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Gene participating in synthesis of cationic peptide compound and application thereof

A technology for cationic peptides and compounds, applied in the field of genes involved in the synthesis of cationic peptide compounds

Inactive Publication Date: 2022-05-31
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies have found vancomycin and polymyxin resistance genes, which have attracted great attention worldwide, so there is an urgent need to find new antibacterial agents

Method used

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  • Gene participating in synthesis of cationic peptide compound and application thereof
  • Gene participating in synthesis of cationic peptide compound and application thereof
  • Gene participating in synthesis of cationic peptide compound and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Example 1 Acquisition of genes involved in the synthesis of cationic peptide compounds

[0099] A group of genes involved in the synthesis of cationic peptide compounds can be defined as containing 11 genes. These 11 genes are thioesterase protein gene, polyketide synthase gene, ketoreductase gene, non-ribosomal peptide synthetase gene (NRPS gene) (6 kinds), L-threonine-3-dehydrogenase gene , 2-amino-3-ketobutyrate CoA ligase gene.

[0100] Among the above-mentioned group of genes, the enzyme encoded by the NRPS gene has the function of forming the basic skeleton of the cationic peptide compound. That is, the enzyme forms a peptide backbone consisting of 13 amino acids, a fatty acid, and an aminoalcohol: [3-methyl-2-oxopentanoic acid (α-KIL)-dehydrothreonine (Thr)-methionine Acid (Met) - Ornithine (Orn) - Isoleucine (Ile) - Valine (Val) - Valine (Val) - Lysine (Lys) - Valine (Val) - Leucine acid (Leu)-lysine (Lys)-tyrosine (Tyr)-leucine (Leu)-valine (Val)]. The enzy...

Embodiment 2

[0136] Example 2 Functional evaluation of domains in each module

[0137] When the first A domain differs from the amino acid sequence of SEQ ID NO: 1, whether it can function as the A domain corresponding to 3-methyl-2-oxopentanoic acid can be evaluated as follows.

[0138] First, a mutant gene was designed to encode a first mutant A domain designed to be different from the amino acid sequence of SEQ ID NO:1. This mutant gene is expressed in an appropriate host, and it is confirmed whether a compound having the basic peptide skeleton of the above cationic peptide compound is synthesized in the host or in the metabolites in the culture supernatant. If among the metabolites there is a compound with the basic peptide backbone for the synthesis of the above-mentioned cationic peptide compounds, the designed first mutant A domain can be evaluated to function as the A domain corresponding to 3-methyl-2-oxopentanoic acid. function. Likewise, when the second A domain to the fourtee...

Embodiment 3

[0155] Example 3 Analysis of Gene Domain Structure

[0156] This example uses the antiSMASH program to analyze the domain structure of the protein (SEQ ID NO: 48-60) encoded by the gene involved in the synthesis of cationic peptide compounds of the present invention. See figure 2 ,From figure 2 It can be seen from the figure that the database in the antiSMASH program contains the structural domains in the protein encoded by the gene involved in the synthesis of cationic peptide compounds of the present invention.

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Abstract

The present invention relates to a microorganism and molecular biological technique, and more specifically, to a gene involved in the synthesis of a cationic peptide compound and a use thereof, capable of constructing a system for synthesizing a cationic peptide compound produced by Bacillus (Bacillus Cohn) bacteria, thereby making it possible to efficiently produce the cationic peptide compound.

Description

technical field [0001] The invention relates to microorganisms and molecular biology techniques, in particular to a gene involved in the synthesis of cationic peptide compounds and its application. Background technique [0002] Drug-resistant bacteria have become an important problem threatening global public health. The World Health Organization (WHO) initiative established the Global Antibiotic Research and Development Partnership (GARDP) to accelerate the development of new and improved antibiotics to combat drug-resistant bacterial infections. [0003] The commonly used antibiotics on the market are quinolones, β-lactams, macrolides and aminoglycosides, etc., but drug-resistant bacteria have become resistant to the above drugs. Later, glycopeptide and lipopeptide compounds were developed, such as vancomycin, polymyxin, daptomycin, etc., which have attracted widespread attention due to their excellent antibacterial properties. However, some studies have discovered the r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/52C12N9/00C12N15/63C12N1/21C12N1/19C07K7/08C12R1/01C12R1/19C12R1/84
CPCC12N9/93C12N15/63C07K7/08C12Y603/02Y02A50/30
Inventor 贾英民韩盼盼马爱进陈洲
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY