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Immunomodulator for treating colorectal cancer lung metastasis and composition for predicting and detecting immunomodulator

An immunomodulator, colorectal cancer technology, applied in the direction of microbial measurement/testing, drug combination, microbial-based methods, etc., can solve the problems of hard to find, no clinical specimens, and few research reports on colorectal cancer lung metastasis, etc. Achieve the effect of short time and simple detection method

Pending Publication Date: 2022-06-07
SUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Secondly, the current clinical treatment methods for colorectal cancer liver metastasis are relatively complete, but there are few research reports on colorectal cancer lung metastasis
At present, the great defect in the study of biological rhythm is that there is no suitable clinical specimen of abnormal biological rhythm, and it is difficult to trace the source of many diseases in the later stage of development. Whether it is related to biological rhythm
From the perspective of current medical costs, it is impossible to perform whole-genome sequencing of each organism to detect whether the biological clock gene is defective

Method used

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  • Immunomodulator for treating colorectal cancer lung metastasis and composition for predicting and detecting immunomodulator
  • Immunomodulator for treating colorectal cancer lung metastasis and composition for predicting and detecting immunomodulator
  • Immunomodulator for treating colorectal cancer lung metastasis and composition for predicting and detecting immunomodulator

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Bmal1- / - mice and control wild-type mice were simultaneously induced to model colorectal cancer lung metastasis. Among them, the specific method of inducing the colorectal cancer lung metastasis model is as follows: select M38 mouse colorectal cancer cells, and the injected cell volume is 1 × 10 6 per mouse. The observation time was 18 days, and the animals were dissected on the 18th day after injection by means of tail vein injection. Bmal1 - / - Mice were purchased from Jackson Laborary, USA, B6.129-Arntltm1Bra / J, Strain#:009100), Per1 - / - Mice were purchased from Jackson Laborary, USA, B6.BTBR-Tg(Per1-luc,Per1)1Jt / J, Strain#:016187; Per2 - / - Mice were purchased from Jackson Laborary, USA, Per2- / - were purchased from Jackson Laborary, USA, B6(129S6)-Per2em2Vrshp / J, Strain#: 035521.

[0038] It turns out that Bmal1 - / - mouse, Per1 - / - Per2 - / - Mice are more prone to colorectal cancer lung metastases, see figure 1 . Panel A is the macroscopic observation of the W...

Embodiment 2

[0040] Lymphocytes were isolated from mouse lung metastases and labelled MDSC machine subsets for flow cytometry analysis. Do a point histogram of the analyzed data, and perform p-value analysis. see the results figure 2 ,in. Panel A is a WT mouse, Bmal1 - / - Mice and Per1 - / - Per2 - / - Flow cytometry results of MDSC cells in mouse lung metastases; panel B is a WT mouse, Bmal1 - / - Mice and Per1 - / - Per2 - / - MDSC cells, M-MDSC subsets (Ly6C high Ly6G - ) and PMN-MDSC subsets (Ly6C low Ly6G + ); panel C is the expression of PDL1 in M-MDSC and PMN-MDSC.

[0041] It was found that Per1 - / - Per2 - / - , Bmal1 - / - MDSC (CD11b) in mouse lung metastases + Gr-1 + ) cells numbered significantly more than wild type, where Per1 - / - Per2 - / - M-MDSC (Ly6C high Ly6G - ), Bmal1 - / - Compared with wild type, PMN-MDSC (Ly6C low Ly6G + ), Kejian, Biorhythm disorders affect the increase in the proportion of MDSCs in the lung, among which, the rhythm disorder caused by the delet...

Embodiment 3

[0043] Three groups of mouse feces were collected, each group was 5-7 mouse feces, 1 g of each mouse feces was collected, sent on dry ice, and 16SDNA flora sequencing was performed. see the results image 3 .

[0044] image 3 showed that Bacteroides lachnoclostridium was found to be in Per1 - / - Per2 - / - The abundance of intestinal bacteria in mice was significantly higher than that in the wild-type control group; Helicobacter Helicobacter in Bmal1 - / - and Per1 - / - Per2 - / - The abundance of intestinal bacteria in the mice was significantly higher than that in the control group.

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Abstract

The invention relates to an immunomodulator for treating lung metastasis of colorectal cancer and a composition for prediction and detection of the immunomodulator, the immunomodulator comprises a substance for inhibiting deletion of a Bmal1 gene and / or a substance for inhibiting deletion of a Per1 gene and a Per2 gene, and the composition for predicting or detecting lung metastasis of colorectal cancer comprises a reagent for detecting Helicobacter helicobacter and Lacnoctustridium bacteroides, a reagent for detecting Helicobacter helicobacter and Lacnoctustridium bacteroides, and a reagent for predicting or detecting lung metastasis of colorectal cancer. And / or a reagent for detecting bile acid taurocholic acid. The development process of colorectal cancer lung metastasis is influenced by adjusting the tumor immune microenvironment, the risk of colorectal cancer lung metastasis is predicted by detecting the abundance of intestinal bacteria and metabolites thereof, the detection mode is simple and convenient, and accurate detection is convenient to achieve.

Description

technical field [0001] The present invention relates to the technical field of disease treatment and detection, in particular to an immunomodulator for the treatment of colorectal cancer lung metastasis and a composition for prediction and detection thereof. Background technique [0002] Colorectal cancer is one of the top three malignant tumors in the world. Although the diagnosis and treatment technology has been greatly improved, the prognosis of colorectal cancer patients with distant metastasis is still poor. In recent years, the successful application of comprehensive therapy in the field of liver metastases has significantly improved the survival of patients with liver metastases. Compared with colorectal cancer liver metastasis, the level of attention, comprehensive treatment concept, and related clinical and basic research on colorectal cancer lung metastasis are relatively lacking. In addition, the proportion of rectal cancer in my country is nearly 50%, and recta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K45/06A61P35/04A61P37/02C12Q1/6869C12Q1/689C12R1/01
CPCA61K45/00A61K45/06A61P35/04A61P37/02C12Q1/6869C12Q1/689C12Q2535/122Y02A50/30
Inventor 李建明沈彤刘婧琳优鲁土孜阿依·日夏提王楚怡张子龙陈文舒徐旭
Owner SUZHOU UNIV
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