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Strain of saccharomycopsis fibuligera for producing 3-methylthio propanol as well as culture method and application of strain of saccharomycopsis fibuligera

A technology of methylthiopropanol and buckle capsule lamination, which is applied in the field of microorganisms to achieve the effects of increasing production, optimizing ingredients and culture conditions

Pending Publication Date: 2022-04-26
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problem that natural green 3-methylthiopropanol cannot meet market demand, and the practical application of increasing the content of 3-methylthiopropanol in traditional brewed food sesame-flavored liquor and improving the quality of liquor, the purpose of the present invention is to provide a A new high-yielding 3-methylthiopropanol strain isolated from liquor koji, named Y1402, and provides the medium, culture method and application of Y1402 for producing 3-methylthiopropanol

Method used

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  • Strain of saccharomycopsis fibuligera for producing 3-methylthio propanol as well as culture method and application of strain of saccharomycopsis fibuligera
  • Strain of saccharomycopsis fibuligera for producing 3-methylthio propanol as well as culture method and application of strain of saccharomycopsis fibuligera
  • Strain of saccharomycopsis fibuligera for producing 3-methylthio propanol as well as culture method and application of strain of saccharomycopsis fibuligera

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Separation of Saccharomyces fornicata Y1402

[0045] Yeast strains were isolated from Daqu powder using traditional microbial isolation methods, and the specific method was as follows: Take 1 g of crushed and mixed Daqu powder and dilute it 10 times with sterile water. In a sterile environment, the bacterial solution gradient was diluted to 10 -5 、10 -6 and 10 -7 . Spread 0.1 mL of each serial dilution on a YPD plate and incubate at 30°C for 2 days. Pick a single colony with white protrusions and opaque yeast morphology, and streak it on the YPD plate to obtain pure yeast, and save it in a glycerol tube for future use.

[0046] The yeast screened from Daqu was inoculated in YPD medium and activated at 28°C and 180 r / min for 24 h. According to the inoculum amount of 0.2%, inoculate the seed liquid of the three activated yeast strains into the sterilized 3-methylthiopropanol transformation medium, and put them in a shaker with a temperature of 30 °C and a r...

Embodiment 2

[0050] Example 2 Identification of Saccharomyces fornicata Y1402

[0051] Gained vesicle complex membrane yeast ( Saccharomycopsis fibuligera ) The identification of Y1402 includes the following steps:

[0052] Step 1: Morphological Characterization

[0053] In order to identify the yeast strains involved, the following morphological characteristics were observed:

[0054] Yeast colony morphology observation: Inoculate the yeast in YPD liquid medium and culture for 24-48 h, inoculate the activated strain into the WL differential medium with a three-section line, and place it in a constant temperature and humidity incubator at 30°C for static After culturing for 48 h, the colony morphology was observed at the end of the culture. The colony morphology of strain Y1402 on WL differential medium is as follows: figure 1 As shown in a, the colony is white, with circular protrusions, and the colony is small. The color of the medium where the strain grows changes from blue-green t...

Embodiment 4

[0096] Example 4 Growth characteristics of yeast Y1402

[0097] The present invention relates to the growth characteristics of the vesicle complex membrane yeast, which is characterized in that the steps are as follows:

[0098] Step 1: Temperature Tolerance

[0099] Using YPD medium as the base medium, the activated bacterial solution was inoculated into YPD medium at an inoculum amount of 0.2%, at temperatures of 20°C, 25°C, 30°C, 35°C, 40°C, 45°C, OD was measured by turbidity method after cultured at 50°C and 180 r / min for 48 hours 560 . Turbidity method: Put the fermentation broth in a cuvette, use a spectrophotometer to detect the optical density of the fermentation broth at a wavelength of 560nm, and the measured OD value can be used to reflect the concentration of bacteria. The result is as image 3 As shown in a, the strain involved in the present invention cannot grow when the temperature is higher than 40°C, and its optimum growth temperature is 25°C.

[0100] S...

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Abstract

The invention belongs to the technical field of microorganisms, and provides a saccharomycopsis fibuligera strain with high yield of 3-methylthio propanol, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.23667. The candida endothelium can be used for producing the 3-methylthio propanol, the yield can reach 3.16 g / L, the ethanol tolerance is high, and the candida endothelium can be applied to the industries of wine brewing, tobacco shred fermentation, seasonings and food additives.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a yeast strain producing 3-methylthiopropanol and its cultivation method and application. Background technique [0002] Baijiu is China's national liquor, and there are currently twelve flavor types. Sesame Fragrant Baijiu is an innovative scented wine, which has the characteristics of "prominent sesame aroma, harmonious flavors, plump and delicate, and long aftertaste". The uniqueness of flavor substances creates differences in the style of wine body. In GB / T 20824-2007 "Sesame-flavored Liquor", 3-methylthiopropanol is identified as the characteristic flavor substance of sesame-flavored liquor, and the alcohol content is specified The mass concentration of 3-methylthiopropanol must be greater than 0.5 mg / L, and the mass concentration of low-alcohol alcohol must be greater than 0.4 mg / L. [0003] 3-Methylthiopropanol (molecular formula C 4 h 10 OS), also known as...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P11/00C12G3/021A23L27/00A23L29/00A23L31/10C12R1/645
CPCC12N1/16C12P11/00C12G3/021A23L29/065A23L31/10A23L27/00A23V2002/00A23V2250/76
Inventor 范光森张玉姣成柳洁孙宝国马景浩滕超杨然詹夏晖
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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