle, and heat to boiling. Then add 6 mL of 1% trisodium citrate solution, continue heating, the solution turns from light yellow to wine red, stop heating and cool to room temperature after boiling for 15 minutes, and obtain 13±2nm gold nanoparticles. Store at 4°C protected from light.

[0059] (2) Preparation of cationic AuNPs

[0060] Add 0.1 g sodium dodecyl sulfate SDS to the synthesized AuNPs solution and stir for 1 h. Subsequently, 0.3 g of β-mercaptoethylamine was added and stirring was continued for 2 h. P...

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Abstract

The invention relates to the technical field of gene therapy, relates to a lipid gold nanoparticle compound and application of the lipid gold nanoparticle compound to ERG delivery and encephaledema disease treatment, and provides a lipid gold nanoparticle compound for treating encephaledema through a gene therapy method. The composite material comprises an Ang1-Lipo-AuNP-ERG compound, wherein the Ang1-Lipo-AuNP- The preparation method of the compound comprises the following specific steps: preparation of the AuNPs, preparation of the cation AuNPs, preparation of the AuNP-ERG, and preparation of the Ang1-Lipo-AuNP-ERG. The preparation method has the beneficial effects that the lipid gold nanoparticle compound Ang1-Lipo-AuNP-ERG serving as the gene carrier of the ERG has good biocompatibility and relatively high delivery efficiency. When Ang1-Lipo-AuNP-ERG is taken in by brain endothelial cells, ERG plasmids are expressed in the cells, so that the expression of Claudin-5, VE-Cadherin and ICAM-1 is regulated and controlled, and the effects of repairing the blood brain barrier and treating encephaledema can be achieved.

Description

technical field [0001] The invention relates to the technical field of gene therapy, in particular, it provides a lipid-gold nanoparticle complex for treating cerebral edema by gene therapy. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Cerebral edema is a common clinical dependent disease, which can be secondary or accompanied by a variety of brain diseases, posing a great threat to human health. According to the etiology and pathological mechanism, there are five types of cerebral edema, which are vascular, cytotoxic, osmotic, interstitial, and hydrostatic. Among them, vasogenic cerebral edema is the most common type. The basic pathological mechanism is that the inc...

Claims

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Application Information

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IPC IPC(8): A61K48/00
CPCY02A50/30
Inventor 刘炬蘧文雅李平唐波
Owner QIANFOSHAN HOSPITAL OF SHANDONG
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