Method for detecting methyl mercury based on gold nano-enzyme peroxidase
A technology of nano-enzyme peroxidase and methylmercury, which is applied in the direction of nano-carbon, nano-technology, nano-optics, etc., can solve the problems of unstable method, poor reproducibility, and easy aggregation, so as to avoid uneven color and carry Convenience, Analytical Sensitive Effects
Pending Publication Date: 2022-06-07
KUNMING UNIV OF SCI & TECH +1
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Abstract
According to the method, serotonin carbon dots and glucose are used as a reducing agent and a stabilizing agent to prepare carbon dot gold nano-enzyme, the carbon dot gold nano-enzyme has the characteristics and reducibility of quasi-peroxidase nano-enzyme and can catalyze reduction of methyl mercury, and due to the fact that the absolute electronegativity of Hg is smaller than that of Au, the carbon dot gold nano-enzyme can catalyze reduction of methyl mercury. Electrons transferred from Au-Hg amalgam are much easier than electrons transferred from Au, so that the catalytic activity of the nano-enzyme is enhanced after methyl mercury is introduced, TMB can be quickly oxidized into a blue oxidation product oxTMB by H2O2, on the basis of the principle, methyl mercury is quickly detected, a test strip capable of being used for on-site quick detection can be prepared, detection of the test strip is completed within 10 minutes, and the detection efficiency is greatly improved. The limit of quantitation of methyl mercury reaches 0.5 g/kg. The method has the characteristics of high specificity, convenience in carrying, rapidness, low cost, sensitivity in analysis and the like.
Application Domain
Material analysis by observing effect on chemical indicatorNanooptics +2
Technology Topic
MethylmercurySerotonin +9
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