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Application of fructose-1, 6-dicalcium phosphate to improvement of growth-promoting bacteria in plant rhizosphere colonization

A technology of calcium diphosphate salt and calcium diphosphate, which is applied in the biological field to achieve the effect of significant chemotaxis and group migration, the formation of promotion, and the wide application prospect.

Pending Publication Date: 2022-05-20
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the study of promoting the colonization of growth-promoting bacteria in the plant rhizosphere as a signal molecule

Method used

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  • Application of fructose-1, 6-dicalcium phosphate to improvement of growth-promoting bacteria in plant rhizosphere colonization
  • Application of fructose-1, 6-dicalcium phosphate to improvement of growth-promoting bacteria in plant rhizosphere colonization
  • Application of fructose-1, 6-dicalcium phosphate to improvement of growth-promoting bacteria in plant rhizosphere colonization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 The effect of fructose-1,6-diphosphate calcium salt (FB) on the formation of rhizobacteria NRCB002, NRCB005, NRCB010 and Lv5A biofilm.

[0027] (1) Experimental method

[0028] Connect each bacterium from the plate to a 50ml centrifuge tube, cultivate at 30°C and 200rpm for 12 hours, draw 1% of the inoculum into a 250 Erlenmeyer flask filled with 50ml LB culture solution, and cultivate overnight.

[0029] Draw 20ml of the bacterial suspension and centrifuge at 5000rpm for 5min (50ml centrifuge tube). The bacterial cells were collected by washing twice with normal saline, and the volume was adjusted to about 1.2 with K's medium. Add 37.5 μL of bacterial solution and 112.5 μL of K’s medium with different FB concentrations (0, 0.1, 0.5, 1 g / L) to the 96-well plastic culture plate in sequence, set up 8 parallels for each treatment, and use equal amounts of sterile culture As a blank control group, cultured at 28°C for 48 hours.

[0030] After culturing for 48 h...

Embodiment 2

[0037] Example 2 Effect of fructose-1,6-diphosphate calcium salt (FB) on the swimming diameter of rhizosphere growth-promoting bacteria NRCB002, NRCB005, and NRCB010.

[0038] (1) Experimental method

[0039] Swimming medium: Each 1L contains tryptone 10g, sodium chloride 10g, yeast powder 5g and agar powder 5g.

[0040] NBNS liquid medium was used to inoculate overnight activated seed solutions of strains NRCB002, NRCB005, and NRCB010. Cultivate to mid-logarithmic phase (OD600=1.0) at 30°C and 200 rpm. Add 100 μl of 0.1 g / L FB solution to the Swimming plate and spread evenly. Place a circular sterile paper disc with a diameter of 0.5 cm in the center of the plate, suck 5 μl of bacterial solution slowly onto the center of the disc with a pipette tip, let it stand for ten minutes in a sterile environment, and gently move the medium to 30°C for biochemical Cultivate in an incubator, and measure the diameters of the bacterial rings formed by NRCB002, NRCB005, and NRCB010 at th...

Embodiment 3

[0042] Example 3 The effect of FB on the colonization of rhizosphere growth-promoting bacteria in the plant rhizosphere.

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Abstract

The invention discloses application of a high-energy phosphate compound fructose-1, 6-dicalcium phosphate (FB) to improvement of rhizosphere colonization of growth-promoting bacteria and preparation of a preparation of the growth-promoting bacteria in plant rhizosphere colonization. The signal molecule FB capable of promoting the growth-promoting bacteria to colonize at the rhizosphere is obtained through screening, and it is found that the chemotactic effect, the group migration effect and the biofilm formation of the plant rhizosphere growth-promoting bacteria are remarkably improved through the FB, and the colonization of the growth-promoting bacteria at the rhizosphere of the plant is remarkably improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to the application of fructose-1,6-diphosphate calcium salt in improving the colonization of rhizosphere growth-promoting bacteria in plant rhizosphere. Background technique [0002] Due to the excessive application of chemical fertilizers in agricultural production, a series of problems such as ecological safety, food safety, and environmental pollution have been caused. Microbial fertilizers have attracted widespread attention due to their environmental protection, safety and effectiveness, and have become a new driving force for the development of green ecological agriculture. In the past 20 years, my country's microbial fertilizer industry has experienced a stage of rapid development. At present, we have entered a period in which innovation is most needed. Research and development of new products, new strains, new processes, and new functions have become the develop...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/125C12R1/07C12R1/01C12R1/38
CPCC12N1/20Y02A40/10
Inventor 高南李珊珊应汉杰徐虹陈晓春周超伟雷鹏王瑞谷益安
Owner NANJING UNIV OF TECH