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Method for detecting methyl mercury based on gold nano-enzyme peroxidase

A technology of nano-enzyme peroxidase and methylmercury, which is applied in the direction of nano-carbon, nano-technology, nano-optics, etc., can solve the problems of unstable method, poor reproducibility, and easy aggregation, so as to avoid uneven color and carry Convenience, Analytical Sensitive Effects

Pending Publication Date: 2022-06-07
KUNMING UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Unlike inorganic mercury, the oxidation / reduction of methylmercury has not been fully studied so far in the development of portable nanosensors. Colorimetric determination method, but the method is unstable and poor reproducibility. At the same time, although the gold nano sol is easy to prepare and easy to operate in the sensing process, it has the problem of easy aggregation during long-term storage

Method used

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  • Method for detecting methyl mercury based on gold nano-enzyme peroxidase
  • Method for detecting methyl mercury based on gold nano-enzyme peroxidase
  • Method for detecting methyl mercury based on gold nano-enzyme peroxidase

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Example 1: Rapid detection of methylmercury in fish by carbon dot gold nanozyme

[0031] (1) Weigh 2.0g of serotonin and 1.0g of glucose and dissolve them in 20mL of ultrapure water, ultrasonically mix evenly, transfer the solution to a polytetrafluoroethylene-lined hydrothermal reactor, and heat it at a constant temperature of 200 °C for 6 hours to react. After completion, it was naturally cooled to room temperature to obtain a brown solution; the brown solution was removed with a 0.22 μm filter membrane to remove large particles of impurities, and then centrifuged at 8000 r / min, and the supernatant was dried in vacuum at 60 °C to obtain serotonin carbon dots;

[0032] (2) Dissolve 12 mg of serotonin carbon dots in 40 mL of ultrapure water, heat the oil bath to 100°C, and add HAuCl 4 , HAuCl 4 The concentration in the mixed solution was 10 mg / mL, stirred for 60 minutes in the dark, cooled to room temperature, centrifuged at 8000 r / min, and the supernatant was dried to...

Embodiment 2

[0048] Example 2: Rapid detection of methylmercury in environmental water samples by carbon dot gold nanozyme

[0049] (1) Weigh 3.0g of serotonin and 2.0g of glucose and dissolve them in 30mL of ultrapure water, ultrasonically mix evenly, transfer the solution to a polytetrafluoroethylene-lined hydrothermal reactor, and heat it at a constant temperature of 200 °C for 6 hours to react. After completion, it was naturally cooled to room temperature to obtain a brown solution; the brown solution was used to remove large particles of impurities with a 0.22 μm filter membrane, and then centrifuged at 8000 r / min, and the supernatant was vacuum-dried at 60 °C to obtain serotonin carbon dots;

[0050] (2) Dissolve 15 mg of serotonin carbon dots in 50 mL of ultrapure water, heat the oil bath to 100 °C, and add HAuCl 4 , HAuCl 4 The concentration in the mixed solution was 12 mg / mL, and the mixture was stirred for 60 minutes in the dark, cooled to room temperature, centrifuged at 8000 r...

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Abstract

According to the method, serotonin carbon dots and glucose are used as a reducing agent and a stabilizing agent to prepare carbon dot gold nano-enzyme, the carbon dot gold nano-enzyme has the characteristics and reducibility of quasi-peroxidase nano-enzyme and can catalyze reduction of methyl mercury, and due to the fact that the absolute electronegativity of Hg is smaller than that of Au, the carbon dot gold nano-enzyme can catalyze reduction of methyl mercury. Electrons transferred from Au-Hg amalgam are much easier than electrons transferred from Au, so that the catalytic activity of the nano-enzyme is enhanced after methyl mercury is introduced, TMB can be quickly oxidized into a blue oxidation product oxTMB by H2O2, on the basis of the principle, methyl mercury is quickly detected, a test strip capable of being used for on-site quick detection can be prepared, detection of the test strip is completed within 10 minutes, and the detection efficiency is greatly improved. The limit of quantitation of methyl mercury reaches 0.5 g / kg. The method has the characteristics of high specificity, convenience in carrying, rapidness, low cost, sensitivity in analysis and the like.

Description

technical field [0001] The invention relates to the technical field of chemical analysis and detection, in particular to a method and a test strip for detecting methylmercury based on gold nano-enzyme peroxidase. Background technique [0002] Mercury is one of the major pollutants that exists widely in the world. As a kind of organic mercury, methylmercury is the most toxic and fat-soluble. It is easily absorbed and enriched by organisms in water bodies. Mercury concentrations can reach 10 4 ~10 6 times. Long-term consumption of food contaminated with mercury will cause chronic poisoning. Minamata disease in Japan is caused by long-term consumption of contaminated fish and shellfish. Therefore, the determination of methylmercury in aquatic products is very necessary. At present, the relevant national methylmercury detection methods include gas chromatography-inductively coupled plasma mass spectrometry (GC-ICP-MS) method, high performance liquid chromatography-inductivel...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/78B22F9/24C01B32/15B82Y20/00B82Y40/00
CPCG01N21/31G01N21/78B22F9/24C01B32/15B82Y20/00B82Y40/00G01N2021/7759G01N2021/775B22F2301/255
Inventor 杨德志李秋兰魏大巧尹孟佳杨亚玲
Owner KUNMING UNIV OF SCI & TECH
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