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Application of 1-O-gallic acid acyl-beta-D-glucoside extracted from emblic leafflower fruit in plant disease control

A technology for plant disease control, gallic acid acyl group, applied in the application, sugar derivatives, sugar derivatives, etc., can solve problems such as side effects, environmental and human health threats, reduction of potato tubers, etc.

Pending Publication Date: 2022-06-03
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although the use of chemical agents to control the disease is effective, it also has some side effects, such as reducing potato tubers, reducing yields, and posing threats to the environment and human health

Method used

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  • Application of 1-O-gallic acid acyl-beta-D-glucoside extracted from emblic leafflower fruit in plant disease control
  • Application of 1-O-gallic acid acyl-beta-D-glucoside extracted from emblic leafflower fruit in plant disease control
  • Application of 1-O-gallic acid acyl-beta-D-glucoside extracted from emblic leafflower fruit in plant disease control

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for preparing 1-O-galloacyl-β-D-glucoside from Emblica, comprising the following steps:

[0032] Step 1. Take 740g of fresh fruit of Amaryllis emblica, remove the core and squeeze the juice to obtain 234g of pomace. The pomace of Amla pomace was placed in a 5000mL beaker, soaked in 2000mL 70% ethanol and leached with ultrasonic acceleration for 1h, repeated the leaching 3 times, combined the extracts, and drained the solvent with a rotary evaporator to obtain a crude extract of the ethanol layer ( EA) 19.74 g.

[0033]Step 2. Take 19.74 g of the crude extract (EA) of the ethanol layer obtained in step 1, dissolve it in 50 mL of 50% methanol water, and use a medium and low pressure reverse phase C18 column (filler: YMC-GEL ODS-A-HG, s-50 μm ; column length is 4.5 × 35 cm), the flow rate is set to 30 mL / min, eluted with 25% methanol aqueous solution, and the 15-31 min fraction (EA-1) is collected.

[0034] Step 3. Dissolve 1.03 g of (EA-1) obtained in step 2 wi...

Embodiment 2

[0050] In the ultra-clean workbench, take the strain (Streptomyces scabies AMCC400023) preserved in the slanted medium, carefully pick a single colony from the inoculated ring after burning and cooling next to the flame of the alcohol lamp, and use the method of streaking on the plate to remove the pathogenic bacteria. Bacteria were inoculated in Gao's No. 1 solid medium, sealed with parafilm, and cultured for 48 h in a constant temperature biochemical incubator at 30 °C. Among them, Gao's No. 1 solid medium (g / L): potassium nitrate: 1.0; potassium dihydrogen phosphate: 0.5; magnesium sulfate: 0.5; ferrous sulfate: 0.01; sodium chloride: 0.5; soluble starch: 20.0; agar : 15.0.

[0051] Pick the activated single colony of Streptomyces scabtus with an inoculation loop, put it on Gao's No. 1 medium, and keep it at a constant temperature of 28 °C for 3 days; suck 3 mL of sterile water on the plate, gently with a blade Scrape off the surface spores, rinse the medium surface spores...

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PUM

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Abstract

The invention discloses an application of 1-O-gallic acid acyl-beta-D-glucoside extracted from emblic leafflower fruit in prevention and treatment of plant diseases, wherein the plant diseases are potato scab. The 1-O-gallic acid acyl-beta-D-glucoside is obtained by extracting, separating and purifying phyllanthus emblica pomace, the 1-O-gallic acid acyl-beta-D-glucoside is found to have the effect of inhibiting the activity of streptomyces scabies for the first time, and the 1-O-gallic acid acyl-beta-D-glucoside can be used for controlling potato scab.

Description

technical field [0001] The invention relates to the technical field of active medicines, in particular to the application of 1-O-galloacyl-β-D-glucoside extracted from Emblica radix in plant disease prevention and control. Background technique [0002] Emblica (phyllanthus emblica L.) is the fruit of the genus Phyllanthus in the family Euphorbiaceae. Mainly distributed in the tropics and subtropics; native to India, distributed in Pakistan, Malaysia, China and other countries; Fujian and Yunnan provinces have the largest production. As early as 1998, it was included in the list of "both food and medicine" by the Ministry of Health, which was included in multiple editions of the "Pharmacopoeia of the People's Republic of China", and was designated by the United Nations Health Organization as 3 kinds of health care plants to be promoted and grown worldwide. one. The special composition and pharmacological activity of Amla amla has a very good development and application valu...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01P1/00C07H13/08C07H1/08
CPCA01N43/16C07H13/08C07H1/08
Inventor 李健吴秀娜张铃玉吴达仁
Owner JIMEI UNIV