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Method for simultaneously detecting multiple amino acids

An amino acid and histidine technology, which can be used in measuring devices, instruments, scientific instruments, etc., can solve the problem of not being able to separate and measure multiple amino acid components at the same time, and achieve the effects of high sensitivity, good precision, and simple gradient elution procedure.

Active Publication Date: 2022-04-12
HANGZHOU ZHUYANGXIN PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can effectively separate and measure Apremilast from known impurities and unknown impurities, but this method cannot simultaneously separate and measure multiple amino acid components

Method used

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  • Method for simultaneously detecting multiple amino acids
  • Method for simultaneously detecting multiple amino acids
  • Method for simultaneously detecting multiple amino acids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1.1 Instrument

[0028] 1260 high-performance liquid chromatography (Agilent Technologies Co., Ltd.), LC-2030C 3D plus high-performance liquid chromatography (Shimadzu Enterprise Management (China) Co., Ltd.), XS205DU electronic balance (Mettler-Toledo International Trading (Shanghai) limited company)

[0029] 1.2 Reagents and reagents

[0030] Compound amino acid capsules (purchased from Queisser Pharma of Germany's Queiss Pharmaceutical Industry, trade name Doppel Herz TM , which contains L-tryptophan, L-tyrosine, L-histidine, L-phenylalanine and other amino acids), L-tryptophan, L-tyrosine, L-histidine , L-phenylalanine, Boc-histidine, N-acetyl-L-leucine, PHT-glutamic acid, 3,5-dibromo-L-tyrosine, S-phenyl-L- Cysteine, O-benzyl-L-threonine reference substance, phosphoric acid (chromatographically pure, Aladdin), acetonitrile (chromatographically pure, Merck).

[0031] 1.3 Detection method

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Abstract

The invention belongs to the field of pharmaceutical analysis, and particularly relates to a method for simultaneously detecting multiple amino acids. In the HPLC method, a mobile phase A is a 0.05-0.2% phosphoric acid aqueous solution, a mobile phase B is acetonitrile, and the gradient elution method is as follows: 0 min, 96-100% of the mobile phase A and 0-4% of the mobile phase B; the mobile phase A accounts for 96-100%, and the mobile phase B accounts for 0-4%; the mobile phase A comprises 48 to 52 percent of mobile phase A and 48 to 52 percent of mobile phase B; the mobile phase A accounts for 48-52%, and the mobile phase B accounts for 48-52%. The detection method is sensitive, high in accuracy, good in repeatability and simple and convenient in sample treatment, and has wide popularization significance.

Description

technical field [0001] The invention belongs to the field of drug analysis, in particular to a method for simultaneously detecting multiple amino acids. Background technique [0002] At present, for the detection of amino acids, the commonly used methods are derivatization method, potential sensing method and capillary electrophoresis method, etc. These methods bring certain difficulties to daily detection operations due to cumbersome sample processing and poor reproducibility. , poor popularity and not suitable for the detection of trace elements. Some commercially available amino acid products are obtained through chemical synthesis, and there will be amino acid derivatives, intermediates and degradation impurities in the product. In order to control the quality of the product, It needs to be monitored. For example, N-acetyl-L-leucine is synthesized by the reaction of L-leucine and acetic anhydride. As a biochemical reagent, it can be used in the synthesis of chemical raw...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/34G01N30/02
Inventor 马雯霞郭艳超唐建飞戚兰君谢红瑜吴倩倩范仲姝陈宣其
Owner HANGZHOU ZHUYANGXIN PHARMA
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