Unlock instant, AI-driven research and patent intelligence for your innovation.

Microbial complex microbial inoculant for sugarcane planting and preparation method of microbial complex microbial inoculant

A compound bacterial agent and microbial technology, which is applied in the field of sugarcane planted microbial compound bacterial agent and its preparation, can solve the problems of low utilization rate of equipment, low sugar production, short pressing period, etc., and achieve the goal of promoting sugarcane yield and sugar content Elevate, promote the accumulation of sucrose, promote the effect of growth

Pending Publication Date: 2022-05-06
SHENZHEN AGRECOE BIOTECHNOLOGY CO LTD
View PDF13 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The amount of cane crushed is the basis, which is related to the profit of 10,000 tons of cane. However, only with high sucrose content and no certain amount of raw cane, the crushing period is short, the sugar production is small, the utilization rate of equipment is low, and the economic benefits are not high.
And although there is a certain amount of raw material cane but its sucrose content is low, the sugar yield is low, the cost is high, and the economic benefit is not great.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microbial complex microbial inoculant for sugarcane planting and preparation method of microbial complex microbial inoculant
  • Microbial complex microbial inoculant for sugarcane planting and preparation method of microbial complex microbial inoculant
  • Microbial complex microbial inoculant for sugarcane planting and preparation method of microbial complex microbial inoculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] This embodiment discloses a kind of microbial composite bacteria agent for sugarcane plantation, including the following raw materials:

[0051] 3.5kg composite bacterial agent A; 5.0kg composite bacterial agent B; 1.5kg regulator; regulator is ethephon.

[0052] Composite bacterial agent A comprises 55wt% of bacillus licheniformis bacterial suspension, 25wt% of cercospora bacterial suspension and 20wt% of alginic acid; The preparation method of composite microbial agent A is:

[0053] S1-1, after solid activation and culture of Bacillus licheniformis and Cercospora strains respectively, the prepared concentration is 1-3×10 8 Bacillus licheniformis suspension seed liquid and Cercospora bacterial suspension seed liquid of CFU / ml;

[0054] S1-2, inoculate the Bacillus licheniformis suspension seed solution to the PDA medium with an inoculum amount of 5%, carry out single-plant shake flask fermentation culture on a shaker, the culture temperature is 32°C, and the shaker o...

Embodiment 2

[0065] This embodiment discloses a kind of microbial composite bacteria agent for sugarcane plantation, including the following raw materials:

[0066] 4.0kg composite bacterial agent A; 5.5kg composite bacterial agent B; 0.5kg regulator; regulator is ethephon.

[0067] The preparation method of composite microbial agent A is:

[0068] S1-1, after solid activation and culture of Bacillus licheniformis and Cercospora strains respectively, the prepared concentration is 1-3×10 8 Bacillus licheniformis suspension seed liquid and Cercospora bacterial suspension seed liquid of CFU / ml;

[0069] S1-2, inoculate the Bacillus licheniformis suspension seed solution to the PDA medium with an inoculum amount of 7%, and carry out a single-plant shake flask fermentation culture on a shaker, the culture temperature is 35 ° C, and the shaker oscillation speed is 170 rpm. The time is 73h to obtain a suspension of Bacillus licheniformis;

[0070] S1-3, inoculate the Cercospora suspension seed...

Embodiment 3-6

[0081] A microbial composite bacterial agent for sugarcane planting. The difference between this embodiment and Example 1 is that the content of each bacterial suspension in the composite bacterial agent A and B is different and the selected substances of the regulator are different. See Table 1 for details.

[0082] Table 1

[0083]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a microbial complex microbial inoculant for sugarcane planting and a preparation method thereof. The microbial complex microbial inoculant comprises the following components in percentage by mass: 35-40wt% of a complex microbial inoculant A; 50-55wt% of a complex microbial inoculant B; 5-15 wt% of a conditioning agent; the complex microbial inoculant A is prepared from 55 to 60 weight percent of bacillus licheniformis bacterial suspension, 25 to 30 weight percent of cephalosporium bacterial suspension and 10 to 20 weight percent of alginic acid; and the complex microbial inoculant B at least comprises 45-50 wt% of a bacillus mucilaginosus bacterial suspension, 25-30 wt% of a photosynthetic bacterium bacterial suspension and 16-30 wt% of a silicate bacterium bacterial suspension. The method has the effect of improving the yield and the sugar content of the sugarcane.

Description

technical field [0001] The present application relates to the field of microbial composite bacterial agents, in particular to a microbial composite bacterial agent for sugarcane planting and a preparation method thereof. Background technique [0002] Sugarcane is a temperate and tropical crop, and sucrose is one of the final products of photosynthesis in sugarcane, and it is also the main form of transport and distribution of photosynthetic products in most plants. "Double high" sugarcane refers to sugarcane with high yield and high sugar content. As a raw material for making sucrose and refining ethanol, sugarcane has high economic benefits and has been widely planted and promoted in southern my country. [0003] Sugarcane is the raw material of the sugar industry, and the sugar content of sugarcane is the source of the economic benefits of the sugar industry. The economic benefits of the sugar industry refer to the three main factors, the amount of cane crushed, the suga...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14A01G22/55C05F11/08C12R1/645C12R1/10C12R1/07C12R1/01
CPCC12N1/20C12N1/14A01G22/55C05F11/08
Inventor 王剑英孔宗耀
Owner SHENZHEN AGRECOE BIOTECHNOLOGY CO LTD