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DNA (deoxyribonucleic acid) nano system for tumor targeting as well as preparation method and application of DNA nano system

A tumor-targeting, nanotechnology, applied in the field of tumor-targeted DNA nanosystems and its preparation, can solve the problems of limited nanosystems, low targeting efficiency, and poor therapeutic effects, and achieve expanded application range and easy penetration , combined with fast effects

Pending Publication Date: 2022-04-26
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the nanosystems for tumor targeting provided in the prior art are still very limited, and there are generally problems such as low targeting efficiency, poor therapeutic effect, and obvious drug resistance, and for some tumors that lack specific targets of tumors helpless

Method used

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  • DNA (deoxyribonucleic acid) nano system for tumor targeting as well as preparation method and application of DNA nano system
  • DNA (deoxyribonucleic acid) nano system for tumor targeting as well as preparation method and application of DNA nano system
  • DNA (deoxyribonucleic acid) nano system for tumor targeting as well as preparation method and application of DNA nano system

Examples

Experimental program
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Embodiment 1

[0064] This embodiment provides a DNA nanosystem for tumor targeting, the preparation method of which is as follows:

[0065] (1) Preparation of two-dimensional DNA nanosheets

[0066] A long scaffold chain is mixed with the pre-designed stapled short chain and the upper and lower capture chains, cooled by PCR program, and assembled according to the principle of base complementary pairing to obtain a two-dimensional DNA nanosheet structure. The scaffold chain is the M13 genome, and the staple short chain is a nucleic acid sequence complementary to the scaffold chain. The upper and lower capture strands are used to hybridize two different functional groups, respectively.

[0067] The specific operation is: mix the scaffold chain, staple short chain, upper capture chain and lower capture chain with TAE-Mg at a ratio of 1:5:5:5 2+ In the solution (Tris-Base, 40mM; acetic acid, 20mM; EDTA, 2mM; magnesium acetate, 12.5mM; pH 8.0), the temperature was raised to 95°C, and then slow...

Embodiment 2

[0079] This embodiment provides a DNA nanosystem for tumor targeting. The difference between its preparation method and Example 1 is that the photosensitizer Ce6 in step (4) is replaced by the fluorescent dye indocyanine green (ICG), thereby To obtain the SA-ICG coupling product, the specific operation is as follows:

[0080] SA was dissolved in PBS to make a 200 μM stock solution, and NHS-activated indocyanine green was dissolved in DMSO to make a 6 mM ICG-NHS stock solution. Mix 100 μL SA stock solution with 20 μL ICG-NHS stock solution and shake at 25° C. for 2 h. After the reaction was completed, excess ICG was removed using a desalting column with a molecular weight cut-off of 40 KDa to obtain a SA-ICG coupling product.

[0081] Refer to Example 1 for other steps.

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Abstract

The invention provides a DNA nano system for tumor targeting as well as a preparation method and application of the DNA nano system. The DNA nano system for tumor targeting comprises a DNA nano sheet layer, a single-stranded DNA coupled with a low-pH insertion peptide, a biotin-modified single-stranded DNA and a streptavidin-functional molecule conjugate, wherein the DNA nanosheet layer, the single-stranded DNA coupled with the low-pH insertion peptide and the biotin-modified single-stranded DNA are combined in a base complementary pairing manner to form a difunctional DNA nanosheet layer; the streptavidin-functional molecule conjugate is combined with the difunctional DNA nanosheet layer through the specific recognition effect of streptavidin and biotin, so as to form the DNA nano system. The DNA nano-system provided by the invention has good biological safety and low toxic and side effects, and can be used as a drug delivery system, the drug delivery system can construct artificial targets on the surfaces of tumor cells, the purpose of targeting tumors lacking specific targets is achieved, and the DNA nano-system has potential application value in tumor treatment and diagnosis.

Description

technical field [0001] The invention belongs to the field of nanomedicine, relates to a DNA nano system and its preparation method and application, in particular to a DNA nano system for tumor targeting and its preparation method and application. Background technique [0002] In recent years, malignant tumor (cancer) has become one of the major diseases that endanger human health. At present, the clinical treatment of cancer mainly relies on radiotherapy, chemotherapy and surgical treatment. These methods alleviate the pain of patients to a certain extent, but they all have certain limitations and the cure rate is low. With the continuous development of nanotechnology and the continuous integration of nanotechnology and modern medicine, an interdisciplinary subject such as nanomedicine has been formed, and its potential applications involve the diagnosis, monitoring and treatment of diseases. The use of nano-carriers to load chemotherapy drugs can reduce the damage of chemo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K47/64A61K47/42A61K47/26A61P35/00B82Y5/00B82Y40/00A61K49/00
CPCA61K41/0071A61K41/0052A61K47/64A61K47/42A61K47/26A61P35/00B82Y5/00B82Y40/00A61K49/0034A61K49/0052A61K49/0056
Inventor 丁宝全仵宇帅蒋乔
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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