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Fermentation medium for increasing apramycin fermentation unit and application thereof

A technology for fermentation medium and seeds, applied in the directions of fermentation, microorganism-based methods, and adding compounds to stimulate growth, etc., can solve the problems of low titer of apramycin, achieve important industrial value, reduce production costs of enterprises, Yield improvement effect

Pending Publication Date: 2022-04-22
SHANGHAI INST OF PHARMA IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the technical problem of low titer of producing apramycin in the prior art, the invention provides a fermentation medium and its application

Method used

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  • Fermentation medium for increasing apramycin fermentation unit and application thereof
  • Fermentation medium for increasing apramycin fermentation unit and application thereof
  • Fermentation medium for increasing apramycin fermentation unit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 Carbon source content experiment in fermentation medium

[0055] Using the basic fermentation medium formulation as a control, the amount of glucose was explored under the condition that the nitrogen source, inorganic salts and other components remained unchanged. Shaker bottle fermentation for 6 days, using high performance liquid phase measurement fermentation unit.

[0056]

[0057] According to the results of the above experiments, the optimized formula is (the amount of addition is g / 100mL): glucose 6.0, cold bean powder 4.0, dolphin peptone 1.0, ammonium chloride 0.5, magnesium sulfate 0.4, manganese chloride 0.03, zinc sulfate 0.003, ferrous sulfate 0.005, calcium carbonate 0.5, soybean oil 1.0, tap water 100ml, pH adjusted to 7.0-7.1 before sterilization.

Embodiment 2

[0058] Example 2 Carbon source substitution experiment in fermentation medium

[0059] Taking the optimized medium formulation of Example 1 as a control, under the condition that other components such as nitrogen sources, inorganic salts and other components remain unchanged, glucose is partially replaced by 2% other carbon sources.

[0060] Carbon source type glucose cornmeal cornstarch Maltodextrin maltose cane sugar Potency (g / L) 4.58 4.85 2.47 5.17 3.30 4.30

[0061] The optimized formula is (added at g / 100mL): glucose 4.0, maltodextrin 2.0, cold bean flour 4.0, dolphin peptone 1.0, ammonium chloride 0.5, magnesium sulfate 0.4, manganese chloride 0.03, zinc sulfate 0.003, ferrous sulfate 0.005, calcium carbonate 0.5, soybean oil 1.0, tap water 100ml, pH adjusted to 7.0-7.1 before sterilization.

Embodiment 3

[0063] Taking the optimized medium formulation of Example 2 as a control, under the condition that other components such as nitrogen sources, inorganic salts and other components remain unchanged, glucose is partially replaced by 1% of other carbon sources.

[0064] Carbon source type glucose cornmeal cornstarch Maltodextrin maltose cane sugar Potency (g / L) 5.17 5.58 3.46 5.27 4.29 4.65

[0065]The optimized formula is (added at g / 100mL): glucose 3.0, maltodextrin 2.0, corn flour 1.0, cold bean flour 4.0, dolphin peptone 1.0, ammonium chloride 0.5, magnesium sulfate 0.4, manganese chloride 0.03, zinc sulfate 0.003, ferrous sulfate 0.005, calcium carbonate 0.5, soybean oil 1.0, tap water 100ml, pH adjusted to 7.0-7.1 before sterilization.

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Abstract

The invention discloses a fermentation medium and application thereof. The fermentation medium comprises: (1) 7% of a carbon source, the carbon source comprising soybean oil and one or more of glucose, maltodextrin and corn flour; (2) 5.5% of a nitrogen source, wherein the nitrogen source is selected from one or more of cold bean powder, peanut powder, dolphin peptone and yeast powder YP600; and (3) 0.9-1.1% of inorganic salt which is selected from one or more of ammonium chloride, magnesium sulfate, manganese chloride, zinc sulfate, ferrous sulfate and calcium carbonate; and the balance is water, and the percent is the mass volume percent g / 100mL of the fermentation culture medium. The fermentation medium can improve the fermentation unit of apramycin from 5.3 g / L in the prior art to at least 6.25 g / L, and the fermentation unit is improved by 18%.

Description

Technical field [0001] The present invention belongs to the field of industrial microbial technology, relates to a fermentation medium and application thereof to improve ampramycin fermentation units. Background [0002] Ampcomycin is a widely used broad-spectrum antibiotic, the current method of improving amphotericin is mainly to carry out genetic engineering of the strain and breeding, but the effect is not obvious, the present invention by optimizing the fermentation medium to make ampromycin fermentation titer significantly improved. [0003] Tian Wei et al. (Tian Wei. The study of ampramycin producing bacteria [D].Shenyang Pharmaceutical University, 2002) optimized amphotericin fermentation medium by mean design experiments, and finally determined that the optimal fermentation conditions of ampramycin were: glucose 4.5g / L, hydrolyzed starch 3.0g / L, soybean cake powder 2.0g / L, glycerin 0.3g / L, ammonium sulfate 0.15g / L, yeast powder 0.1g / L, peanut cake powder 3.5g / L, NA 0.4g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/20C12P19/60C12P19/26C12R1/465
CPCC12N1/38C12N1/20C12P19/60C12P19/26
Inventor 李继安林惠敏郭瑞玲孟宪纬张建斌李亚军詹佳弘杨子艳赵明芳袁笑端
Owner SHANGHAI INST OF PHARMA IND
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