Specific antibody of new coronavirus S protein RBD region as well as preparation method and application of specific antibody

Abstract

The invention relates to an antigen binding protein, an antibody or an antibody active fragment obtained by immunizing a camelidae animal by using an S protein RBD region of SARS-Cov-2. According to the invention, the antibody which specifically recognizes and is combined with the S protein RBD region on the surface of the new coronavirus is screened, identified and prepared by relying on the immune system of a camelidae animal, and the obtained antibody has the advantages of strong specificity, high affinity, small relative molecular mass, high solubility, strong tissue permeability, good distribution, rapid diffusion and metabolism, simple structure, easiness in genetic engineering modification and wide application prospect. And the recombinant plasmid is simple in humanization, high in stability, resistant to acid and alkali pH, resistant to high temperature, relatively low in mass production cost and easy to recombine and prepare on a large scale, and has potential clinical diagnosis and treatment values.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a specific antibody for the RBD region of the S protein of the novel coronavirus, and a preparation method and application thereof. Background technique [0002] Antibodies are proteins mainly secreted by plasma cells and used by the immune system to identify and neutralize foreign substances such as bacteria and viruses, which are called antigens. The binding of antibodies and antigens is entirely dependent on non-covalent interactions. This specific binding mechanism allows antibodies to capture foreign microorganisms as well as infected cells, further inducing other immune mechanisms to attack them, or directly neutralize their targets . Antibodies and antibody-related products have been widely used in research fields such as life science and medicine. Many experimental techniques derived from antigen-antibody specific binding have laid an important foundation for scientific rese...

AI Technical Summary

Problems solved by technology

[0004] The above-mentioned technology based on the immune system of animals such as mice to generate and separate antibodies to the S protein on the surface of the new coronavirus has the following disadvantages: (1) The specificity of the antibody is limited: the S protein has a large structure and complex spatial folding. Traditional mouse monoclonal antibodies It is difficult to identify the complex spatial structure of the S surface, and it is difficult for researchers to obtain antibodies against each domain; (2) The stability of the antibody is poor: the above-mentioned mouse monoclonal antibody needs to be stored, transported, tested and contacted at low temperature (4 degrees Celsius). Verification and other work can maintain the concentration, specificity and other characteristics of the antibody, which is not conducive to the development of large-scale application research; (3) large-scale mass production of finished products: the above-mentioned mouse monoclonal antibody is a full-length immunoglobulin, which needs to be produced in mammals Cells and other expensive expression systems carry out recombinant expression followed by separation and purification. The operation is complicated and expensive, which is not conducive to large-scale mass production

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