Microgel for delivering adipose-derived stem cells and preparation method thereof

Abstract

The invention provides a preparation method of microgel for delivering adipose-derived stem cells, which comprises the following steps: completely dissolving methacryloyl gelatin and a photoinitiator phenyl-2, 4, 6-trimethylbenzoyl lithium phosphonate in phosphate buffer normal saline to obtain a gelatin matrix; taking adipose-derived stem cells transferred to the third generation or after the third generation in the logarithmic phase, putting the adipose-derived stem cells into the gelatin matrix, and enabling the adipose-derived stem cells to suspend in the gelatin matrix; and printing the gelatin matrix mixed with the adipose-derived stem cells by using DLP-3D printing equipment to obtain the microgel carrying the adipose-derived stem cells. According to the prepared microgel bearing the adipose-derived stem cells, the adipose-derived stem cells are evenly distributed in the microgel, the adipose-derived stem cells are basically spherical in the microgel, damage to the adipose-derived stem cells is small, and survival of the adipose-derived stem cells is facilitated. The biocompatibility is good, and the adipose-derived stem cells have no adverse reaction. The microgel contributes to in-vitro adipogenic differentiation of the adipose-derived stem cells, promotes proliferation of the adipose-derived stem cells, and can support in-vitro 3D culture of the adipose-derived stem cells.

Description

technical field [0001] The invention relates to the technical field of adipose stem cell delivery, in particular to a microgel for delivering adipose stem cell and a preparation method thereof. Background technique [0002] Adipose tissue defects can be caused by a variety of reasons, such as aging, trauma, surgery, congenital diseases, etc. The repair of tissue defects is a necessary rehabilitation process for patients. In addition to cosmetic considerations, adipose tissue reconstruction is also indicated in patients with congenital defects, trauma, or surgical resection, such as patients with third-degree burns and women undergoing lumpectomy for breast cancer. [0003] Autologous fat is a commonly used and relatively safe filling material. Autologous fat grafting provides an important means for repairing soft tissue deformities. However, autologous fat grafting has a serious problem of graft absorption, and the long-term survival rate of fat grafts is only about 30%. Ma...

AI Technical Summary

Problems solved by technology

[0004] The earliest and most common method of autologous fat transplantation is granular fat, which can significantly restore tissue volume, but the fat structure is large, and side effects such as ischemic necrosis, calcification, cysts, and uneven filling of adipose tissue are prone to occur; therefore, fat transplantation appeared later The scheme of fat grafts, such as nano-fat, vascular matrix component / vascular matrix component glue, and adipose stem cells obtained through different steps such as centrifugation, emulsification, filtration, and enzymatic hydrolysis, etc., but the absorption phenomenon is more serious after filling, and the restoration of tissue volume less effective than structural fat

Additionally, high fat absorption rates require repeated procedures, resulting in lower patient satisfaction

[0005] Materials such as collagen and hyaluronic acid can be used to fill adipose tissue defects, please refer to Liu X, Tao J, Liu J, et al. 3D printing enabled customization of functional microgels[J].ACSapplied materials&interfaces,2019,11(13 ):12209-12215 and Major G S, Simcock JW, Woodfield T B F, et al. Overcoming functional challenges in autologous and engineered fat grafting trends[J].Trends in Biotechnology, 2021, but rapid absorption and inflammatory responses and limited their application , in addition, commercial products are very expensive and require repeated injections

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