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Oxidase-loaded microneedle for promoting wound healing of diabetic patient and preparation method of oxidase-loaded microneedle

A technology for wound healing and diabetic patients, which is applied in the field of oxidase-carrying microneedles and its preparation to promote wound healing of diabetic patients. To improve the oxidative microenvironment, protect cell damage and apoptosis, and reduce the level of oxidative stress

Active Publication Date: 2022-04-22
THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Through the above analysis, the existing problems and defects of the prior art are: natural biological antioxidant enzymes have few sources, high preparation costs, and easy inactivation, and the therapeutic effect of single functional materials or drugs on diabetic skin wounds with complex pathological mechanisms limited
[0008] The difficulty in solving the above problems and defects is: due to the complexity of the pathological mechanism of diabetic skin trauma, it lacks effective means in clinical treatment
According to its main pathological mechanism, choosing appropriate drugs and carriers to promote diabetic skin wound healing is a huge challenge

Method used

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  • Oxidase-loaded microneedle for promoting wound healing of diabetic patient and preparation method of oxidase-loaded microneedle
  • Oxidase-loaded microneedle for promoting wound healing of diabetic patient and preparation method of oxidase-loaded microneedle
  • Oxidase-loaded microneedle for promoting wound healing of diabetic patient and preparation method of oxidase-loaded microneedle

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preparation example Construction

[0041] Such as figure 1 As shown, the preparation method of the oxidase-carrying microneedle provided by the embodiment of the present invention to promote diabetic wound healing comprises the following steps:

[0042] S101, dissolve 15w / v% SilMA in 15mL deionized water and stir to dissolve; under the condition of avoiding light, add 1w / v% photoinitiator HMPP and continue stirring to obtain a mixed solution A;

[0043] S102, take 5mL mixed solution A, add PBZs to make the concentration 100-500ug / ml, add VEGF solution to make the concentration 3-10ng / ml, and get mixed solution B; take 5mL mixed solution A, add polymyxin The solution has a concentration of 3-5 μg / ml to obtain a mixed solution C;

[0044] S103, add the mixed solution B into the microneedle mold, place it in a vacuum environment for 30s, absorb the excess liquid on the surface, and place it at a strength of 3.5mJ / cm 2 Cross-linking under high-intensity ultraviolet light for 5 minutes to obtain the tip part of th...

Embodiment 1

[0048] The invention uses the biological activity of PBZs to efficiently remove active oxygen, the angiogenesis-promoting effect of vascular endothelial growth factor (VEGF), and the antibacterial ability of polymyxin (Polymyxin), using SilMA composite hydrogel microneedles as the carrier , Constructing PBZs-based multifunctional nanocomposite bioactive materials for diabetic skin wound treatment.

[0049] The invention provides a PBZs-loaded multifunctional nanocomposite hydrogel microneedle for the treatment of diabetic skin wounds, and the invention provides more options for the clinical treatment of diabetic skin wounds.

[0050] The invention provides the following technical solutions:

[0051] First, PBZs and VEGF were mixed with SilMA composite hydrogel to prepare the tip of the microneedle, and then polymyxin was mixed with SilMA composite hydrogel to prepare the base layer of the microneedle to obtain a multifunctional nanocomposite hydrogel microneedle. When the mic...

Embodiment 2

[0054] 1. Preparation process of PBZs composite microneedles

[0055] Dissolve 15w / v% SilMA in 15mL deionized water and stir to dissolve. Under the condition of avoiding light, add 1w / v% photoinitiator (HMPP) and continue stirring to obtain mixed solution A. Take 5mL mixed solution A, add PBZs to make the concentration 100-500ug / ml, add VEGF solution to make the concentration 3-10ng / ml, and get mixed solution B. Then take 5 mL of mixed solution A, add polymyxin solution so that its concentration is 3-5 μg / ml, and obtain mixed solution C. Add the mixed solution B into the microneedle mold, place it in a vacuum environment for 30s, absorb the excess liquid on the surface, and place it at a strength of 3.5mJ / cm 2 Cross-linking under high-intensity ultraviolet light for 5 minutes can obtain the tip part of the composite microneedle. Then add the mixed solution C into the microneedle mold and place it at a strength of 3.5mJ / cm 2 Cross-linking under strong ultraviolet light for ...

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Abstract

The invention belongs to the technical field of biological materials and biological medicines, and discloses an oxidase-loaded microneedle for promoting wound healing of diabetics and a preparation method thereof.The preparation method comprises the steps that SilMA is dissolved in deionized water, and a photoinitiator HMPP is added under the dark condition to obtain a mixed solution A; adding PBZs and VEGF solution into the mixed solution A to obtain a mixed solution B; adding the mixed solution A into a polymyxin solution to obtain a mixed solution C; and respectively adding the mixed solution B and the mixed solution C into a microneedle mold, carrying out crosslinking under ultraviolet light, and separating the mold to obtain the composite microneedle. The PBZs and the VEGF are creatively loaded into the SilMA composite hydrogel microneedle to treat diabetic skin wounds, the oxidative stress level of diabetic skin wound tissues can be effectively relieved, a proper oxidation microenvironment is provided for the VEGF to play a role in promoting angiogenesis, and healing of the diabetic skin wounds can be better promoted.

Description

technical field [0001] The invention belongs to the technical field of biomaterials and biomedicine, and in particular relates to an oxidase-carrying microneedle for promoting wound healing of diabetic patients and a preparation method thereof. Background technique [0002] At present, diabetes is a chronic metabolic disorder in which multiple organs of the body are affected due to defects in insulin secretion and (or) action, causing hyperglycemia and metabolic disorders in the body. At present, the incidence of diabetes is increasing year by year, and it has become a worldwide health problem. Skin wounds in diabetic patients are difficult to heal and are prone to infection. The skin trauma of diabetic patients caused by various reasons has become a public health issue of concern all over the world, which brings great economic and social burdens to patients' families, medical and health systems and society. Therefore, the problem of promoting diabetic skin wound healing n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/70A61K47/42A61K38/18A61K33/26A61K38/12A61P17/02A61P3/10A61P31/04A61M37/00
CPCA61K9/7023A61K9/0021A61K47/42A61K38/1866A61K33/26A61K38/12A61P17/02A61P3/10A61P31/04A61M37/0015A61M2037/0046A61M2037/0053A61K2300/00Y02A50/30
Inventor 金萍管高鹏张勤刘婕肖祚庥
Owner THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
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